Molecular characterization of methanogenic N(5)-methyl-tetrahydromethanopterin : coenzyme M methyltransferase

  • Methanogenic archaea share one ion gradient forming reaction in their energy metabolism catalyzed by the membrane-spanning multisubunit complex N5-methyl-tetrahydromethanopterin: coenzyme M methyltransferase (MtrABCDEFGH or simply Mtr). In this reaction the methyl group transfer from methyl-tetrahydromethanopterin to coenzyme M mediated by cobalamin is coupled with the vectorial translocation of Na+ across the cytoplasmic membrane. No detailed structural and mechanistic data are reported about this process. In the present work we describe a procedure to provide a highly pure and homogenous Mtr complex on the basis of a selective removal of the only soluble subunit MtrH with the membrane perturbing agent dimethyl maleic anhydride and a subsequent two-step chromatographic purification. A molecular mass determination of the Mtr complex by laser induced liquid bead ion desorption mass spectrometry (LILBID-MS) and size exclusion chromatography coupled with multi-angle light scattering (SEC-MALS) resulted in a (MtrABCDEFG)3 heterotrimeric complex of ca. 430 kDa with both techniques. Taking into account that the membrane protein complex contains various firmly bound small molecules, predominantly detergent molecules, the stoichiometry of the subunits is most likely 1:1. A schematic model for the subunit arrangement within the MtrABCDEFG protomer was deduced from the mass of Mtr subcomplexes obtained by harsh IR-laser LILBID-MS.

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Metadaten
Author:Vikrant Upadhyay, Katharina Ceh, Franz Tumulka, Rupert Abele, Jan Hoffmann, Julian David Langer, Seigo Shima, Ulrich Ermler
URN:urn:nbn:de:hebis:30:3-447143
DOI:https://doi.org/10.1016/j.bbamem.2016.06.011
ISSN:1879-2642
ISSN:0005-2736
Pubmed Id:https://pubmed.ncbi.nlm.nih.gov/27342374
Parent Title (English):Biochimica et biophysica acta
Publisher:Elsevier
Place of publication:Amsterdam
Document Type:Article
Language:English
Year of Completion:2016
Date of first Publication:2016/06/21
Publishing Institution:Universitätsbibliothek Johann Christian Senckenberg
Release Date:2018/01/09
Tag:Dimethyl maleic anhydride; LILBID-MS; Membrane protein complex; Methanogenesis; N5 -methyl-tetrahydromethanopterin: coenzyme M methyltransferase; SEC-MALS
Volume:1858
Issue:9
Page Number:5
First Page:2140
Last Page:2144
Note:
Under an Elsevier user license
HeBIS-PPN:426711793
Institutes:Biochemie, Chemie und Pharmazie / Biochemie und Chemie
Angeschlossene und kooperierende Institutionen / MPI für Biophysik
Dewey Decimal Classification:5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie
Sammlungen:Universitätspublikationen
Licence (German):License LogoArchivex. zur Lesesaalplatznutzung § 52b UrhG