Legionella shows a diverse secondary metabolism dependent on a broad spectrum Sfp-type phosphopantetheinyl transferase

  • Several members of the genus Legionella cause Legionnaires’ disease, a potentially debilitating form of pneumonia. Studies frequently focus on the abundant number of virulence factors present in this genus. However, what is often overlooked is the role of secondary metabolites from Legionella. Following whole genome sequencing, we assembled and annotated the Legionella parisiensis DSM 19216 genome. Together with 14 other members of the Legionella, we performed comparative genomics and analysed the secondary metabolite potential of each strain. We found that Legionella contains a huge variety of biosynthetic gene clusters (BGCs) that are potentially making a significant number of novel natural products with undefined function. Surprisingly, only a single Sfp-like phosphopantetheinyl transferase is found in all Legionella strains analyzed that might be responsible for the activation of all carrier proteins in primary (fatty acid biosynthesis) and secondary metabolism (polyketide and non-ribosomal peptide synthesis). Using conserved active site motifs, we predict some novel compounds that are probably involved in cell-cell communication, differing to known communication systems. We identify several gene clusters, which may represent novel signaling mechanisms and demonstrate the natural product potential of Legionella.

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Author:Nicholas J. TobiasORCiD, Tilman Ahrendt, Ursula Schell, Melissa Miltenberger, Hubert Hilbi, Helge Björn BodeORCiDGND
Pubmed Id:https://pubmed.ncbi.nlm.nih.gov/27904811
Parent Title (German):PeerJ
Publisher:PeerJ Inc.
Place of publication:London [u.a.]
Document Type:Article
Date of Publication (online):2016/11/24
Date of first Publication:2016/11/24
Publishing Institution:Universitätsbibliothek Johann Christian Senckenberg
Release Date:2020/05/12
Tag:legionella; natural products; non-ribosomal peptide synthetase; polyketide synthase; secondary metabolism
Page Number:22
Institutes:Biowissenschaften / Biowissenschaften
Dewey Decimal Classification:5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie
Licence (German):License LogoCreative Commons - Namensnennung 4.0