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Im Beitrag wird auf aktuelle Ergebnisse der Forschung zum mentalen Lexikon eingegangen. Das mentale Lexikon wird dabei aus der Sicht der Netzwerktheorie untersucht. Der Netzwerkcharakter des mentalen Lexikons hat zur Folge, dass die gelernten Wörter nicht voneinander unabhängig existieren: Sie sind miteinander verknüpft. Diese Verknüpfungen sind nicht gleich stark; zudem ist die Richtung dieser Verknüpfung von großer Bedeutung. Assoziationstests in mehreren Sprachen zeigen, dass dasselbe Wort in verschiedenen Sprachen verschiedene Verknüpfungen hat bzw. dass die Stärke der Verknüpfungen zwischen zwei beliebigen Wörtern von Sprache zu Sprache variiert. Beim Fremdsprachenerwerb und Fremdsprachenunterricht werden diese Unterschiede kaum berücksichtigt. Der vorliegende Beitrag analysiert das mentale Lexikon aus der Perspektive der Netzwerke. Dabei wird das Projekt ConnectYourMind vorgestellt, das in mehreren Sprachen Assoziationsdaten sammelt.
Pirsig und die Deutschen
(2019)
Der US-Autor Robert M. Pirsig hat zwei philosophische Romane über die Qualität geschrieben: "Zen und die Kunst, ein Motorrad zu warten: Ein Versuch über Werte" (1974) und "Lila – Oder ein Versuch über Moral" (1991). Beide sind literarische Experimente, die mit der deutschen Literatur und Philosophie zwar subtil, aber eng verbunden sind. Der Beitrag möchte diese Verbindungen aufzeigen und charakterisieren.
In der Studie werden die Gedichte von Ludwig Eichrodt (1827-1892), über Süleyman den Prächtigen, Nikolaus (Miklós) Zrínyi (1508-1566) und Theodor Körner (1791-1813) im Kontext des Schulunterrichtes, der Mediengeschichte und der Körner-Rezeption vorgestellt. Die Gedichte des Deutschen Knabenbuch sind scheinbar für Schulknaben gedacht. Sie zeigen aber eine mehrdeutige ironische Sprache und eine zivilisationskritische Attitüde, die die Gedichte untauglich für den Schulunterricht macht, weil sie den Schulkanon und die Werte des Unterrichts in Frage stellen. In der Geschichte der Körner/Zrínyi-Rezeption weichen die Texte ebenfalls von dem Mainstream ab, weil in der Körner-Rezeption selten ironische Stimmen zu hören waren.
We propose a variation of online paging in two-level memory systems where pages in the fast cache get modified and therefore have to be explicitly written back to the slow memory upon evictions. For increased performance, up to alpha arbitrary pages can be moved from the cache to the slow memory within a single joint eviction, whereas fetching pages from the slow memory is still performed on a one-by-one basis. The main objective in this new alpha-paging scenario is to bound the number of evictions. After providing experimental evidence that alpha-paging can adequately model flash-memory devices in the context of translation layers we turn to the theoretical connections between alpha-paging and standard paging. We give lower bounds for deterministic and randomized alpha-paging algorithms. For deterministic algorithms, we show that an adaptation of LRU is strongly competitive, while for the randomized case we show that by adapting the classical Mark algorithm we get an algorithm with a competitive ratio larger than the lower bound by a multiplicative factor of approximately 1.7.
Intra-arterial (IA) chemotherapy for curative treatment of head and neck cancer experienced a revival in the last decade. Mainly, it was used in concurrent combination with radiation in organ-preserving settings. The modern method of transfemoral approach for catheterisation, superselective perfusion of the tumour-feeding vessel, and high-dose (150 mg m−2) administration of cisplatin with parallel systemic neutralisation with sodium thiosulphate (9 g m−2) made preoperative usage feasible. The present paper presents the results of a pilot study on a population of 52 patients with resectable stage 1–4 carcinomas of the oral cavity and the oropharynx, who were treated with one cycle of preoperative IA chemotherapy executed as mentioned above and radical surgery. There have been no interventional complications of IA chemotherapy, and acute side effects have been low. One tracheotomy had to be carried out due to swelling. The overall clinical local response has been 69%. There was no interference with surgery, which was carried out 3–4 weeks later. Pathological complete remission was assessed in 25%. The mean observation time was 3 years. A 3-year overall and disease-free survival was 82 and 69%, respectively, and at 5 years 77 and 59%, respectively. Survival results were compared to a treatment-dependent prognosis index for the same population. As a conclusion, it can be stated that IA high-dose chemotherapy with cisplatin and systemic neutralisation in a neoadjuvant setting should be considered a feasible, safe, and effective treatment modality for resectable oral and oropharyngeal cancer. The low toxicity of this local chemotherapy recommends usage especially in stage 1–2 patients. The potential of survival benefit as indicated by the comparison to the prognosis index should be controlled in a randomised study.
Expeditions of Ron Brechlin, Viktor Synjaev, Mildred Márquez, Juan Machado, Oleg Romanov and other colleagues over the last five years in Colombia, Ecuador and Bolivia have resulted in significant collections of Pterophoridae Zeller, 1841. The article describes five new species: Singularia brechlini Kovtunovich & Ustjuzhanin sp. nov., Singularia sinjaevi Kovtunovich & Ustjuzhanin sp. nov., Singularia guajiro Kovtunovich & Ustjuzhanin sp. nov., Singularia tolima Kovtunovich & Ustjuzhanin sp. nov. and Singularia lesya Kovtunovich & Ustjuzhanin sp. nov.
Epithelial cells enable essential physiological functions, including absorption, morphogenesis, secretion, and transport. To execute these functions, epithelial cells often form three-dimensional shapes that include curved sheets of cells surrounding a pressurized fluid-filled lumen. These three-dimensional tissues (called domes) are essential for organ function, but when they are not working properly, developmental defects, inflammation, and cancer can ensue. Recently, it has been shown that the cells that form domes show active superelasticity on micropatterned plates.
We show here that the immortalized renal proximal tubule epithelial cell line, LLC-PK1, stereotypically forms tubules in 10 days. Tubule formation takes place in 4 stages. When cells are plated on a culture dish, they form a monolayer on the 1st day; on the 3rd day, three-dimensional structures are formed, called domes; and after the 4.5th day, these domes start fusing to begin the transition stage and transit to the tubule stage. At the end of the 10th day, differentiated, elongated, and matured tubes form (Figure 3.1). Therefore, tubule formation is a self-organized, stereotypic morphogenetic program under long-term, unperturbed tissue culture conditions.
We propose that tubulogenesis is a two-step process in proximal tubules by doming and wrapping. The process begins with dome formation, and as the cell layers come together in the transition stage at the edge of the dome, this leads to the formation of the lumen of the eventual tubule. We also found that F-actin provides the mechanical strength during the formation of these three-dimensional structures during tubule formation. To better understand this 4-step process on a molecular level, we performed proteomics of tubule formation to identify the different proteins that play a significant role in proximal tubule development. Importantly, we identified proximal tubule markers like synaptopondin, angiotensin 1-10, collectrin, polycystin 1, and polycystin 2. These proteins play an important role in renal tube formation and differentiation.
