Cytotoxicity of self-etch versus etch-and-rinse dentin adhesives: A screening study

  • Six dentin adhesives were tested in vitro regarding their cytotoxicity on human fibroblasts. The adhesives Hybrid Bond, One-up Bond F Plus, AdheSE, Clearfil SE Bond, Optibond Solo Plus and Syntac were eluted with culture medium as single or sequentially applied adhesive part for 24 h. 75 Petri dishes were produced per group. They were evaluated triangulated, comprising the quantitative evaluation (105 ones) to determine “viable”, “dead” and “debris” cells with the use of a cell-counter and the reactivity index was also identified based on the qualitative assessment (420 ones). One-up Bond F Plus, AdheSE and Clearfil SE Bond showed a statistical difference of viable cells to the cell control. For One-up Bond F Plus, statistically, differences compared to hybrid bond and Syntac were also found. All the adhesives except One-up Bond F Plus showed significant differences between single and sequentially applied adhesive part regarding the quantitative evaluation. The test material showed a moderate grade of cytotoxicity. As a result, a statistically significant difference of the cytotoxicity between the self-etch and etch-and-rinse adhesives cannot be demonstrated regarding the qualitative evaluation and the reactivity index, but the differences between sequentially applied and single applied components can be proved.

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Author:Luisa Fröb, Stefan RüttermannORCiDGND, Georgios RomanosORCiDGND, Eva HerrmannORCiDGND, Susanne Gerhardt-SzépGND
Pubmed Id:
Parent Title (English):Materials
Place of publication:Basel
Document Type:Article
Year of Completion:2020
Date of first Publication:2020/01/17
Publishing Institution:Universitätsbibliothek Johann Christian Senckenberg
Release Date:2020/03/11
Tag:cells; cytotoxicity; dentin adhesives; fibroblasts; in vitro; screening
Issue:2, Art. 452
Page Number:17
First Page:1
Last Page:17
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited
Institutes:Medizin / Medizin
Dewey Decimal Classification:6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit
Licence (German):License LogoCreative Commons - Namensnennung 4.0