A 192-heme electron transfer network in the hydrazine dehydrogenase complex

  • Anaerobic ammonium oxidation (anammox) is a major process in the biogeochemical nitrogen cycle in which nitrite and ammonium are converted to dinitrogen gas and water through the highly reactive intermediate hydrazine. So far, it is unknown how anammox organisms convert the toxic hydrazine into nitrogen and harvest the extremely low potential electrons (−750 mV) released in this process. We report the crystal structure and cryo electron microscopy structures of the responsible enzyme, hydrazine dehydrogenase, which is a 1.7 MDa multiprotein complex containing an extended electron transfer network of 192 heme groups spanning the entire complex. This unique molecular arrangement suggests a way in which the protein stores and releases the electrons obtained from hydrazine conversion, the final step in the globally important anammox process.

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Author:Mohd Akram, Andreas Dietl, Ulrike Mersdorf, Simone Prinz, Wouter J. Maalcke, Jan Keltjens, Christina Ferousi, Naomi M. de Almeida, Joachim Reimann, Boran Kartal, Michael S. M. Jetten, Kristian Kurt Parey, Thomas Reinier Maxim Barends
URN:urn:nbn:de:hebis:30:3-500704
DOI:https://doi.org/10.1126/sciadv.aav4310
ISSN:2375-2548
Pubmed Id:https://pubmed.ncbi.nlm.nih.gov/31001586
Parent Title (English):Science advances
Publisher:Assoc.
Place of publication:Washington, DC [u. a.]
Document Type:Article
Language:English
Year of Completion:2019
Date of first Publication:2019/04/17
Publishing Institution:Universitätsbibliothek Johann Christian Senckenberg
Release Date:2019/05/06
Volume:5
Issue:4, eaav4310
Page Number:8
First Page:1
Last Page:7
Note:
Copyright © 2019 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC).
This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial license, which permits use, distribution, and reproduction in any medium, so long as the resultant use is not for commercial advantage and provided the original work is properly cited.
HeBIS-PPN:450768538
Institutes:Biowissenschaften / Biowissenschaften
Dewey Decimal Classification:5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie
Sammlungen:Universitätspublikationen
Licence (English):License LogoCreative Commons - Namensnennung-Nicht kommerziell 4.0