Cell division is carried out by highly conserved cyclin-CDK complexes, which phosphorylate various cellular components. Cyclin-CDKs act differently depending on the cell cycle phase and work cooperatively to create DNA replication and cytokinesis. Therefore, we identified that cyclin-B1, marker of proliferation Ki-67, the RAD51 recombinase, and proliferating cell nuclear antigen (PNCA) are upregulated in the monolayer stage, and the expression decreases as tubule formation takes place. The proximal tubule reabsorbs 60-65% of the glomerulus filtrate. Therefore, it requires a lot of energy generated by using the fatty acid oxidation (FAO) pathway. In our model, we found FAO expression is higher than that of the other metabolic pathways.
We found expression of an intricate protein network in mitochondria, which we interpret as a sign of mitochondrial homeostasis being vital for the FAO pathway to work. Furthermore, we also identified different types of transporters at each stage of proximal tubule formation, and we could recognize different cytoskeletal components playing a significant role in each stage of proximal tubule formation, for instance, at the monolayer stage, vimentin expression is high, and its expression is reduced as tubules form. Hence, this 2D system, at this step of characterization, seems suitable to use to study differential transport protein expression and how this might relate to physiological functions and syndromes.
Next, we inhibited different transporters using specific inhibitors and analyzed the effect on dome and tubule formation. We identified that Na+/K+ ATPase and vacuolar H+ ATPase play a significant role in the process of epithelial dynamics. Digoxin (a Na+/K+ ATPase inhibitor) treatment inhibits dome and tubule formation. Bafilomycin (a v-ATPase inhibitor) treatment demonstrated a delay in dome and tube formation. Therefore, this study shows that this 2D proximal tubule novel system can be used for screening of pharmacological leads in the context of specific aspects of kidney physiology.
Despite the recent success in growing kidney organoids, they are not well suited to investigate various pathophysiological conditions in vitro for several reasons: They grow in 3D and form a tissue that later needs to be dissected/cleared and stained to investigate pathophysiological changes. Moreover, organoids require complex and expensive protocols for generation and are challenging to use in screening approaches. Therefore, we set out to demonstrate feasibility for our 2D system using normal renal epithelial cells, which are the origin of various pathological conditions, to study pathophysiological conditions.
Advertising arbitrage
(2020)
Arbitrageurs with a short investment horizon gain from accelerating price discovery by advertising their private information. However, advertising many assets may overload investors' attention, reducing the number of informed traders per asset and slowing price discovery. So arbitrageurs optimally concentrate advertising on just a few assets, which they overweight in their portfolios. Unlike classic insiders, advertisers prefer assets with the least noise trading. If several arbitrageurs share information about the same assets, inefficient equilibria can arise, where investors' attention is overloaded and substantial mispricing persists. When they do not share, the overloading of investors' attention is maximal.
Advertising arbitrage
(2014)
Speculators often advertise arbitrage opportunities in order to persuade other investors and thus accelerate the correction of mispricing. We show that in order to minimize the risk and the cost of arbitrage an investor who identifies several mispriced assets optimally advertises only one of them, and overweights it in his portfolio; a risk-neutral arbitrageur invests only in this asset. The choice of the asset to be advertised depends not only on mispricing but also on its "advertisability" and accuracy of future news about it. When several arbitrageurs identify the same arbitrage opportunities, their decisions are strategic complements: they invest in the same asset and advertise it. Then, multiple equilibria may arise, some of which inefficient: arbitrageurs may correct small mispricings while failing to eliminate large ones. Finally, prices react more strongly to the ads of arbitrageurs with a successful track record, and reputation-building induces high-skill arbitrageurs to advertise more than others.
Im vorliegenden Beitrag werden die Ergebnisse der kontrastiven Forschung von phraseologischen Neologismen im Deutschen und Ukrainischen gezeigt. Das Ziel dieses Beitrags besteht darin, die phraseologischen Neologismen, die in der Corona-Pandemie in Deutschland und in der Ukraine in digitalen Medien verwendet wurden, zu analysieren, die strukturell-semantischen Merkmale ausgewählter phraseologischer Einheiten in beiden Sprachen zu beschreiben, ihre konzeptuelle Analyse durchzuführen, die vorhandenen Neologismenpaare einander gegenüberzustellen sowie Äquivalenztypen zu untersuchen. Die Materialbasis bilden diesbezüglich 96 deutsche und 60 ukrainische phraseologische Neologismen der Jahre 2020–2021.
NOSTRIN belongs to the recently defined F-BAR protein family. F-BAR proteins are
multi-domain proteins, which serve as adaptors between plasma membrane and
cytoskeleton components in processes such as membrane protrusion formation,
endocytosis and migration. NOSTRIN encompasses a F-BAR domain at the N-terminus,
which mediates membrane association, followed by a HR1 motif and an intermediate
domain (ID) domain in the middle, and a SH3 domain at the C-terminus. The domain
architecture and ability to form oligomers enable NOSTRIN to coordinate several
interaction partners namely dynamin, caveolin, N-WASP and endothelial nitric oxide
synthase (eNOS) in the process of eNOS trafficking. In this context NOSTRIN was
originally identified and hence termed eNOS traffick inducer. NOSTRIN is expressed in
vascularized tissues (e.g. liver and lung) and in primary endothelial cells.
Aims of the present work were (1) to investigate if NOSTRIN is involved in other
processes besides eNOS trafficking, (2) to analyse the function of NOSTRIN in vivo
through knockdown of NOSTRIN in developing zebrafish and (3) to study the
consequences of the loss of NOSTRIN on signal transduction in a primary cell culture
model derived from NOSTRIN knockout mice.
To study the possible involvement of NOSTRIN in other processes besides eNOS
trafficking a yeast two-hybrid screen was performed in which fibroblast growth factor
receptor 1 (FGFR1) was identified as a putative novel interaction partner of NOSTRIN. In
a series of yeast two-hybrid, pulldown and co-immunoprecipitation experiments the
interaction between NOSTRIN and FGFR1 was confirmed to occur between
endogenously expressed proteins and determined to be direct and to depend on the ID
domain of NOSTRIN and the 130 C-terminal amino acid residues of FGFR1. FGFR1 is
activated by binding of fibroblast growth factors (FGFs) and induces several different
signal transduction pathways (e.g. MAPK and Akt pathway). Overexpression of
NOSTRIN in HeLa cells specifically enhanced FGF2-dependent MAPK activation.
Accordingly, depletion of NOSTRIN attenuated FGF2-dependent MAPK activation and
did not affect FGF2-induced Akt activation.
In summary, NOSTRIN has been identified as a novel interaction partner of FGFR1
involved in FGF2-dependent signal transduction.
The morpholino oligonucleotide-mediated knockdown of NOSTRIN in developing
zebrafish caused vascular leakage and irregular vascular patterning e.g. a loss of the
proper trajectory of intersegmental vessel and interruptions of the dorsal longitudinal
anastomotic vessel. The vascular phenotype was consistent upon use of two different
morpholinos and could be rescued in a dose dependent manner by the injection of
zebrafish NOSTRIN mRNA. Detailed analysis involving confocal and time lapse
microscopy in zebrafish with endothelial specific expression of EGFP revealed that the
knockdown of NOSTRIN impacts in vivo on the migration and morphology of endothelial
tip cells and leads to a reduction of filopodia number and length.
