3D investigation shows walls and wall-like structures around human germinal centres, probably regulating T- and B-cell entry and exit
- This study deals with 3D laser investigation on the border between the human lymph node T-zone and germinal centre. Only a few T-cells specific for antigen selected B-cells are allowed to enter germinal centres. This selection process is guided by sinus structures, chemokine gradients and inherent motility of the lymphoid cells. We measured gaps and wall-like structures manually, using IMARIS, a 3D image software for analysis and interpretation of microscopy datasets. In this paper, we describe alpha-actin positive and semipermeable walls and wall-like structures that may hinder T-cells and other cell types from entering germinal centres. Some clearly defined holes or gaps probably regulate lymphoid traffic between T- and B-cell areas. In lymphadenitis, the morphology of this border structure is clearly defined. However, in case of malignant lymphoma, the wall-like structure is disrupted. This has been demonstrated exemplarily in case of angioimmunoblastic T-cell lymphoma. We revealed significant differences of lengths of the wall-like structures in angioimmunoblastic T-cell lymphoma in comparison with wall-like structures in reactive tissue slices. The alterations of morphological structures lead to abnormal and less controlled T- and B-cell distributions probably preventing the immune defence against tumour cells and infectious agents by dysregulating immune homeostasis.
Verfasserangaben: | Miguel Thomos, Patrick WurzelORCiDGND, Sonja ScharfORCiDGND, Ina KochORCiD, Martin-Leo HansmannGND |
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URN: | urn:nbn:de:hebis:30:3-623959 |
DOI: | https://doi.org/10.1371/journal.pone.0242177 |
ISSN: | 1932-6203 |
Titel des übergeordneten Werkes (Englisch): | PLoS ONE |
Verlag: | PLOS |
Verlagsort: | San Francisco, California, US |
Dokumentart: | Wissenschaftlicher Artikel |
Sprache: | Englisch |
Datum der Veröffentlichung (online): | 10.11.2020 |
Datum der Erstveröffentlichung: | 10.11.2020 |
Veröffentlichende Institution: | Universitätsbibliothek Johann Christian Senckenberg |
Datum der Freischaltung: | 17.08.2021 |
Jahrgang: | 15 |
Ausgabe / Heft: | 11, art. e0242177 |
Seitenzahl: | 11 |
Erste Seite: | 1 |
Letzte Seite: | 11 |
HeBIS-PPN: | 487214765 |
Institute: | Medizin / Medizin |
DDC-Klassifikation: | 6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit |
Sammlungen: | Universitätspublikationen |
Open-Access-Publikationsfonds: | Medizin |
Lizenz (Deutsch): | ![]() |