- Background: Bacterial meningitis is associated with high mortality and long-term neurological sequelae. Increasing the phagocytic activity of microglia could improve the resistance of the CNS against infections. We studied the influence of activin A, a member of the TGF-β family with known immunoregulatory and neuroprotective effects, on the functions of microglial cells in vitro.
Methods: Primary murine microglial cells were treated with activin A (0.13 ng/ml–13 μg/ml) alone or in combination with agonists of TLR2, 4, and 9. Phagocytosis of Escherichia coli K1 as well as release of TNF-α, IL-6, CXCL1, and NO was assessed.
Results: Activin A dose-dependently enhanced the phagocytosis of Escherichia coli K1 by microglial cells activated by agonists of TLR2, 4, and 9 without further increasing NO and proinflammatory cytokine release. Cell viability of microglial cells was not affected by activin A.
Conclusions: Priming of microglial cells with activin A could increase the elimination of bacteria in bacterial CNS infections. This preventive strategy could improve the resistance of the brain to infections, particularly in elderly and immunocompromised patients.
MetadatenAuthor: | Catharina Diesselberg, Sandra Ribes, Jana Seele, Annika Kaufmann, Sandra Redlich, Stephanie Bunkowski, Uwe-Karsten Hanisch, Uwe Michel, Roland Nau, Sandra Schütze |
---|
URN: | urn:nbn:de:hebis:30:3-466054 |
---|
DOI: | https://doi.org/10.1186/s12974-018-1209-2 |
---|
ISSN: | 1742-2094 |
---|
Pubmed Id: | https://pubmed.ncbi.nlm.nih.gov/29880000 |
---|
Parent Title (English): | Journal of neuroinflammation |
---|
Publisher: | BioMed Central |
---|
Place of publication: | London |
---|
Document Type: | Article |
---|
Language: | English |
---|
Year of Completion: | 2018 |
---|
Date of first Publication: | 2018/06/07 |
---|
Publishing Institution: | Universitätsbibliothek Johann Christian Senckenberg |
---|
Release Date: | 2018/06/14 |
---|
Tag: | Activin; Bacterial meningitis; CNS infection; E. coli; Innate immune system; Nitric oxide; Phagocytosis; Proinflammatory cytokines; TLR |
---|
Volume: | 15 |
---|
Issue: | 1, Art. 175 |
---|
Page Number: | 11 |
---|
First Page: | 1 |
---|
Last Page: | 11 |
---|
Note: | Open Access: This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
---|
HeBIS-PPN: | 434442526 |
---|
Institutes: | keine Angabe Fachbereich / Extern |
---|
Dewey Decimal Classification: | 6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit |
---|
Sammlungen: | Universitätspublikationen |
---|
Licence (German): | Creative Commons - Namensnennung 4.0 |
---|