Additionally a NOSTRIN knockout mouse was generated. The analysis of FGFR1 signal
transduction in primary mouse lung endothelial cells (MLECs) from NOSTRIN knockout
and wild type mice revealed that FGF2-dependent MAPK activation was attenuated in
MLECs isolated from NOSTRIN knockout mice when compared to MLECs isolated from
wild type mice. The effect of NOSTRIN on FGF2-dependent signal transduction seems to
be specific, since VEGF-induced MAPK activation was not affected in NOSTRIN
knockout MLECs. The importance of NOSTRIN for FGF2 signal transduction in vivo is
demonstrated by the greatly impaired angiogenic response to FGF2 in NOSTRIN
knockout mice in matrigel plug assay. In a detailed biochemical analysis it was
discovered that NOSTRIN interacts with the activated small GTPase Rac1 and that
overexpression of NOSTRIN enhances Rac1 activation. Furthermore, the interactions of
NOSTRIN with both Rac1 and its GEF Sos1 are required for NOSTRIN-mediated
activation of Rac1. In accordance, activation of Rac1 was not detected upon FGF2
stimulation in NOSTRIN knockout MLECs.
In conclusion, the present work describes a novel function of the F-BAR protein
NOSTRIN in FGFR1 signal transduction. Data presented in this work demonstrate that
NOSTRIN is required for the assembly of a complex consisting of FGFR1, Sos1 and
Rac1 and subsequently for the FGF2-dependent activation of Rac1 in endothelial cells.
U radu se preispituju granice frazema s obzirom na tvorenice kao frazemske sastavnice (krenulo je niz brdo (nizbrdo) što, ići na ruku (naruku) komu) ili samostalne jedinice (bogtepitaj, budibogsnama, kvragu) koje prema postojećoj frazemskoj definiciji ne ulaze u frazeološki fond. Posebna se pozornost poklanja frazemskim polusloženicama kao novoustanovljenoj skupini frazema prema frazemskome opsegu (amo-tamo, brže-bolje, danas-sutra, rak-rana, više-manje, zbrda-zdola). Istraživanje je utemeljeno na korpusu hrvatskih pravopisa od Partaša do Babića i Moguša, hrvatskim općim i frazeološkim rječnicima te elektroničkom korpusu Hrvatska jezična riznica Instituta za hrvatski jezik i jezikoslovlje.
Parni prijedlozi
(2007)
U ovome se članku analiziraju poredbeni frazemi jednoga hrvatskog čakavskog govora prikupljeni na terenskome dijalektološkom istraživanju i uspoređuju s poredbenim frazemima hrvatskoga standardnog jezika. Posebna se pozornost poklanja onim čakavskim frazemima koji imaju različitu sliku u pozadini frazema unutar istoga frazemskog koncepta i onima koji nemaju ekvivalenta u standardu.
U radu se račlanjuje odnos reda riječi i negacije u hrvatskome crkvenoslavenskome jeziku. Prvi se vid toga odnosa tiče položaja niječnih izraza ne, ni i bez. U zadanome korpusu oni stoje ispred, odnosno lijevo od jezične jedinice koju niječu. Jedinica ispred koje se niječni izraz nalazi nije nužno glagol, pa ni finitni glagolski oblik. Položaj niječnih izraza povezan je i s razlikom, odnosno s utvrđivanjem razlike između sastavničke negacije, kao negacije nepredikatne sastavnice, i rečenične negacije, negacije predikata. Konačno, o redu riječi ovisi hoće li se ili neće provesti niječno slaganje. U hrvatskome je crkvenoslavenskome jeziku provođenje niječnoga slaganja djelomično (ne i proizvoljno), a ovisi o tome na kojemu se mjestu u rečenici nalazi niječna zamjenica ili prilog.
This paper offers an overview of the presence of the german language and culture in Serbia. The focus lies on the region Vojvodina, as it has the greatest significance for the spread of the German language, culture and literature in Serbia, and there German as a foreign language is still strongly represented in schools. At the same time, it presents the status of the German teacher training in Serbia and discusses some perspectives in this domain.
Two main types of methods are used in gene therapy: integrating vectors and nuclease-based genome engineering. Nucleases are site-specific and are efficient for knock-outs, but inefficient at inserting long DNA sequences. Integrating vectors perform this task with high efficiency, but their insertion occurs at random genomic positions. This can result in transformation of target cells, which leads to severe adverse events in a gene therapy context. Thus, it is of great interest to develop novel genome engineering tools that combine the advantages of both technologies. The main focus of this thesis is on generating such a targetable integrating vector.
The integrating vector used in this project is the Sleeping Beauty (SB) transposon, a DNA transposon characterized by high activity across a wide range of cells. The SB transposase was combined with an RNA-guided Cas9 nuclease domain. This nuclease component was meant to direct transposase integration to specific targets defined by RNAs. The SB transposase was fused to cleavage-inactivated Cas9 (dCas9) to tether it to the target sites. In addition, adapter proteins consisting of dCas9 and domains non-covalently interacting with SB transposase or the SB transposon were generated. All constituent domains of these fusion proteins were tested in enzymatic assays and almost all enzymatic activities could be verified.
Combining the fusion protein dCas9-SB100X with a gRNA binding a sequence from the AluY repetitive element resulted in a weak, but statistically significant enrichment around sites bound by the gRNA. This enrichment was ca. 2-fold and occurred within a 300 bp window downstream of target sites, or within the AluY element.
Targeting with adapter proteins and targeting of other targets (L1 elements or single-copy targets) did not result in statistically significant effects. Single-copy targets tested included the HPRT gene and three specifically selected GSH targets that were known to be receptive to SB insertions. The combination with a more sequence-specific transposase mutant also failed to increase specificity to a level allowing targeting of single-copy loci. Genome-wide analysis of insertions however demonstrated, that dCas9-SB100X has a different insertion profile than SB100X, regardless of the gRNA used.
As low efficiency of retargeting is likely a consequence of the high background activity of the SB100X transposase in the fusion constructs, a SB mutant with reduced DNA affinity, SB(C42), was generated. For this mutant, transposition activity was partly dependent on a dCas9 domain being supplied with a multi-copy target gRNA, specifically a 2-fold increase in the presence of a AluY-directed gRNA. Whether using this mutant results in improved targeting remains to be determined.
In a side project, an attempt was made to direct SB insertions to ribosomal DNA by fusing the transposase to a nucleolar protein. This fusion transposase partially localized to nucleoli and insertions catalyzed by this transposase were found to be enriched in nucleolus organizer regions (NORs) and nucleolus-associated domains (NADs).
The aim of a second side project was increasing the ratio between homology-directed repair (HDR) and non-homologous end-joining (NHEJ) at Cas9-mediated double-strand breaks (DSBs). To achieve this, Cas9 was fused to DNA-interacting domains and corresponding binding sequences were fused to the homology donors. While an increased HDR/NHEJ ration could be observed for the fusion proteins, it was not dependent on the presence on the binding sequences in the donor molecules.
