FAM134B-RHD protein clustering drives spontaneous budding of asymmetric membranes

  • Living cells constantly remodel the shape of their lipid membranes. In the endo-plasmic reticulum (ER), the reticulon homology domain (RHD) of the reticulophagy regulator 1 (RETR1/FAM134B) forms dense autophagic puncta that are associated with membrane removal by ER-phagy. In molecular dynamics (MD) simulations, we find that FAM134B-RHD spontaneously forms clusters, driven in part by curvature-mediated attraction. At a critical size, the FAM134B-RHD clusters induce the formation of membrane buds. The kinetics of budding depends sensitively on protein concentration and bilayer asymmetry. Our MD simulations shed light on the role of FAM134B-RHD in ER-phagy and show that membrane asymmetry can be used to modulate the kinetics barrier for membrane remodeling.

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Author:Marc SiggelORCiDGND, Ramachandra M. BhaskaraORCiD, Melanie K. MoesserORCiD, Ivan ĐikićORCiDGND, Gerhard HummerORCiD
Parent Title (English):bioRxiv
Document Type:Preprint
Date of Publication (online):2021/01/04
Date of first Publication:2021/01/04
Publishing Institution:Universitätsbibliothek Johann Christian Senckenberg
Release Date:2023/03/27
Page Number:16
Dewey Decimal Classification:5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie
Licence (German):License LogoCreative Commons - CC BY-ND - Namensnennung - Keine Bearbeitungen 4.0 International