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Heterologously expressed genes require adaptation to the host organism to ensure adequate levels of protein synthesis, which is typically approached by replacing codons by the target organism’s preferred codons. In view of frequently encountered suboptimal outcomes we introduce the codon-specific elongation model (COSEM) as an alternative concept. COSEM simulates ribosome dynamics during mRNA translation and informs about protein synthesis rates per mRNA in an organism- and context-dependent way. Protein synthesis rates from COSEM are integrated with further relevant covariates such as translation accuracy into a protein expression score that we use for codon optimization. The scoring algorithm further enables fine-tuning of protein expression including deoptimization and is implemented in the software OCTOPOS. The protein expression score produces competitive predictions on proteomic data from prokaryotic, eukaryotic, and human expression systems. In addition, we optimized and tested heterologous expression of manA and ova genes in Salmonella enterica serovar Typhimurium. Superiority over standard methodology was demonstrated by a threefold increase in protein yield compared to wildtype and commercially optimized sequences.
In Auszügen rezipiert – Bemerkungen zu der Übersetzung von Max Frischs Tagebüchern ins Rumänische
(2010)
Romanian readers interested in Max Frisch’s literary diaries can find the Romanian translation of some heterogeneous notes or groups of notes, dispersed in different periodicals from 1972 to 2001, and the volume Journal (1984) including a selection of fragments from the diaries Tagebuch 1946-1949 and Tagebuch 1966-1971 translated by Corina Jiva. The present article emphasizes favorite as well as excluded thematic fields while making the selection and pinpoints the translator’s role as an authority endowed with preventive censorship.
During the co-translational assembly of protein complexes, a fully synthesized subunit engages with the nascent chain of a newly synthesized interaction partner. Such events are thought to contribute to productive assembly, but their exact physiological relevance remains underexplored. Here, we examine structural motifs contained in nucleoporins for their potential to facilitate co-translational assembly. We experimentally test candidate structural motifs and identify several previously unknown co-translational interactions. We demonstrate by selective ribosome profiling that domain invasion motifs of beta-propellers, coiled-coils, and short linear motifs may act as co-translational assembly domains. Such motifs are often contained in proteins that are members of multiple complexes (moonlighters) and engage with closely related paralogs. Surprisingly, moonlighters and paralogs assemble co-translationally in only some but not all of the relevant biogenesis pathways. Our results highlight the regulatory complexity of assembly pathways.
Die Übersetzung der Kashinawa-Mythen in Theodor Koch-Grünbergs "Indianermärchen aus Südamerika"
(2019)
Neben seinen ethnologischen und linguistischen Studien zu indigenen Kulturen der Amazonasregion beschäftigte sich der deutsche Brasilienforscher Theodor Koch-Grünberg auch mit indigenen Mythen und hatte klare Vorstellungen davon, wie diese mündlich überlieferten Erzählungen dokumentiert und übersetzt werden sollten. Modellcharakter hatte für ihn die interlineare Übersetzung des brasilianischen Historikers João Capistrano de Abreu von Texten aus dem Kashinawa, einer Panosprache, die noch heute im brasilianisch-peruanischen Grenzgebiet von Mitgliedern der ethnischen Gruppe gesprochen wird. Capistranos Textsammlung "Rã-txa hu-ni ku-ĩ" wurde 1914 in Brasilien veröffentlicht. Bereits 1920 erfolgte die Publikation der "Indianermärchen aus Südamerika" durch Koch-Grünberg, wobei von den 117 ins Deutsche übertragenen Geschichten 13 aus Capistranos Werk stammten. In diesem Beitrag soll anhand ausgewählter Textbeispiele die Übersetzung Koch-Grünbergs aus dem Kashinawa ins Deutsche diskutiert werden. Grundlage war dabei die transkribierte Textsammlung in Originalsprache mit einer Wort-für-Wort-Übersetzung ins Portugiesische sowie einem angehängten Glossar und einem Grammatiksketch. In Koch-Grünbergs eigenen Aufzeichnungen wird zusätzlich deutlich, dass es sich um einen multiplen Übersetzungsprozess handelt: von einem mündlich präsentierten narrativen Diskurs mit performativen Elementen in Schriftsprache, von einer indigenen Sprache Südamerikas in eine typologisch weit entfernte europäische und von einer vollkommen unbekannten Kultur in eine für den brasilianischen wie den deutschen Leser nachvollziehbare.