In an ideal world all investment products, including hedge funds, would be marketable to all investors. In this ideal world, all investors would fully understand the nature of the products and would be able to make an informed choice whether to invest. Of course the ideal world does not exist – the retail investment market is characterised by asymmetries of information. Product providers know most about the products on offer (or at least they should do). Investment advisers often know rather less than the provider but much more than their retail customers. Providers and intermediary advisers are understandably motivated by the desire to sell their products. There is therefore a risk that investment products will be mis-sold by investment advisers or mis-bought by ill-informed investors. This asymmetry of information is dealt with in most countries through regulation. However, the regulatory response in different countries is not necessarily the same. There are various ways in which protections can be applied and it is important to understand that the cultural background and regulatory histories of countries flavours the way regulation has developed. This means (as will be explained in greater detail later) that some countries are better able than others to admit hedge funds to the retail sector. Following this Introduction, Section II looks at some key background issues. Section III then looks at some important questions raised by the retail hedge fund issue. Many of these are questions of balance. Balance lies at the heart of regulation of course – regulation must always balance the needs of investors and with market efficiency. Understanding the “retail hedge fund” question requires particular attention to balance. Section IV then looks at the UK regime and how the FSA has answered the balance question. Section V offers some international perspectives. Section VI concludes. It will be seen that there is no obviously right answer to the question whether hedge fund products should be marketed to retail investors. Each regulator in each jurisdiction needs to make up its own mind on how to deal with the various issues and balances. It is evident, however, that internationally there is a move towards a greater variety of retail funds. There is nothing wrong with that, provided the regulators and the retail customers they protect, understand sufficiently what sort of protection is, or is not, being offered in the regulatory regime.
This Paper will look at the changing nature of asset management, and will examine the nature of the European framework for collective investment undertakings, enshrined in the UCITS Directive2 in that light. This question whether the UCITS Directive in its current form remains an appropriate European response to the changing investment management landscape is an issue with which the European Commission is actively engaging through its Green Paper on the Enhancement of the EU Framework for Investment Funds, published in July 2005.3 But before considering these important questions, it is necessary to begin with an idea of what a collective investment, more specifically a UCITS actually is and how it fits conceptually in the broader world of pooled investments.....
Aufgrund der starken Heterogenität und Komplexität der akuten myeloischen Leukämie ist diese bis heute nicht zufriedenstellend zu behandeln. Die bestmögliche Therapie wird mittlerweile zunehmend auf die Erkrankung des Einzelnen angepasst. Vermehrt gewinnen Tyrosinkinase-Inhibitoren in der Therapie an Bedeutung. Diese Inhibitoren hemmen Proteine auf zellulärer Ebene.
Bei etwa 30% der AML-Patienten lassen sich Mutationen des FLT3-Gens nachweisen. Das Gen kodiert für die fms like tyrosine kinase 3, eine Rezeptor-Tyrosinkinase an der Zelloberfläche von unreifen Blutzellen des Knochenmarks. Durch Mutationen des FLT3 Gens erhalten diese Zellen einen Proliferationsvorteil gegenüber den physiologischen Blutzellen.
Am häufigsten kommt es zu in frame-Insertionen des FLT3-Gens, vor allem im Bereich der juxtamembranen Domäne: sogenannte interne Tandemduplikationen (ITD). Weiterhin kommen zu einem geringeren Teil Punktmutationen einzelner Codons, zum Beispiel im Bereich des activation loops oder im Bereich des gatekeepers vor. Durch das Auftreten der Punktmutationen, die entweder bereits zum Zeitpunkt der Diagnose vorliegen oder erst während einer Therapie mit einem Tyrosinkinase-Inhibitor entstehen können, verändert sich das Bindungsverhalten vieler solcher gegen FLT3 gerichteten Inhibitoren. Durch Letzteres kann ein mögliches Therapieversagen beispielsweise während der Behandlung mit AC220 (Quizartinib) erklärt werden (Smith et al.).
In der vorliegenden Dissertationsschrift sind Unterschiede der Signalwege zwischen FLT3-ITD und FLT3-ITD mit der zusätzlichen gatekeeper-Punktmutation F691L herausgearbeitet. Dafür wurden die beiden FLT3-Mutationen in den Vektor pMy-IRES-GFP eingebracht und retroviral in Ba/F3-Zellen transduziert. Nach Überprüfung der Expression von FLT3 ITD und dem Wachstumsverhalten unter Zugabe von AC220 (Quizartinib), wurden verschiedene Signalkaskaden von FLT3 mittels Western Blot untersucht. Hierbei zeigten sich sowohl Unterschiede für die Expression von phosphoryliertem ERK als auch von phosphoryliertem STAT5.
Durch verschieden starke Expressionen der FLT3130kDa- und FLT3160kDA-Varianten wurde eine unterschiedliche Lokalisation von FLT3-ITD in Zellen mit und ohne die Mutation F691L postuliert. Allerdings ließ sich diese experimentell mittels Immunfluoreszenz nicht belegen, da die Methode für die verwendeten Suspensionszellen nicht ausreichend geeignet war.
In den durchgeführten Versuchen zum Wachstumsverhalten der Zellen bei der Verwendung von Kinaseinhibitoren konnte bei der Verwendung des SYK-Inhibitors R406 eine dosisabhängige Proliferationshemmung der FLT3-ITD-mutierten Ba/F3- und 32D-Zellen beobachtet werden. Die Hemmung von FLT3 durch R406 wurde in der Literatur bereits beschrieben (Braselmann et al.).
Die abschließenden Experimente der Massenspektrometrie mit SILAC Markierung lassen mit der Detektion von mehreren hundert signifikant regulierten phosphorylierten Proteinen in den beiden FLT3-ITD-exprimierenden Populationen auf die Aktivierung unterschiedlicher Signalwege schließen. Durch das Vergleichen einzelner Teilexperimente ergaben sich Proteine, deren Phosphorylierung mehrfach in die gleiche Richtung reguliert war. Für Zellen, die zusätzlich zur ITD-Mutation die Mutation F691L besaßen, konnten insgesamt sieben hoch-regulierte, phosphorylierte Proteine ermittelt werden, bei denen ein zellulärer Effekt durch die Phosphorylierung der entsprechenden Aminosäurereste in der Literatur beschrieben ist.
Das im Western Blot nachgewiesene, in Zellen mit der Mutation F691L stärker phosphorylierte STAT5 ist aller Voraussicht nach Ursache der nachgewiesenen verstärkten Phosphorylierung von RPS6 im Experiment der globalen Phosphorylierung. Die PIM-Kinasen als Substrate einer STAT5-induzierten Transkription phosphorylieren RPS6 an Serin 235. Dies führt seinerseits zu einer verstärkten Translation von mRNA weiterer Gene. Die genauen Zusammenhänge der hier ermittelten Unterschiede müssen jedoch weiter untersucht werden.
In Zukunft könnte zudem die Untersuchung der beiden Proteine SHP 1 oder HSP90 weitere Aufschlüsse über die unterschiedlichen Signalwege geben. Für beide Proteine wurden Phosphorylierungen detektiert, die in den untersuchten Zellen mit FLT3-ITD bzw. der zusätzlichen Punktmutation F691L unterschiedlich reguliert sind.
