Chemoresistance acquisition induces a global shift of expression of aniogenesis-associated genes and increased pro-angogenic activity in neuroblastoma cells

  • Background Chemoresistance acquisition may influence cancer cell biology. Here, bioinformatics analysis of gene expression data was used to identify chemoresistance-associated changes in neuroblastoma biology. Results Bioinformatics analysis of gene expression data revealed that expression of angiogenesis-associated genes significantly differs between chemosensitive and chemoresistant neuroblastoma cells. A subsequent systematic analysis of a panel of 14 chemosensitive and chemoresistant neuroblastoma cell lines in vitro and in animal experiments indicated a consistent shift to a more pro-angiogenic phenotype in chemoresistant neuroblastoma cells. The molecular mechanims underlying increased pro-angiogenic activity of neuroblastoma cells are individual and differ between the investigated chemoresistant cell lines. Treatment of animals carrying doxorubicin-resistant neuroblastoma xenografts with doxorubicin, a cytotoxic drug known to exert anti-angiogenic activity, resulted in decreased tumour vessel formation and growth indicating chemoresistance-associated enhanced pro-angiogenic activity to be relevant for tumour progression and to represent a potential therapeutic target. Conclusions A bioinformatics approach allowed to identify a relevant chemoresistance-associated shift in neuroblastoma cell biology. The chemoresistance-associated enhanced pro-angiogenic activity observed in neuroblastoma cells is relevant for tumour progression and represents a potential therapeutic target.
Metadaten
Author:Martin MichaelisORCiDGND, Denise Klassert, Susanne Barth, Tatyana Suhan, Rainer Breitling, Bernd Mayer, Nora Hinsch, Hans Wilhelm DoerrGND, Jindrich Cinatl, Jindrich Cinatl
URN:urn:nbn:de:hebis:30-71518
DOI:https://doi.org/10.1186/1476-4598-8-80
ISSN:1476-4598
Pubmed Id:https://pubmed.ncbi.nlm.nih.gov/19788758
Parent Title (English):Molecular cancer
Publisher:Biomed Central
Place of publication:London
Document Type:Article
Language:English
Date of Publication (online):2009/10/27
Date of first Publication:2009/09/29
Publishing Institution:Universitätsbibliothek Johann Christian Senckenberg
Release Date:2009/10/27
Volume:8
Issue:Art. 80
Page Number:14
First Page:1
Last Page:14
Note:
© 2009 Michaelis et al. , licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited
Source:Molecular Cancer 2009, 8:80 ; doi:10.1186/1476-4598-8-80 ; http://www.molecular-cancer.com/content/8/1/80
HeBIS-PPN:219616558
Institutes:Medizin / Medizin
Dewey Decimal Classification:6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit
Licence (German):License LogoCreative Commons - Namensnennung 2.0