Identification and regulation of tomato Serine/Arginine-rich proteins under high temperatures

  • Alternative splicing is an important mechanism for the regulation of gene expression in eukaryotes during development, cell differentiation or stress response. Alterations in the splicing profiles of genes under high temperatures that cause heat stress (HS) can impact the maintenance of cellular homeostasis and thermotolerance. Consequently, information on factors involved in HS-sensitive alternative splicing is required to formulate the principles of HS response. Serine/arginine-rich (SR) proteins have a central role in alternative splicing. We aimed for the identification and characterization of SR-coding genes in tomato (Solanum lycopersicum), a plant extensively used in HS studies. We identified 17 canonical SR and two SR-like genes. Several SR-coding genes show differential expression and altered splicing profiles in different organs as well as in response to HS. The transcriptional induction of five SR and one SR-like genes is partially dependent on the master regulator of HS response, HS transcription factor HsfA1a. Cis-elements in the promoters of these SR genes were predicted, which can be putatively recognized by HS-induced transcription factors. Further, transiently expressed SRs show reduced or steady-state protein levels in response to HS. Thus, the levels of SRs under HS are regulated by changes in transcription, alternative splicing and protein stability. We propose that the accumulation or reduction of SRs under HS can impact temperature-sensitive alternative splicing.
Metadaten
Author:Remus R. E. RosenkranzORCiD, Samia Bachiri, Stavros Vraggalas, Mario Keller, Stefan SimmORCiDGND, Enrico SchleiffORCiDGND, Sotirios FragkostefanakisORCiD
URN:urn:nbn:de:hebis:30:3-611272
DOI:https://doi.org/10.3389/fpls.2021.645689
ISSN:1664-462X
Parent Title (English):Frontiers in plant science
Publisher:Frontiers Media
Place of publication:Lausanne
Document Type:Article
Language:English
Date of Publication (online):2021/03/29
Date of first Publication:2021/03/29
Publishing Institution:Universit├Ątsbibliothek Johann Christian Senckenberg
Release Date:2021/06/09
Tag:Solanum lycopersicum; alternative splicing; heat stress; pre-mRNA; regulation
Volume:12
Issue:art. 645689
Page Number:18
First Page:1
Last Page:18
HeBIS-PPN:484661736
Institutes:Biowissenschaften
Dewey Decimal Classification:5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie
Sammlungen:Universit├Ątspublikationen
Open-Access-Publikationsfonds:Biowissenschaften
Licence (German):License LogoCreative Commons - Namensnennung 4.0