In the complex family of heat stress transcription factors, HsfA1 has a unique role as master regulator of thermotolerance in tomato
- We generated transgenic tomato plants with altered expression of heat stress transcription factor HsfA1. Plants with 10-fold overexpression of HsfA1 (OE plants) were characterized by a singleHsfA1 transgene cassette, whereas plants harboring a tandem inverted repeat of the cassette showed cosuppression (CS plants) by posttranscriptional silencing of the HsfA1 gene connected with formation of small interfering RNAs. Under normal growth conditions, major developmental parameters were similar for wild-type (WT), OE, and CS plants. However, CS plants and fruits were extremely sensitive to elevated temperatures, because heat stress-induced synthesis of chaperones and Hsfs was strongly reduced or lacking. Despite the complexity of the plant Hsf family with at least 17 members in tomato, HsfA1 has a unique function as master regulator for induced thermotolerance. Using transient reporter assays with mesophyll protoplasts from WT tomato, we demonstrated that plasmid-encoded HsfA1 and HsfA2 were well expressed. However, in CS protoplasts the cosuppression phenomenon was faithfully reproduced. Only transformation with HsfA2 expression plasmid led to normal expression of the transcription factor and reporter gene activation, whereas even high amounts of HsfA1 expression plasmids were silenced. Thermotolerance in CS protoplasts was restored by plasmid-borne HsfA2, resulting in expression of chaperones, thermoprotection of firefly luciferase, and assembly of heat stress granules.
Author: | Shravan Kumar MishraORCiDGND, Joanna TrippORCiDGND, Sybille WinkelhausGND, Bettina Tschiersch, Klaus TheresORCiDGND, Lutz NoverGND, Klaus-Dieter Scharf |
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URN: | urn:nbn:de:hebis:30:3-456155 |
DOI: | https://doi.org/10.1101/gad.228802 |
ISSN: | 1549-5477 |
ISSN: | 0890-9369 |
Pubmed Id: | https://pubmed.ncbi.nlm.nih.gov/12080093 |
Parent Title (English): | Genes & development |
Publisher: | HighWire PressCold ; Laboratory Press |
Place of publication: | Stanford, Calif. ; Spring Harbor, NY |
Document Type: | Article |
Language: | English |
Year of Completion: | 2002 |
Date of first Publication: | 2002/06/15 |
Publishing Institution: | Universitätsbibliothek Johann Christian Senckenberg |
Release Date: | 2018/02/06 |
Tag: | Posttranscriptional gene silencing; chaperones; heat stress transcription factors; siRNA; thermotolerance; tomato |
Volume: | 16 |
Issue: | 12 |
Page Number: | 14 |
First Page: | 1555 |
Last Page: | 1567 |
Note: | Articles not designated as Open Access are distributed exclusively by Cold Spring Harbor Laboratory Press for the first six months after the full-issue publication date (see Terms for complete details). After six months, they are available under a Creative Commons License (Attribution-NonCommercial 4.0 International License). |
HeBIS-PPN: | 427780586 |
Institutes: | Biowissenschaften / Biowissenschaften |
Dewey Decimal Classification: | 5 Naturwissenschaften und Mathematik / 55 Geowissenschaften, Geologie / 550 Geowissenschaften |
Sammlungen: | Universitätspublikationen |
Licence (English): | Creative Commons - Namensnennung-Nicht kommerziell 4.0 |