Heterogeneous regulation of bacterial natural product biosynthesis via a novel transcription factor
- Biological diversity arises among genetically equal subpopulations in the same environment, a phenomenon called phenotypic heterogeneity. The life cycle of the enteric bacterium Photorhabdus luminescens involves a symbiotic interaction with nematodes as well as a pathogenic association with insect larvae. P. luminescens exists in two distinct phenotypic forms designated as primary (1°) and secondary (2°). In contrast to 1° cells, 2° cells are non-pigmented due to the absence of natural compounds, especially anthraquinones (AQs). We identified a novel type of transcriptional regulator, AntJ, which activates expression of the antA-I operon responsible for AQ production. AntJ heterogeneously activates the AQ production in single P. luminescens 1° cells, and blocks AQ production in 2° cells. AntJ contains a proposed ligand-binding WYL-domain, which is widespread among bacteria. AntJ is one of the rare examples of regulators that mediate heterogeneous gene expression by altering activity rather than copy number in single cells.
Author: | Antje Katharina Heinrich, Angela Glaeser, Nicholas J. TobiasORCiD, Ralf Heermann, Helge Björn BodeORCiDGND |
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URN: | urn:nbn:de:hebis:30:3-436782 |
DOI: | https://doi.org/10.1016/j.heliyon.2016.e00197 |
ISSN: | 2405-8440 |
Pubmed Id: | https://pubmed.ncbi.nlm.nih.gov/27957552 |
Parent Title (English): | Heliyon |
Publisher: | Elsevier |
Place of publication: | London [u. a.] |
Document Type: | Article |
Language: | English |
Date of Publication (online): | 2017/06/26 |
Year of first Publication: | 2016 |
Publishing Institution: | Universitätsbibliothek Johann Christian Senckenberg |
Release Date: | 2017/06/26 |
Tag: | Cell biology; Genetics; Microbiology |
Volume: | 2 |
Issue: | 11, e00197 |
Page Number: | 32 |
First Page: | 1 |
Last Page: | 32 |
Note: | © 2016 The Authors. Published by Elsevier Ltd. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
HeBIS-PPN: | 428726437 |
Institutes: | Biowissenschaften / Biowissenschaften |
Dewey Decimal Classification: | 5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie |
Sammlungen: | Universitätspublikationen |
Licence (German): | Creative Commons - Namensnennung-Nicht kommerziell - Keine Bearbeitung 4.0 |