Phage display selection of HIV specific conserved mimotopes with IgG from long-term non-progressors

  • Poster presentation Background The aim of this study is to identify conserved epitopes of HIV-1 neutralizing antibodies in polyclonal plasma from LTNP to finally derive vaccine candidates. Materials and methods The presence of neutralizing antibodies in 9 LTNP sera was proved by in vitro neutralization assays. Phage displayed peptide libraries were screened with LTNP IgG. HIV-specific mimotopes were analyzed for homology to the gp120 structure by a software (3DEX) especially developed for this purpose. Mice were immunized with interesting phages and their sera were analyzed for neutralizing activities against HIV-1. Results After biopannings, between 19% and 75% HIV-specific phage clones were identified by ELISA. Mimotope sequences were identified and could be aligned by 3DEX to linear or conformational epitopes on gp120. A peptide specific immune response was detected in sera of immunized mice. The first mice sera analyzed showed neutralizing activities against HIV-1. Conclusion Mimotopes could be selected from LTNP sera that represent conformational epitopes on gp120. Those ones inducing neutralizing antibodies upon immunization potentially are suited to derive vaccine candidates.

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Author:Michael Humber, Sascha Antoni, Andreas Schreiber, Berta Rodes, Vicente Soriano, Matthias Dittmar, Ursula Dietrich
Parent Title (English):Retrovirology
Publisher:BioMed Central
Place of publication:London
Document Type:Article
Year of Completion:2005
Date of first Publication:2005/12/08
Publishing Institution:Universitätsbibliothek Johann Christian Senckenberg
Release Date:2007/05/22
Issue:(Suppl 1):P29
Page Number:1
First Page:1
Last Page:1
licensee BioMed Central Ltd.
Source:Retrovirology 2005, 2(Suppl 1):P29. - 2005 International Meeting of The Institute of Human Virology, Baltimore, USA. 29 August – 2 September 2005. - doi:10.1186/1742-4690-2-S1-P29
Institutes:Angeschlossene und kooperierende Institutionen / Georg-Speyer-Haus
Dewey Decimal Classification:6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit
Licence (German):License LogoDeutsches Urheberrecht