Microbiome patterns in matched bile, duodenal, pancreatic tumor tissue, drainage, and stool samples: association with preoperative stenting and postoperative pancreatic fistula development

  • Postoperative complications after pancreatic surgery are still a significant problem in clinical practice. The aim of this study was to characterize and compare the microbiomes of different body compartments (bile duct, duodenal mucosa, pancreatic tumor lesion, postoperative drainage fluid, and stool samples; preoperative and postoperative) in patients undergoing pancreatic surgery for suspected pancreatic cancer, and their association with relevant clinical factors (stent placement, pancreatic fistula, and gland texture). For this, solid (duodenal mucosa, pancreatic tumor tissue, stool) and liquid (bile, drainage fluid) biopsy samples of 10 patients were analyzed using 16s rRNA gene next-generation sequencing. Our analysis revealed: (i) a distinct microbiome in the different compartments, (ii) markedly higher abundance of Enterococcus in patients undergoing preoperative stent placement in the common bile duct, (iii) significant differences in the beta diversity between patients who developed a postoperative pancreatic fistula (POPF B/C), (iv) patients with POPF B/C were more likely to have bacteria belonging to the genus Enterococcus, and (v) differences in microbiome composition with regard to the pancreatic gland texture. The structure of the microbiome is distinctive in different compartments, and can be associated with the development of a postoperative pancreatic fistula.

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Author:Melanie Langheinrich, Stefan Wirtz, Barbara Kneis, Matthias M. Gittler, Olaf Tyc, Robert SchierwagenORCiDGND, Maximilian Brunner, Christian Krautz, Georg F. Weber, Christian Pilarsky, Jonel TrebickaORCiDGND, Abbas Agaimy, Robert Grützmann, Stephan Kersting
Parent Title (English):Journal of Clinical Medicine
Place of publication:Basel
Document Type:Article
Date of Publication (online):2020/08/28
Date of first Publication:2020/08/28
Publishing Institution:Universitätsbibliothek Johann Christian Senckenberg
Release Date:2020/11/05
Tag:16S rRNA sequencing; microbiome; pancreatic cancer; pancreatic surgery; postoperative complications
Issue:(9), 2785
Page Number:17
Institutes:Medizin / Medizin
Dewey Decimal Classification:6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit
Licence (German):License LogoCreative Commons - Namensnennung 4.0