Real‐time NMR spectroscopy for studying metabolism

  • Current metabolomics approaches utilize cellular metabolite extracts, are destructive, and require high cell numbers. We introduce here an approach that enables the monitoring of cellular metabolism at lower cell numbers by observing the consumption/production of different metabolites over several kinetic data points of up to 48 hours. Our approach does not influence cellular viability, as we optimized the cellular matrix in comparison to other materials used in a variety of in‐cell NMR spectroscopy experiments. We are able to monitor real‐time metabolism of primary patient cells, which are extremely sensitive to external stress. Measurements are set up in an interleaved manner with short acquisition times (approximately 7 minutes per sample), which allows the monitoring of up to 15 patient samples simultaneously. Further, we implemented our approach for performing tracer‐based assays. Our approach will be important not only in the metabolomics fields, but also in individualized diagnostics.
  • Klare Sicht auf die Dinge: Ein neu entwickelter Ansatz zur Überwachung des Stoffwechsels von Lebendzellen in einer zellfreundlichen Umgebung wird vorgestellt und rückt somit die NMR‐Spektroskopie näher an personalisierte Medizin.

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Author:Islam Alshamleh, Nina Krause, Christian RichterORCiDGND, Nina Susanne KurrleORCiDGND, Hubert ServeORCiDGND, Ulrich L. Günther, Harald SchwalbeORCiDGND
URN:urn:nbn:de:hebis:30:3-533458
DOI:https://doi.org/10.1002/ange.201912919
ISSN:1521-3757
ISSN:0044-8249
Parent Title (German):Angewandte Chemie
Publisher:Wiley-VCH
Place of publication:Weinheim
Document Type:Article
Language:English
Year of Completion:2019
Date of first Publication:2019/11/15
Publishing Institution:Universitätsbibliothek Johann Christian Senckenberg
Release Date:2020/04/22
Tag:Biochemie; Echtzeit-NMR-Spektroskopie; Personalisierte Medizin; Stoffwechsel; Zellstudien
Volume:132
Issue:6
Page Number:5
First Page:2324
Last Page:2328
Note:
This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
Note:
Auch als "internationale Ausgabe" erschienen: Angewandte Chemie. International edition, 59.2020, Nr. 6, S. 2304-2308, doi:10.1002/anie.201912919
HeBIS-PPN:465920780
Institutes:Biochemie, Chemie und Pharmazie / Biochemie und Chemie
Exzellenzcluster / Exzellenzcluster Makromolekulare Komplexe
Wissenschaftliche Zentren und koordinierte Programme / Sonderforschungsbereiche / Forschungskollegs
Wissenschaftliche Zentren und koordinierte Programme / Zentrum für Biomolekulare Magnetische Resonanz (BMRZ)
Dewey Decimal Classification:5 Naturwissenschaften und Mathematik / 54 Chemie / 540 Chemie und zugeordnete Wissenschaften
Sammlungen:Universitätspublikationen
Licence (German):License LogoCreative Commons - Namensnennung 4.0