Als eines der "treibenden Kräfte" des Internets kann das populäre Videoportal YouTube angesehen werden, das täglich von Millionen von Internetnutzern besucht wird. Es ist nicht nur ein Online-Feld, in dem Menschen aus aller Welt Videos aus den unterschiedlichsten Weltbereichen oder ihrer Interessen teilen, sondern auch aus sprachlicher Sicht handelt es sich um einen faszinierenden Ort. Dies spiegelt sich in der sog. YouTube-Sprache wider, die das Hauptthema dieses Beitrags ist. Das Hauptziel des Beitrags ist es dabei, auf das Phänomen "YouTube-Sprache" aufmerksam zu machen und sie in Bezug auf die Merkmale der Internetsprache zu behandeln. Das zusammenhängende Ziel ist es, durch konkrete Beispiele darauf hinzuweisen, wie sich diese "Sprache" im Deutschen, bzw. im deutschsprachigen YouTube-Bereich, manifestiert. Ein weiteres Ziel besteht in der Klärung einiger angeführter Beispiele der YouTube-Sprache, d.h. der Wörter, bzw. Wortverbindungen, die zum gesamten Charakter dieser Sprache beitragen.
The Romanian folk poetry, especially the Romanian folk ballad raised the interest of German translators in the 19th century, who proved the size of their talent by translating the ballads in German and by popularizing them among the German readers in the country and abroad. The paper focuses on aspects of translation motifs of five German authors (W. von Kotzebue, C.F.W. Rudow, A. Franken, A. Forstenheim, Carmen Sylva) taking into account their life experiences, professional commitments and friendship for the Romanians. The study aims to highlight the principles and difficulties of translation as a result of the authors’ wish to translate the original text as accurately as possible and as close as possible to the spirit of the Romanian folk poetry.
The present study intends to analyze the literary personality of the German-language writer and translator Alfred Margul-Sperber and his approach to Romanian folk poetry and Romanian folk ballads. Alfred Margul-Sperber is known as a classical German-language poet from Romania, as a discoverer and supporter of literary talent, but also as a brilliant translator. Impressions from his childhood and life experiences contributed to Alfred MargulSperber’s interest for Romanian poets and especially for Romanian folk poetry, which left its own mark on Sperber’s literary creation. The author was fascinated by its plurality of forms, its richness of rhymes, images and sounds. The masterpiece of Romanian folk poetry, “Mioriţa/The little Ewe”-ballad raised the interest of the multilingual translator who proved his outstanding talent by translating the original text into German as accurately as possible and remarkably close to the spirit of the Romanian folk poetry, thus popularizing it among the German readers at home and abroad.
Translation is a complex activity which does not involve merely the translation of the given text from the source language to the target one, but also means observing many other aspects which need to be preserved in translation. What is more, every type of text has different requirements, depending on stylistic norms, cultural aspects etc. This paper focuses on popular science literature for children. In this area, the precision of the translation is not the sole criterion, but also the degree of equivalence in the comprehension of the source and target language texts plays a substantial role. Taking the example of a popular science book entitled "Tiere im Hohen Norden" by Hensel and Thiemeyer (1994) and its translation into Czech entitled "Severská zvířata", based on Göpferich's dimensions of comprehension, it is examined how the comprehension is preserved in the process of translation. Furthermore, it explores how and where modifications occur and their impact on the resulting translation.
In this paper, I will address the issue of translation as a critique of autochthony that emerges in the context of Fritz Mauthner's linguistic scepticism. Translation, for Mauthner, becomes a privileged prism through which to consider identity and belonging, as well as a way of understanding uprootedness, since language is a continuous product of borrowing, bastardization, stratification, and contingency. According to Mauthner, languages are not possession, but borrowing; not purity, but contagion; not an abstract crystallization, but transit. Therefore, love of the mother tongue - the only way to conceive patriotism - is not a physical connection with the land, roots, or nation, but a refuge, an always precarious 'Heimat' (home).
Este trabalho tem como objetivo discutir as noções de apropriação pela via da tradução a partir da análise de oito excertos da Preparação à primeira parte da obra "Die Kunst das Clavier zu spielen" de Friedrich Wilhelm Marpurg. A Preparação desta obra compõe-se de 23 parágrafos, dos quais oito refletem ideias apresentadas numa obra anterior, a saber, "L'Art de toucher le clavecin" (1716), de François Couperin. Para este trabalho será considerado o reflexo dessas ideias pela via da tradução. Os trechos traduzidos aparecem ora aumentados, ora reduzidos, ou então são suprimidos. Esta dinâmica de tradução é afirmada por Marpurg no prefácio de duas edições anteriores a 1762: as edições de 1750 e 1751. Investigamos, nas discussões sobre apropriação, o que teria levado Marpurg a abandonar a reverência a Couperin nos prefácios de edições posteriores, o que contribuiu para que essa apropriação - ocorrida também, mas não apenas por meio da tradução - pudesse ser relacionada, a princípio, à autoria. Nesse sentido, apoiados em obras científicas sobre tradução e apropriação, discutimos a noção de tradução, apropriação e autoria, traçando um diálogo com duas áreas dos Estudos da Tradução: análise comparativa e tradução comentada.