Identification of potential novel interacting partners for coagulation factor xiii b (fxiii-b) subunit, a protein associated with a rare bleeding disorder
- Coagulation factor XIII (FXIII) is a plasma-circulating heterotetrameric pro-transglutaminase complex that is composed of two catalytic FXIII-A and two protective/regulatory FXIII-B subunits. FXIII acts by forming covalent cross-links within a preformed fibrin clots to prevent its premature fibrinolysis. The FXIII-A subunit is known to have pleiotropic roles outside coagulation, but the FXIII-B subunit is a relatively unexplored entity, both structurally as well as functionally. Its discovered roles so far are limited to that of the carrier/regulatory protein of its partner FXIII-A subunit. In the present study, we have explored the co-presence of protein excipients in commercial FXIII plasma concentrate FibrogamminP by combination of protein purification and mass spectrometry-based verification. Complement factor H was one of the co-excipients observed in this analysis. This was followed by performing pull down assays from plasma in order to detect the putative novel interacting partners for the FXIII-B subunit. Complement system proteins, like complement C3 and complement C1q, were amongst the proteins that were pulled down. The only protein that was observed in both experimental set ups was alpha-2-macroglobulin, which might therefore be a putative interacting partner of the FXIII/FXIII-B subunit. Future functional investigations will be needed to understand the physiological significance of this association.
Author: | Sneha Singh, Mohammad Suhail Akhter, Johannes Dodt, Peter Volkers, Andreas Reuter, Christoph Reinhart, Christoph Krettler, Johannes OldenburgORCiDGND, Arijit BiswasORCiD |
---|---|
URN: | urn:nbn:de:hebis:30:3-535640 |
DOI: | https://doi.org/10.3390/ijms20112682 |
ISSN: | 1422-0067 |
ISSN: | 1661-6596 |
Pubmed Id: | https://pubmed.ncbi.nlm.nih.gov/31159152 |
Parent Title (English): | International journal of molecular sciences |
Publisher: | Molecular Diversity Preservation International |
Place of publication: | Basel |
Document Type: | Article |
Language: | English |
Year of Completion: | 2019 |
Date of first Publication: | 2019/05/31 |
Publishing Institution: | Universitätsbibliothek Johann Christian Senckenberg |
Release Date: | 2020/05/20 |
Tag: | FXIII deficiency; Factor XIII; complement system; excipients; mass spectrometry; pleiotropy |
Volume: | 20 |
Issue: | 11, Art. 2682 |
Page Number: | 12 |
First Page: | 1 |
Last Page: | 12 |
Note: | This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited |
HeBIS-PPN: | 465082459 |
Institutes: | Biowissenschaften / Biowissenschaften |
Angeschlossene und kooperierende Institutionen / MPI für Biophysik | |
Dewey Decimal Classification: | 5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie |
Sammlungen: | Universitätspublikationen |
Licence (German): | Creative Commons - Namensnennung 4.0 |