D-Galacturonic acid reduction by S. cerevisiae for L-galactonate production from extracted sugar beet press pulp hydrolysate
- Pectin-rich residues are considered as promising feedstocks for sustainable production of platform chemicals. Enzymatic hydrolysis of extracted sugar beet press pulp (SBPP) releases the main constituent of pectin, d-galacturonic acid (d-GalA). Using engineered Saccharomyces cerevisiae, d-GalA is then reduced to l-galactonate (l-GalOA) with sorbitol as co-substrate. The current work addresses the combination of enzymatic hydrolysis of pectin in SBPP with a consecutive optimized biotransformation of the released d-GalA to l-GalOA in simple batch processes in stirred-tank bioreactors. Process conditions were first identified with synthetic media, where a product concentration of 9.9 g L-1 L-GalOA was obtained with a product selectivity of 99% (L-GalOA D-GalA-1) at pH 5 with 4% (w/v) sorbitol within 48 h. A very similar batch process performance with a product selectivity of 97% was achieved with potassium citrate buffered SBPP hydrolysate, demonstrating for the first time direct production of L-GalOA from hydrolyzed biomass using engineered S. cerevisiae. Combining the hydrolysis process of extracted SBPP and the biotransformation process with engineered S. cerevisiae paves the way towards repurposing pectin-rich residues as substrates for value-added chemicals.
Author: | Jacqueline WagnerORCiD, Dominik Schäfer, Nikolas von den Eichen, Christina Haimerl, Simon HarthGND, Igor-Mislav OrebORCiDGND, Philipp BenzORCiDGND, Dirk Weuster-BotzORCiDGND |
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URN: | urn:nbn:de:hebis:30:3-695145 |
DOI: | https://doi.org/10.1007/s00253-021-11433-5 |
ISSN: | 1432-0614 |
Parent Title (English): | Applied microbiology and biotechnology |
Publisher: | Springer |
Place of publication: | Berlin ; Heidelberg ; New York |
Document Type: | Article |
Language: | English |
Date of Publication (online): | 2021/07/16 |
Date of first Publication: | 2021/07/16 |
Publishing Institution: | Universitätsbibliothek Johann Christian Senckenberg |
Release Date: | 2023/06/15 |
Tag: | D-Galacturonicacid; Enzymatic hydrolysis; Extracted sugar beet press pulp; L-Galactonate; Pectin; Saccharomyces cerevisiae |
Volume: | 105 |
Issue: | 14-15 |
Page Number: | 13 |
First Page: | 5795 |
Last Page: | 5807 |
Note: | Open access funding enabled and organized by Projekt DEAL. This work was supported by the German Federal Ministry of Education and Research [grant number 031B0342A-C]. |
HeBIS-PPN: | 510029728 |
Institutes: | Biowissenschaften / Biowissenschaften |
Dewey Decimal Classification: | 5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie |
Sammlungen: | Universitätspublikationen |
Licence (German): | Creative Commons - CC BY - Namensnennung 4.0 International |