Rhodopsins are the most universal biological light-energy transducers and abundant phototrophic mechanisms that evolved on Earth and have a remarkable diversity and potential for biotechnological applications. Recently, the first sodium-pumping rhodopsin KR2 from Krokinobacter eikastus was discovered and characterized. However, the existing structures of KR2 are contradictory, and the mechanism of Na+ pumping is not yet understood. Here, we present a structure of the cationic (non H+) light-driven pump at physiological pH in its pentameric form. We also present 13 atomic structures and functional data on the KR2 and its mutants, including potassium pumps, which show that oligomerization of the microbial rhodopsin is obligatory for its biological function. The studies reveal the structure of KR2 at nonphysiological low pH where it acts as a proton pump. The structure provides new insights into the mechanisms of microbial rhodopsins and opens the way to a rational design of novel cation pumps for optogenetics.
One of the aims of the SPARC collaboration [1] at FAIR is to perform precision atomic physics expe- riments with highly charged heavy ions at the High Energy Storage Ring (HESR). An internal target is indis- pensably an integral part for many such experiments. Ions with different charge states, which are obtained as a result of interaction of an ion beam with the target, need to be effectively separated and detected. In this work we present ion optical studies unambiguously showing the feasibility of SPARC experiments at the HESR.
Se discute aquí la reseña de Friedrich Blanckenburg sobre "Las penas del joven Werther" (1774) de J. W. Goethe. La reseña, de la que ofrecemos la traducción al español de varios pasajes, fue publicada unos meses más tarde que la polémica y revolucionaria novela de Goethe y constituye un documento valioso no solo de su recepción, sino también de la teorización acerca del género en el contexto de finales de la Segunda Ilustración en Alemania. Da cuenta, en efecto, de un rasgo central de este periodo: la recepción sensibilista, 'empfindsam', que tendría un efecto benefactor sobre la sociabilidad y las costumbres. Además, en una relación en cierto modo ambigua con este llamado a la empatía socialmente útil del crítico y del lector, el autor presenta elementos que señalan llamativamente hacia una doctrina de la autonomía de la esfera estética. Lo que queda sin saberse es si se trata de una "desprolijidad" de la reseña o de una lúcida interpretación de la "voz doble" que plantea Goethe en su famosa novela epistolar.
"Österreich - USA: Künstlerischer und interkultureller Dialog". Konferenz der Austrian Studies Association (ASA) an der Universität Wien, 14.-17. März 2016
Zum zweiten Mal seit Bestehen der 1961 ins Leben gerufenen Austrian Studies Association (ASA) fand die jährliche ASA-Konferenz in Europa, und zwar wieder in Wien, statt. Organisiert wurde sie von Pia Janke (Forschungsplattform Elfriede Jelinek, Universität Wien), Maria-Regina Kecht (Rice University) und Teresa Kovacs (Forschungsplattform Elfriede Jelinek, Universität Wien). Die Veranstaltung vernetzte durch ein vielfältiges Programm an wissenschaftlichen Vorträgen und künstlerischen Programmpunkten die Universität Wien mit anderen in Wien beheimateten Institutionen, die als Kooperationspartner fungierten, namentlich mit dem Wien Museum, dem Österreichischen Filmmuseum und dem Amerika-Haus.
"… die ehernen Blöcke männlichen Schaffens umkreisen" - Elfriede Jelinek queert Lessing und Goethe
(2016)
Der Beitrag verschränkt kommunikations-, informations-, kulturwissenschaftliche sowie philosophische Ansätze zur Störung mit gender- und queer theory, um Elfriede Jelineks 'Gattung' des Sekundärdramas analytisch zu beschreiben. Jelinek verfasst ihre Sekundärdramen zu kanonisierten Dramen des deutschsprachigen Raums und stellt über ihr typisches, intertextuelles Verfahren Bezug zu den Stücken her, fordert gleichzeitig aber auch die Kombination der Sekundärdramen mit ihren Bezugstexten im Moment der Inszenierung und geht damit über ihr bisheriges Verfahren hinaus. Ausgehend von der Feststellung, dass Jelineks Sekundärdramen in den Umsetzungen am Theater meist als weibliche Gegenschreibung interpretiert werden, will der vorliegende Beitrag zeigen, dass die Sekundärdramen vielmehr an einer Auflösung der Kategorien von 'Weiblichkeit' und 'Männlichkeit' arbeiten. Ein besonderes Augenmerk liegt dabei auf der Thematisierung des Inzests, der mit Judith Butler als vorhandene Ordnungen und Relationen verschiebendes Element gelesen werden kann.
Endothelial to mesenchymal transition in cardiovascular disease : JACC state-of-the-art review
(2019)
Endothelial to mesenchymal transition (EndMT) is a process whereby an endothelial cell undergoes a series of molecular events that lead to a change in phenotype toward a mesenchymal cell (e.g., myofibroblast, smooth muscle cell). EndMT plays a fundamental role during development, and mounting evidence indicates that EndMT is involved in adult cardiovascular diseases (CVDs), including atherosclerosis, pulmonary hypertension, valvular disease, and fibroelastosis. Therefore, the targeting of EndMT may hold therapeutic promise for treating CVD. However, the field faces a number of challenges, including the lack of a precise functional and molecular definition, a lack of understanding of the causative pathological role of EndMT in CVDs (versus being a “bystander-phenomenon”), and a lack of robust human data corroborating the extent and causality of EndMT in adult CVDs. Here, we review this emerging but exciting field, and propose a framework for its systematic advancement at the molecular and translational levels.
Substantia nigra dopamine (SN DA) neurons are progressively lost in Parkinson disease (PD). While the molecular and cellular mechanisms of their differential vulnerability and degeneration have been extensively studied, we still know very little about potential functional adaptations of those SN DA neurons that – at least for some time – manage to survive during earlier stages of PD. We utilized a partial lesion 6-OHDA mouse model to characterize initial electrophysiological impairments and chronic adaptations of surviving identified SN DA neurons, both in vivo and in vitro. Early after lesion (3 weeks), we detected a selective loss of in vivo burst firing in surviving SN DA neurons, which was accompanied by in vitro pacemaker instability. In contrast, late after lesion (>2 months), in vivo firing properties of surviving SN DA neurons had recovered in the presence of 2-fold accelerated pacemaking in vitro. Finally, we show that this chronic cell-autonomous adaptation in surviving SN DA neurons was mediated by Kv4.3 channel downregulation. Our study demonstrates substantial homeostatic plasticity of surviving SN DA neurons after a single-hit non-progressive lesion, which might contribute to the phenotype of initially surviving SN DA neurons in PD.
Osteosarcomas are aggressive bone tumours with a high degree of genetic heterogeneity, which has historically complicated driver gene discovery. Here we sequence exomes of 31 tumours and decipher their evolutionary landscape by inferring clonality of the individual mutation events. Exome findings are interpreted in the context of mutation and SNP array data from a replication set of 92 tumours. We identify 14 genes as the main drivers, of which some were formerly unknown in the context of osteosarcoma. None of the drivers is clearly responsible for the majority of tumours and even TP53 mutations are frequently mapped into subclones. However, >80% of osteosarcomas exhibit a specific combination of single-base substitutions, LOH, or large-scale genome instability signatures characteristic of BRCA1/2-deficient tumours. Our findings imply that multiple oncogenic pathways drive chromosomal instability during osteosarcoma evolution and result in the acquisition of BRCA-like traits, which could be therapeutically exploited.
