3d-printing of hierarchically designed and osteoconductive bone tissue engineering scaffolds

  • In Bone Tissue Engineering (BTE), autologous bone-regenerative cells are combined with a scaffold for large bone defect treatment (LBDT). Microporous, polylactic acid (PLA) scaffolds showed good healing results in small animals. However, transfer to large animal models is not easily achieved simply by upscaling the design. Increasing diffusion distances have a negative impact on cell survival and nutrition supply, leading to cell death and ultimately implant failure. Here, a novel scaffold architecture was designed to meet all requirements for an advanced bone substitute. Biofunctional, porous subunits in a load-bearing, compression-resistant frame structure characterize this approach. An open, macro- and microporous internal architecture (100 µm–2 mm pores) optimizes conditions for oxygen and nutrient supply to the implant’s inner areas by diffusion. A prototype was 3D-printed applying Fused Filament Fabrication using PLA. After incubation with Saos-2 (Sarcoma osteogenic) cells for 14 days, cell morphology, cell distribution, cell survival (fluorescence microscopy and LDH-based cytotoxicity assay), metabolic activity (MTT test), and osteogenic gene expression were determined. The adherent cells showed colonization properties, proliferation potential, and osteogenic differentiation. The innovative design, with its porous structure, is a promising matrix for cell settlement and proliferation. The modular design allows easy upscaling and offers a solution for LBDT.

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Author:Nicolas SöhlingGND, Jonas NeijhoftORCiDGND, Vinzenz Nienhaus, Valentin Acker, Jana Harbig, Fabian Menz, Joachim Ochs, René VerboketORCiDGND, Ulrike Ritz, Andreas Blaeser, Edgar Dörsam, Johannes FrankORCiDGND, Ingo MarziORCiDGND, Dirk HenrichORCiDGND
Parent Title (English):Materials
Place of publication:Basel
Document Type:Article
Date of Publication (online):2020/04/13
Date of first Publication:2020/04/13
Publishing Institution:Universitätsbibliothek Johann Christian Senckenberg
Release Date:2020/05/07
Page Number:18
First Page:1
Last Page:18
Institutes:Medizin / Medizin
Dewey Decimal Classification:6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit
Licence (German):License LogoCreative Commons - Namensnennung 4.0