Real-Time analysis and visualization for single-molecule based super-resolution microscopy

  • Accurate multidimensional localization of isolated fluorescent emitters is a time consuming process in single-molecule based super-resolution microscopy. We demonstrate a functional method for real-time reconstruction with automatic feedback control, without compromising the localization accuracy. Compatible with high frame rates of EM-CCD cameras, it relies on a wavelet segmentation algorithm, together with a mix of CPU/GPU implementation. A combination with Gaussian fitting allows direct access to 3D localization. Automatic feedback control ensures optimal molecule density throughout the acquisition process. With this method, we significantly improve the efficiency and feasibility of localization-based super-resolution microscopy.

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Metadaten
Author:Adel Kechkar, Deepak Nair, Mike HeilemannORCiDGND, Daniel Choquet, Jean-Baptiste SibaritaORCiD
URN:urn:nbn:de:hebis:30:3-294966
DOI:https://doi.org/10.1371/journal.pone.0062918
ISSN:1932-6203
Parent Title (English):PLoS One
Publisher:PLoS [u.a.]
Place of publication:Lawrence, Kan.
Document Type:Article
Language:English
Date of first Publication:2013/04/30
Publishing Institution:Universit├Ątsbibliothek Johann Christian Senckenberg
Release Date:2013/05/03
Volume:8
Issue:(4): e62918
Page Number:10
HeBIS-PPN:338741801
Institutes:Biochemie, Chemie und Pharmazie / Biochemie und Chemie
Dewey Decimal Classification:5 Naturwissenschaften und Mathematik / 54 Chemie / 540 Chemie und zugeordnete Wissenschaften
Sammlungen:Universit├Ątspublikationen
Licence (German):License LogoCreative Commons - Namensnennung 3.0