Multiple exponence in morphology has recently attracted a good deal of attention (see, among others, Harris 2017; Caballero & Inkelas 2018). In this paper, I examine Modern Greek verbs which take an extra verbalizer (implicit multiple exponence). The simple base (bare form) and the base with the verbalizer co-exist in the lexicon without any semantic or aspectual opposition and can be used in the same syntactic context. Thus, they raise important questions for morphological theory. I argue that the explanation of this pleonastic addition may be hidden in the relation between inflection and derivation and the polyfunctional character of verbalizers in synthetic languages. Since the two forms co-exist and one member of each pair features an idiomatic association of meaning and complex form, morphological theory is challenged. I argue that these formations find a natural account within the framework of Construction Morphology (Booij 2010; Jackendoff & Audring 2019).
Known by many names, the Chinese banyan (Ficus microcarpa L.) is a monoecious plant species originating from south-eastern Asia which has been introduced as an ornamental in numerous areas outside its native range, including the Mediterranean. Like every species of Ficus, it is associated with a series of chalcid wasp species, known as fig wasps. These species are distributed in the families Agaonidae, Epichrysomallidae, Eurytomidae, Ormyridae and Pteromalidae. In this publication, we describe a new species of Ormyrus Westwood, 1832 (Hymenoptera, Chalcidoidea, Ormyridae), O. microcarpae Askew & Koutsoukos sp. nov., reared from figs of F. microcarpa collected from Greece and Cyprus. The new species is compared with O. lini and O. watshami. This species is likely to be a parasitoid of Meselatus bicolor Chen, 1999 (Hymenoptera, Epichrysomallidae). In addition, the previously unknown female of O. lini is also described and illustrated. This publication constitutes the first report of species of Ormyrus associated with figs in Europe.
To unravel the short-term climate variability during Marine Isotope Stage (MIS) 11, which represents a close analogue to the Holocene with regard to orbital boundary conditions, we performed microfacies and time series analyses on a ~3200-yr-long record of annually laminated Holsteinian lake sediments from Dethlingen, northern Germany. These biogenic varves comprise two sub-layers: A light layer, which is controlled by spring/summer diatom blooms, and a dark layer consisting mainly of amorphous organic matter and fragmented diatom frustules deposited during autumn/winter. Time series analyses were performed on the thickness of the light and dark layers. Signals exceeding the 95 % and 99 % confidence levels occur at periods that are near-identical to those known from modern instrumental data and Holocene palaeoclimatic records. Spectral peaks at periods of 90, 25, and 10.5 yr are likely associated with the 88-, 22- and 11-yr solar cycles, respectively. This variability is mainly expressed in the light layer spectra, suggesting solar influence on the palaeoproductivity of the lake. Significant signals at periods between 3 and 5 yr and at ~6 yr are strongest expressed in the dark layer spectra and may reflect an influence of the El Niño-Southern Oscillation (ENSO) and the North Atlantic Oscillation (NAO) during autumn/winter. Our results suggest that solar forcing and ENSO/NAO-like variability influenced central European climate during MIS 11 similar to the present interglacial, thus demonstrating the comparability of the two interglacial periods at sub-decadal to decadal timescales.
To unravel the short-term climate variability during Marine Isotope Stage (MIS) 11, which represents a close analogue to the Holocene with regard to orbital boundary conditions, we performed microfacies and time series analyses on a ~3200-yr-long record of annually laminated Holsteinian lake sediments from Dethlingen, northern Germany. These biogenic varves comprise two sub-layers: a light sub-layer, which is controlled by spring/summer diatom blooms, and a dark sub-layer consisting mainly of amorphous organic matter and fragmented diatom frustules deposited during autumn/winter. Time series analyses were performed on the thickness of the light and dark sub-layers. Signals exceeding the 95% and 99% confidence levels occur at periods that are near-identical to those known from modern instrumental data and Holocene palaeoclimatic records. Spectral peaks at periods of 90, 25, and 10.5 yr are likely associated with the 88-, 22- and 11-yr solar cycles, respectively. This variability is mainly expressed in the light sub-layer spectra, suggesting solar influence on the palaeoproductivity of the lake. Significant signals at periods between 3 and 5 yr and at ∼6 yr are strongest expressed in the dark sub-layer spectra and may reflect an influence of the El Niño-Southern Oscillation (ENSO) and the North Atlantic Oscillation (NAO) during autumn/winter. Our results suggest that solar forcing and ENSO/NAO-like variability influenced central European climate during MIS 11 similarly to the present interglacial, thus demonstrating the comparability of the two interglacial periods at sub-decadal to decadal timescales.
Owing to long-term similarities with regard to orbital climate forcing (i.e., low eccentricity and a dampened influence of precession), Marine Isotope Stage (MIS) 11 represents one of the closest astronomical analogues for present and future climate. Hence, insights into the climate variability of MIS 11 can contribute to a better understanding of the climatic evolution of the present (Holocene) interglacial as it would occur without human interference. In order to elucidate the natural climate variability during MIS 11, this study examines predominantly annually laminated lake sediments of Holsteinian age from Dethlingen, northern Germany. The Holsteinian interglacial is widely accepted to be the terrestrial equivalent of MIS 11c in central Europe and can be biostratigraphically correlated with the Hoxnian, Mazovian and Praclaux interglacials on the British Isles, in Poland and in France, respectively. These correlations yield the potential to cross-check the results from individual sites on a regional scale. This study is based on a multi-proxy approach including palynological, micropaleontological, sedimentological, geochemical and time series analyses within a wellconstrained chronological framework that has been established through varve counting and regional bio-stratigraphic correlations with other annually laminated archives of Holsteinian age. In particular, the here-presented study aims at (i) fingerprinting the long-term (centennial- to millennial-scale) and short-term (sub-decadal- to decadal-scale) climate variability during the Holsteinian interglacial, (ii) deciphering the nature, tempo and trigger mechanisms of abrupt climate change under interglacial boundary conditions, and (iii) assessing its impact on terrestrial ecosystems. With regard to long-term climate variability, the vegetation succession at Dethlingen as inferred from pollen data provides insights into the mesocratic to telocratic forest phases of a glacial-interglacial cycle spanning ~11500 (± 1000) years of the 15-16-ka-long Holsteinian interglacial. The development of temperate mixed forests suggests a general prevalence of mild climatic conditions during the Holsteinian. The older parts of the interglacial are characterised by the strong presence of boreal tree taxa (e.g., Picea), whereas the younger parts of the interglacial are marked by the expansion of sub-Atlantic to Atlantic forest elements (e.g., Abies, Buxus, Ilex, Quercus) and the decline of boreal tree taxa. This vegetation succession suggests a general warming trend and decreasing seasonality over the course of the Holsteinian interglacial. Based on the maximum pollen abundances of indicator tree taxa (e.g., Buxus and Quercus), peak warmth was reached during the later stages of the interglacial; it was accompanied by high humidity. The forest succession