Subcellular localization of Fad1p in Saccharomyces cerevisiae: a choice at post-transcriptional level?

  • FAD synthase is the last enzyme in the pathway that converts riboflavin into FAD. In Saccharomyces cerevisiae, the gene encoding for FAD synthase is FAD1, from which a sole protein product (Fad1p) is expected to be generated. In this work, we showed that a natural Fad1p exists in yeast mitochondria and that, in its recombinant form, the protein is able, per se, to both enter mitochondria and to be destined to cytosol. Thus, we propose that FAD1 generates two echoforms—that is, two identical proteins addressed to different subcellular compartments. To shed light on the mechanism underlying the subcellular destination of Fad1p, the 3′ region of FAD1 mRNA was analyzed by 3′RACE experiments, which revealed the existence of (at least) two FAD1 transcripts with different 3′UTRs, the short one being 128 bp and the long one being 759 bp. Bioinformatic analysis on these 3′UTRs allowed us to predict the existence of a cis-acting mitochondrial localization motif, present in both the transcripts and, presumably, involved in protein targeting based on the 3′UTR context. Here, we propose that the long FAD1 transcript might be responsible for the generation of mitochondrial Fad1p echoform.
Author:Francesco Bruni, Teresa Anna Giancaspero, Igor-Mislav OrebORCiDGND, Maria Tolomeo, Piero Leone, Eckhard BolesORCiD, Marina Roberti, M. Caselle, Maria Barile
Parent Title (English):Life
Place of publication:Basel
Document Type:Article
Date of Publication (online):2021/09/14
Date of first Publication:2021/09/14
Publishing Institution:Universitätsbibliothek Johann Christian Senckenberg
Release Date:2022/03/02
Tag:FAD synthase; FAD1; Saccharomyces cerevisiae; mRNA; mitochondria localization; mitochondrial localization motif
Issue:9, art. 967
Page Number:18
First Page:1
Last Page:18
This work was supported by grants from MIUR PON-ricerca e competitività2007–2013 (project PON01_00937: 'Modelli sperimentali biotecnologici integrati per lo sviluppo e la selezione di molecole di interesse per la salute dell’uomo') and from Universitàdegli Studi di Bari 'Aldo Moro' (Progetti Competitivi 'Effetto di mutazioni di FLAD1 e di alterazioni dell’omeostasi delle flavine sullo stato redox e sulla biogenesi mitocondriale: uno studio integrato su fibroblasti umani') to M.B.
Dewey Decimal Classification:5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie
Licence (German):License LogoCreative Commons - Namensnennung 4.0