Nueva técnica de microscopía electrónica de barrido para el estudio de Coccoidea (Insecta: Hemiptera)

  • A new protocol to prepare material of Coccoidea (Insecta: Hemiptera) for observation with scanning electron microscopy is presented. This technique consists of cleaning samples (stored in 96% alcohol) with acetone and xylene, taking them directly to the hydration step, and subsequently dehydrating them in alcohol. Fixation in glutaraldehyde and post-fi xation in osmium tetroxide are omitted. The cleaning of the samples and the correct critical point dryer are very important.

Download full text files

Export metadata

Additional Services

Share in Twitter Search Google Scholar
Author:Andrea Saracho Bottero, Lucía E. Claps
Parent Title (English):Insecta mundi : a journal of world insect systematics
Series (Serial Number):Insecta Mundi (0446)
Publisher:Center for Systematic Entomology
Place of publication:Gainesville, Fla.
Document Type:Part of Periodical
Date of Publication (online):2016/05/19
Year of first Publication:2015
Publishing Institution:Universitätsbibliothek Johann Christian Senckenberg
Release Date:2016/05/19
Tag:Dactylopiidae; Pseudococcidae; SEM
Page Number:8
First Page:1
Last Page:5
Dewey Decimal Classification:5 Naturwissenschaften und Mathematik / 59 Tiere (Zoologie) / 590 Tiere (Zoologie)
Sammlungen:Sammlung Biologie / Sondersammelgebiets-Volltexte
Licence (German):License LogoCreative Commons - Namensnennung-Nicht kommerziell 3.0