of the Holsteinian interglacial was punctuated by abrupt and gradual changes in the abundances of temperate plant taxa. These vegetation changes indicate considerable intra-interglacial climate variability. In particular, two marked declines of temperate taxa leading to the transient development of boreal and sub-boreal forests were triggered by centennial-scale climate oscillations, here termed Older and Younger Holsteinian Oscillations (OHO and YHO). These oscillations occurred ~6000 and ~9000 years after the onset of the interglacial pioneer forestation in central Europe, respectively. To assess the impact of abrupt climate change on terrestrial ecosystems during the Holsteinian and to investigate the underlying driving mechanisms, the intervals spanning the OHO and the YHO at Dethlingen were subjected to decadal-scale palynological and sedimentological analyses. Based on these data, the OHO comprises a 90-year-long decline of temperate taxa associated with expansion of Pinus and non-arboreal pollen, and a subsequent 130-year-long recovery of temperate taxa marked by the pioneer expansion of Betula and Alnus. Owing to its highly characteristic imprint on vegetation dynamics, the OHO can be identified in pollen records from the central European lowlands north of 50º latitude, from the British Isles to Poland. A close inspection of individual pollen records from that region reveals the prevalence of colder winters during the OHO, with a gradient of decreasing temperature and moisture availability, and increased continentality towards eastern Europe. This climate pattern points to a weakened influence of the westerlies and/or stronger influence of the Siberian High connected to the OHO. The vegetation dynamics during the YHO are characterised by a decline of temperate taxa (particularly of Carpinus) and the expansion of pioneer trees (mainly Betula). In contrast to the OHO, frost-sensitive taxa (e.g., Ilex, Buxus and Hedera) continued to thrive. This suggests that mean winter temperatures remained relatively high (>0 ºC) during the YHO pointing to a decrease of summer warmth related to the climatic deterioration. The YHO, which has a duration on the order of 300 years, is centered within a long-term (~1500-year) decline and subsequent, millennial-scale recovery of temperate taxa. Because the impact of the OHO and the YHO on the vegetation at Dethlingen was markedly different, both short-term climate oscillations may have been caused by different trigger mechanisms. For the OHO, the inferred regional-scale winter cooling over central Europe lasting for several decades points to a decrease in ocean heat transport, most likely related to a transient slowdown in North Atlantic Deep Water formation. This view is supported by the strong resemblance of the OHO to the 8.2 ka event of the Holocene with regard to the duration, imprint on terrestrial ecosystems, spatial pattern of the climatic impact, timing within the respective interglacial, and prevailing interglacial boundary conditions. In contrast, the presence of frost-sensitive taxa during the YHO appears to exclude a reduction in oceanic heat transport as postulated for the OHO. Instead, the long-lasting, gradual changes in the abundances of temperate taxa suggest a connection to orbital forcing, with the triggering mechanism causing the centennial-scale vegetation setback itself remaining unclear. The characteristics of short-term climate variability were investigated based on microfacies and time series analyses of a ~3200-year-long, annually laminated window of the Dethlingen record. The annual laminations at Dethlingen comprise biogenic varves consisting of two discrete sub-layers. The light layers, which are controlled by the intensity of diatoms blooms during spring/summer, reflect changes in the productivity of the Dethlingen palaeolake. In contrast, the dark layers, which consist predominantly of amorphous organic matter and fragmented diatom frustules, represent sediment deposition during autumn/winter. Spectral analyses of the thicknesses of the light and dark layers have revealed several peaks exceeding the 95% and 99% confidence levels that are near-identical to those known from modern instrumental data and Holocene records. Decadal-scale signals at periods of 90, 25, and 10.5 years are likely associated with the 88-, 22- and 11-year solar cycles; hence, solar activity appears to have been a forcing agent in productivity changes of the Dethlingen palaeolake. Sub-decadal-scale signals at periods between 3 and 5 years and ~6 years may reflect an influence of the El Niño-Southern Oscillation (ENSO) and the North Atlantic Oscillation (NAO) on varve formation during winter.
The Mediterranean realm, comprising the Mediterranean and Macaronesian regions, has long been recognized as one of the world’s biodiversity hotspots, owing to its remarkable species richness and endemism. Several hypotheses on biotic and abiotic drivers of species diversification in the region have been often proposed but rarely tested in an explicit phylogenetic framework. Here, we investigate the impact of both species-intrinsic and -extrinsic factors on diversification in the species-rich, cosmopolitan Limonium, an angiosperm genus with center of diversity in the Mediterranean. First, we infer and time-calibrate the largest Limonium phylogeny to date. We then estimate ancestral ranges and diversification dynamics at both global and regional scales. At the global scale, we test whether the identified shifts in diversification rates are linked to specific geological and/or climatic events in the Mediterranean area and/or asexual reproduction (apomixis). Our results support a late Paleogene origin in the proto-Mediterranean area for Limonium, followed by extensive in situ diversification in the Mediterranean region during the late Miocene, Pliocene, and Pleistocene. We found significant increases of diversification rates in the “Mediterranean lineage” associated with the Messinian Salinity Crisis, onset of Mediterranean climate, Plio-Pleistocene sea-level fluctuations, and apomixis. Additionally, the Euro-Mediterranean area acted as the major source of species dispersals to the surrounding areas. At the regional scale, we infer the biogeographic origins of insular endemics in the oceanic archipelagos of Macaronesia, and test whether woodiness in the Canarian Nobiles clade is a derived trait linked to insular life and a biotic driver of diversification. We find that Limonium species diversity on the Canary Islands and Cape Verde archipelagos is the product of multiple colonization events followed by in situ diversification, and that woodiness of the Canarian endemics is indeed a derived trait but is not associated with a significant shift to higher diversification rates. Our study expands knowledge on how the interaction between abiotic and biotic drivers shape the uneven distribution of species diversity across taxonomic and geographical scales.
This is the tenth article in our series Trouble on the Far-Right.
How can a racist party that was getting less than 0.2% of the vote for years, enter parliament with 18 MPs? How can a party that promotes violence, hate, sexism and murders amplify its reach after each pogrom? How can Golden Dawn remain the third political power in Greece for four years? And what’s in the mind of a Golden Dawner?...
Bi Tirga
(2011)
Leonard Bi Tirga, son of a poor peasant, is a studious pupil. Due to shortage of finances, he has to leave school to make ends meet and pursue his studies. Leonard becomes a sweatshop labourer. As a young labourer, his life like that of his peers is hard. The pay rate is low and the work is hard. With his friends, they engage in trade union activism. A series of complicated and trying events reinforces their conviction to militate. Thus, Leonard and his friend Camille become Union leaders. Leonard's character trait and uprightness explains the book title, Bi Tirga. In the Moore language, this means a well educated, honest, hardworking, courageous and well-behaved youth.
