Biosynthesis of fucoxanthin and diadinoxanthin and function of initial pathway genes in Phaeodactylum tricornutum
- The biosynthesis pathway to diadinoxanthin and fucoxanthin was elucidated in Phaeodactylum tricornutum by a combined approach involving metabolite analysis identification of gene function. For the initial steps leading to β-carotene, putative genes were selected from the genomic database and the function of several of them identified by genetic pathway complementation in Escherichia coli. They included genes encoding a phytoene synthase, a phytoene desaturase, a ζ-carotene desaturase, and a lycopene β-cyclase. Intermediates of the pathway beyond β-carotene, present in trace amounts, were separated by TLC and identified as violaxanthin and neoxanthin in the enriched fraction. Neoxanthin is a branching point for the synthesis of both diadinoxanthin and fucoxanthin and the mechanisms for their formation were proposed. A single isomerization of one of the allenic double bounds in neoxanthin yields diadinoxanhin. Two reactions, hydroxylation at C8 in combination with a keto-enol tautomerization and acetylation of the 3′-HO group results in the formation of fucoxanthin.
Verfasserangaben: | Michael Dambek, Ulrike Eilers, Jürgen BreitenbachGND, Sabine Steiger, Claudia BüchelORCiD, Gerhard SandmannORCiD |
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URN: | urn:nbn:de:hebis:30:3-255945 |
DOI: | https://doi.org/10.1093/jxb/ers211 |
ISSN: | 1460-2431 |
ISSN: | 0022-0957 |
Pubmed-Id: | https://pubmed.ncbi.nlm.nih.gov/22888128 |
Titel des übergeordneten Werkes (Englisch): | The journal of experimental botany |
Verlag: | Univ. Press |
Verlagsort: | Oxford |
Dokumentart: | Wissenschaftlicher Artikel |
Sprache: | Englisch |
Datum der Veröffentlichung (online): | 09.08.2012 |
Datum der Erstveröffentlichung: | 09.08.2012 |
Veröffentlichende Institution: | Universitätsbibliothek Johann Christian Senckenberg |
Datum der Freischaltung: | 15.08.2012 |
Freies Schlagwort / Tag: | Phaeodactylum tricornutum; carotenogenic genes; complementation; diadinoxanthin; fucoxanthin; genetic pathway; neoxanthin |
Jahrgang: | 63 |
Ausgabe / Heft: | 15 |
Seitenzahl: | 6 |
HeBIS-PPN: | 357876369 |
Institute: | Biowissenschaften / Biowissenschaften |
DDC-Klassifikation: | 5 Naturwissenschaften und Mathematik / 58 Pflanzen (Botanik) / 580 Pflanzen (Botanik) |
Sammlungen: | Universitätspublikationen |
Sammlung Biologie / Sondersammelgebiets-Volltexte | |
Lizenz (Deutsch): | ![]() |