Solid-phase-supported chemoenzymatic synthesis of a light-activatable tRNA derivative

  • Herein, we present a multi-cycle chemoenzymatic synthesis of modified RNA with simplified solid-phase handling to overcome size limitations of RNA synthesis. It combines the advantages of classical chemical solid-phase synthesis and enzymatic synthesis using magnetic streptavidin beads and biotinylated RNA. Successful introduction of light-controllable RNA nucleotides into the tRNAMet sequence was confirmed by gel electrophoresis and mass spectrometry. The methods tolerate modifications in the RNA phosphodiester backbone and allow introductions of photocaged and photoswitchable nucleotides as well as photocleavable strand breaks and fluorophores.
Author:Anja BlümlerGND, Harald SchwalbeORCiDGND, Alexander HeckelORCiDGND
Parent Title (English):Angewandte Chemie. International Edition
Place of publication:Weinheim
Document Type:Article
Date of Publication (online):2021/11/05
Date of first Publication:2021/11/05
Publishing Institution:Universitätsbibliothek Johann Christian Senckenberg
Release Date:2023/08/11
Tag:RNA synthesis; chemoenzymatic synthesis; ligases; light control; solid-phase synthesis
Issue:1, art. e202111613
Article Number:e202111613
Page Number:6
First Page:1
Last Page:6
This research was funded by the Deutsche Forschungsgemeinschaft (DFG) through GRK 1986—CliC (Complex Light Control). Work at BMRZ is supported by the state of Hesse. Open Access funding enabled and organized by Projekt DEAL.
Institutes:Biochemie, Chemie und Pharmazie
Wissenschaftliche Zentren und koordinierte Programme / Zentrum für Biomolekulare Magnetische Resonanz (BMRZ)
Dewey Decimal Classification:5 Naturwissenschaften und Mathematik / 54 Chemie / 540 Chemie und zugeordnete Wissenschaften
Licence (German):License LogoCreative Commons - CC BY - Namensnennung 4.0 International