DEDICACES GRATITUDES INTRODUCTION- - - -- - - - - - - - - - - - - - - - - - - -7 Formulation, délimitation et choix du sujet- - -- - - - - - - - - - - - - - - - - - - - - 9 Hypothèse de recherche- - - - - - -- - - - - - - - - - - - - - - - - - - - - -11 Cadre méthodologique- - - - - - -- - - - - - - - - - - - - - - - - - - - - - - - 13 PREMIERE PARTIE : INSCRIPTION DU DON DANS L’OEUVRE D’AMINATA SOW FALL CHAPITRE I : TYPOLOGIE DES DONS- - - - - - - - - - - - - - - - - - - - - - - 17 I.1.Le don des biens et services I.2.Le don de soi et de temps CHAPITRE II : ACTEURS DU DON- - - - - - - - - - - - - - - - - - - - - - - - - - - - 19 II.1.Le donateur II.2.Le donataire II.3.L’intermédiaire CHAPITRE III : AUTOUR DU DON- - - - - - - - - - - - - - - - - - - - - - - - - - - -21 III.1.Le don comme forme d’échange III.2.Le don comme facteur d’intérêt DEUXIEME PARTIE : DON ET SOCIETE CHAPITRE IV : MOTIVATIONS DU DON- - - - - - - - - - - - - - - - - - - - - - -25 IV.1.La religion et l’indigence IV.2.La culture africaine CHAPITRE V : AVANTAGES DU DON- - - - - - - - - - - - - - - - - - - - - - - - - 27 V.1.Le don comme acte humanitaire V.2.Le don comme vecteur du lien social CHAPITRE VI : DESAGREMENTS DU DON- - - - - - - - - - - - - - - - - - - - -29 VI.1.L’émergence des classes VI.2.La dette morale CONCLUSION- - - - - - - - - - - - - - - - - - - - - - - - - - - - - 31 BIBLIOGRAPHIE- - - - - - - - - - - - - - - - - - - - - - - - - - - - 32 -------------------------------------------------------------------------------------------------- CRELAF (Cercle de Reflexion des Etudiants en Littératures Africaines), Département de Littératures Africaines, Université Omar Bongo, Gabon
« Et si les crocodiles pleuraient pour de vrai… » est une nouvelle dans laquelle, semblet-il, l’écrivain et théoricien Ludovic Emane Obiang paraît asseoir une conception du monde et de la littérature basée sur une figure profonde de même qu’un phénomène essentiel que sont la figure du crocodile et le phénomène de la reconstruction. Sa nouvelle paraît nous plonger au coeur d’une question essentielle source de véritables angoisses. Les crocodiles pleurent-ils ? Et s’ils pleurent, pleurent-ils pour de vrai ? A quoi devons nous faire attention lorsqu’on nous invite à penser à partir de la figure du crocodile ? Ensuite, en quoi cette figue de crocodile a-t-elle rapport avec quelque chose de l’ordre de la reconstruction, car comme le souligne Ludovic Obiang, le maître mot de sa nouvelle est le phénomène de la reconstruction ? Qu’est-ce que la reconstruction ? A quoi renvoie la réalité du crocodile ?
En 1999, un roman vient révolutionner la pratique littéraire au Gabon : Le jeune officier. OEuvre dédiée à Martin Heidegger, elle développe à sa manière les préoccupations du philosophe concernant l’être, l’oubli de l’être et le caractère aléthéique de la vérité. Les pages qui suivent sont consacrées à ce roman d’un style particulier qui insère le roman gabonais entre modernité et post-modernité. A travers un résumé, un commentaire du discours du jeune officier et la lecture du discours de soutenance de Brice levy Koumba1, nous tenterons de découvrir l’oeuvre. Le résumé est à la première personne, fait par le jeune officier lui-même. -------------------------------------------------------------------------------------------- CRELAF (Cercle de Reflexion des Etudiants en Littératures Africaines), Département de Littératures Africaines, Université Omar Bongo, Gabon
Un moment de ma vie
(2008)
Pour Eric Joël Békalé, la chasse illustre « le parcours de l’homme sur terre ». Ce parcours vise la réalisation de l’homme véritable. L’homme véritable, se conçoit comme « un être pas comme les autres1 ». A la base, il prend conscience que l’homme est un état qui se construit. Au final il n’a plus rien de commun car il devient « le début et la fin de toute chose». La chasse comme processus de réalisation de l’humain dans sa propriété, est aussi l’opération par laquelle l’homme quête son bonheur par le plaisir, le savoir et le pouvoir. Il doit pour se faire réduire la peur et trouver sa place. Car celui qui a peur n’est pas un homme et celui qui n’est pas un homme se prive l’accès au bonheur. « La chasse est une affaire d'homme. Que celui qui a peur de mourir mange les feuilles de manioc ».
L’analyse qui suit a pour objet la notion du double chez Alain Mabanckou. Cette notion est étudiée telle qu’elle se donne à lire dans Mémoire de porc-épic, roman paru en 2006 aux éditions du Seuil. Ce qui nous intéresse ici c’est le rapport possible existant entre double, parole et ponctuation. Présenté ainsi, nous cherchons à comprendre cette particularité du texte de Mabanckou qui est une longue phrase sans ponctuation, sinon n’étant ponctuée que par des virgules.
This paper proposes a new approach for modeling investor fear after rare disasters. The key element is to take into account that investors’ information about fundamentals driving rare downward jumps in the dividend process is not perfect. Bayesian learning implies that beliefs about the likelihood of rare disasters drop to a much more pessimistic level once a disaster has occurred. Such a shift in beliefs can trigger massive declines in price-dividend ratios. Pessimistic beliefs persist for some time. Thus, belief dynamics are a source of apparent excess volatility relative to a rational expectations benchmark. Due to the low frequency of disasters, even an infinitely-lived investor will remain uncertain about the exact probability. Our analysis is conducted in continuous time and offers closed-form solutions for asset prices. We distinguish between rational and adaptive Bayesian learning. Rational learners account for the possibility of future changes in beliefs in determining their demand for risky assets, while adaptive learners take beliefs as given. Thus, risky assets tend to be lower-valued and price-dividend ratios vary less under adaptive versus rational learning for identical priors. Keywords: beliefs, Bayesian learning, controlled diffusions and jump processes, learning about jumps, adaptive learning, rational learning. JEL classification: D83, G11, C11, D91, E21, D81, C61
Modern macroeconomics empirically addresses economy-wide incentives behind economic actions by using insights from the way a single representative household would behave. This analytical approach requires that incentives of the poor and the rich are strictly aligned. In empirical analysis a challenging complication is that consumer and income data are typically available at the household level, and individuals living in multimember households have the potential to share goods within the household. The analytical approach of modern macroeconomics would require that intra-household sharing is also strictly aligned across the rich and the poor. Here we have designed a survey method that allows the testing of this stringent property of intra-household sharing and find that it holds: once expenditures for basic needs are subtracted from disposable household income, household-size economies implied by the remainder household incomes are the same for the rich and the poor.
Most simulated micro-founded macro models use solely consumer-demand aggregates in order to estimate deep economy-wide preference parameters, which are useful for policy evaluation. The underlying demand-aggregation properties that this approach requires, should be easy to empirically disprove: since household-consumption choices differ for households with more members, aggregation can be rejected if appropriate data violate an affine equation regarding how much individuals benefit from within-household sharing of goods. We develop a survey method that tests the validity of this equation, without utility-estimation restrictions via models. Surprisingly, in six countries, this equation is not rejected, lending support to using consumer-demand aggregates.