Assessment of HIV-1 entry inhibitors by MLV/HIV-1 pseudotyped vectors

  • Background: Murine leukemia virus (MLV) vector particles can be pseudotyped with a truncated variant of the human immunodeficiency virus type 1 (HIV-1) envelope protein (Env) and selectively target gene transfer to human cells expressing both CD4 and an appropriate co-receptor. Vector transduction mimics the HIV-1 entry process and is therefore a safe tool to study HIV-1 entry. Results: Using FLY cells, which express the MLV gag and pol genes, we generated stable producer cell lines that express the HIV-1 envelope gene and a retroviral vector genome encoding the green fluorescent protein (GFP). The BH10 or 89.6 P HIV-1 Env was expressed from a bicistronic vector which allowed the rapid selection of stable cell lines. A codon-usage-optimized synthetic env gene permitted high, Rev-independent Env expression. Vectors generated by these producer cells displayed different sensitivity to entry inhibitors. Conclusion: These data illustrate that MLV/HIV-1 vectors are a valuable screening system for entry inhibitors or neutralizing antisera generated by vaccines.

Download full text files

Export metadata

Additional Services

Share in Twitter Search Google Scholar
Author:Sandra Siegert, Sonja Thaler, Ralf Wagner, Barbara SchnierleORCiDGND
Parent Title (German):AIDS Research and Therapy
Document Type:Article
Year of Completion:2005
Date of first Publication:2005/09/12
Publishing Institution:Universitätsbibliothek Johann Christian Senckenberg
Release Date:2006/05/08
Tag:HIV-1; MLV/HIV-1; pseudotyped vectors
Page Number:6
© 2005 Siegert et al; licensee BioMed Central Ltd.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (,
which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Source:AIDS Res Ther. 2005; 2: 7.
Institutes:Angeschlossene und kooperierende Institutionen / Georg-Speyer-Haus
Dewey Decimal Classification:6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit
Licence (German):License LogoCreative Commons - Namensnennung 2.0