Club cell protein (CC)16 as potential lung injury marker in a porcine 72 h polytrauma model

  • Background: Polytrauma and respiratory tract damage after thoracic trauma cause about 25% of mortality among severely injured patients. Thoracic trauma can lead to the development of severe lung complications such as acute respiratory distress syndrome, and is, therefore, of great interest for monitoring in intensive care units (ICU). In recent years, club cell protein (CC)16 with its antioxidant properties has proven to be a potential outcome-related marker. In this study, we evaluated whether CC16 constitutes as a marker of lung damage in a porcine polytrauma model. Methods: In a 72 h ICU polytrauma pig model (thoracic trauma, tibial fracture, hemorrhagic shock, liver laceration), blood plasma samples (0, 3, 9, 24, 48, 72 h), BAL samples (72 h) and lung tissue (72 h) were collected. The trauma group (PT) was compared to a sham group. CC16 as a possible biomarker for lung injury in this model, and IL-8 concentrations as known indicator for ongoing inflammation during trauma were determined by ELISA. Histological analysis of ZO-1 and determination of total protein content were used to show barrier disruption and edema formation in lung tissue from the trauma group. Results: Systemic CC16 levels were significantly increased early after polytrauma compared vs. sham. After 72 h, CC16 concentration was significantly increased in lung tissue as well as in BAL in PT vs. sham. Similarly, IL-8 and total protein content in BAL were significantly increased in PT vs. sham. Evaluation of ZO-1 staining showed significantly lower signal intensity for polytrauma. Conclusion: The data confirm for the first time in a larger animal polytrauma model that lung damage was indicated by systemic and/or local CC16 response. Thus, early plasma and late BAL CC16 levels might be suitable to be used as markers of lung injury in this polytrauma model.

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Author:Johannes GrevenORCiDGND, Jan Tilmann VollrathORCiDGND, Felix Marius BläsiusORCiDGND, Zhizhen He, Eftychios BolierakisGND, Klemens HorstGND, Philipp StörmannORCiDGND, Aleksander J. NowakORCiD, Marija Simic, Ingo MarziORCiDGND, Frank HildebrandORCiDGND, Borna ReljaORCiDGND
Parent Title (English):European journal of trauma and emergency surgery
Publisher:Springer Medizin
Place of publication:Heidelberg
Document Type:Article
Date of Publication (online):2022/05/21
Date of first Publication:2022/05/21
Publishing Institution:Universitätsbibliothek Johann Christian Senckenberg
Release Date:2023/01/23
Tag:Biomarker; Inflammation; Lung failure; Tight junctions; Uteroglobin
Page Number:8
First Page:4719
Last Page:4726
Open Access funding enabled and organized by Projekt DEAL. The work was supported by grants from the DFG HI 820/5-1, DFG WU 820/2-1, and RE 3304/8-1.
Dewey Decimal Classification:6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit
Licence (German):License LogoCreative Commons - CC BY - Namensnennung 4.0 International