Die Gelchromatographie großer Partikel und ihre Grenzen
Limitation of particles size in gel chromatography
- The behaviour of molecules and particles of 2 - 400 nm radius in gel chromatography was investigated using sephadex, agarose, and pearl shaped cellulose gel of different porosity. Correlations between elution volumes and particle sizes are given in Fig. 1. We found that particles from ca. 400 nm diameter upwards were more or less irreversibly adsorbed depending on the particle size. So only 15% of rat liver mitochondria and 5% of E. coli bacteria could be eluted from the loose cellulose Cu3. It is assumed that this adsorption is due to the absence of Brownian motion of large particles, which therefore are more or less subject to the gravity and to adsorption forces of the gel.
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| Author: | Wolfram H. GerlichORCiDGND, Helmut DetermannGND, Theodor Wieland |
|---|---|
| URN: | urn:nbn:de:hebis:30:3-698561 |
| DOI: | https://doi.org/10.1515/znb-1970-1106 |
| ISSN: | 1865-7117 |
| ISSN: | 0932-0776 |
| Parent Title (German): | Zeitschrift für Naturforschung, B |
| Publisher: | Verlag der Zeitschrift für Naturforschung |
| Place of publication: | Tübingen |
| Document Type: | Article |
| Language: | German |
| Date of Publication (online): | 2014/06/02 |
| Year of first Publication: | 1970 |
| Publishing Institution: | Universitätsbibliothek Johann Christian Senckenberg |
| Release Date: | 2023/07/06 |
| Volume: | 25 |
| Issue: | 7 |
| Page Number: | 5 |
| First Page: | 1235 |
| Last Page: | 1239 |
| HeBIS-PPN: | 511913206 |
| Institutes: | Biochemie, Chemie und Pharmazie / Biochemie und Chemie |
| Dewey Decimal Classification: | 5 Naturwissenschaften und Mathematik / 54 Chemie / 540 Chemie und zugeordnete Wissenschaften |
| Sammlungen: | Universitätspublikationen |
| Licence (German): |  Creative Commons - Namensnennung-Nicht kommerziell-Keine Bearbeitung 3.0 |
