Herpes simplex virus type 1 neuronal infection triggers the disassembly of key structural components of dendritic spines

  • Herpes simplex virus type 1 (HSV-1) is a widespread neurotropic virus. Primary infection of HSV-1 in facial epithelium leads to retrograde axonal transport to the central nervous system (CNS) where it establishes latency. Under stressful conditions, the virus reactivates, and new progeny are transported anterogradely to the primary site of infection. During the late stages of neuronal infection, axonal damage can occur, however, the impact of HSV-1 infection on the morphology and functional integrity of neuronal dendrites during the early stages of infection is unknown. We previously demonstrated that acute HSV-1 infection in neuronal cell lines selectively enhances Arc protein expression - a major regulator of long-term synaptic plasticity and memory consolidation, known for being a protein-interaction hub in the postsynaptic dendritic compartment. Thus, HSV-1 induced Arc expression may alter the functionality of infected neurons and negatively impact dendritic spine dynamics. In this study we demonstrated that HSV-1 infection induces structural disassembly and functional deregulation in cultured cortical neurons, an altered glutamate response, Arc accumulation within the somata, and decreased expression of spine scaffolding-like proteins such as PSD-95, Drebrin and CaMKIIβ. However, whether these alterations are specific to the HSV-1 infection mechanism or reflect a secondary neurodegenerative process remains to be determined.
Author:Francisca Acuña-HinrichsenORCiD, Adriana Covarrubias-PintoORCiD, Yuta Ishizuka, María Francisca Stolzenbach, Carolina Martin, Paula Salazar, Maite A. Castro, Clive R. Bramham, Carola OtthORCiD
Parent Title (English):Frontiers in cellular neuroscience
Publisher:Frontiers Research Foundation
Place of publication:Lausanne
Document Type:Article
Date of Publication (online):2021/02/23
Date of first Publication:2021/02/23
Publishing Institution:Universitätsbibliothek Johann Christian Senckenberg
Release Date:2021/11/02
Tag:Arc protein; Herpes Simplex Virus Type 1 (HSV-1); dendritic spines; memory consolidation; neurodegeneration; neurotropic virus
Issue:art. 580717
Page Number:18
First Page:1
Last Page:18
These studies were funded by the following grants: Fondo Nacional de Desarrollo Científico y Tecnológico (FONDECYT) REGULAR 1180936 (CO), FONDECYT REGULAR 1150574 (CO), FONDECYT REGULAR 1151206 and 1191620 (MAC), The Research Council of Norway, Toppforsk grant/249951 (CRB) and CONICYT 21150756 (FA-H), CISNe-UACh, and DID-UACh.
Institutes:Biochemie, Chemie und Pharmazie
Dewey Decimal Classification:5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie
6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit
Licence (German):License LogoCreative Commons - Namensnennung 4.0