Refine
Year of publication
Document Type
- Article (31128)
- Part of Periodical (11550)
- Book (8266)
- Doctoral Thesis (5713)
- Part of a Book (3967)
- Working Paper (3385)
- Review (2939)
- Contribution to a Periodical (2368)
- Preprint (2084)
- Report (1560)
Language
- German (42718)
- English (29217)
- French (1060)
- Portuguese (840)
- Spanish (309)
- Croatian (302)
- Multiple languages (258)
- Italian (198)
- mis (174)
- Turkish (168)
Has Fulltext
- yes (75568) (remove)
Keywords
- Deutsch (1076)
- Literatur (868)
- taxonomy (760)
- Deutschland (553)
- Rezension (511)
- new species (449)
- Rezeption (354)
- Frankfurt <Main> / Universität (341)
- Übersetzung (326)
- Geschichte (300)
Institute
- Medizin (7691)
- Präsidium (5170)
- Physik (4464)
- Extern (2738)
- Wirtschaftswissenschaften (2686)
- Gesellschaftswissenschaften (2369)
- Biowissenschaften (2184)
- Biochemie und Chemie (1975)
- Frankfurt Institute for Advanced Studies (FIAS) (1686)
- Center for Financial Studies (CFS) (1628)
Background: Infection is a main cause of morbidity and mortality after heart surgery, with multi-resistant pathogens increasingly representing a challenge. Daptomycin provides bactericidal activity against gram-positive organisms that are resistant to standard treatment including vancomycin.
Methods: A cohort of cardiac surgical patients, treated with daptomycin for major infection at two tertiary care centers, were retrospectively studied with a particular focus on the type of infection, causative pathogens and co-infections, daptomycin dosage, adverse events and outcome in order to provide evidence for the efficiency and safety of daptomycin in a distinct high-risk patient population.
Results: Sixty-five patients (87.7 % males, 60.4 ± 13.5 years) who had undergone aortic surgery (20.0 %), ventricular assist device (VAD) implantation (21.5 %), combined procedures (21.5 %), coronary artery bypass grafting (12.3 %), isolated valve surgery (15.4 %) and heart transplantation (7.7 %) were diagnosed with catheter-related infection (26.1 %), valve endocarditis (18.8 %), sternal wound (13.0 %), VAD-associated (11.6 %), cardiac implantable electrophysiological device (CIED)-associated (4.1 %), respiratory tract (4.3 %), bloodstream (4.3 %) and other infection (4.3 %). In 13.0 %, no focus of infection was identified though symptoms of severe infection were present. The most frequent pathogens were Staphylococcus epidermidis (30.4 %), Staphylococcus aureus (23.1 %) and Enterococcus species (10.1 %). Daptomycin doses ranging from 3 mg/kg every 48 h to 10 mg/kg every 24 h were administered for 15.4 ± 11.8 days. 87.0 % of the cases were classified as success, 7.2 % as treatment failure and 5.8 as non-evaluable. Adverse events were limited to one case of mild and one case of moderate neutropenia with recovery upon termination of treatment.
Conclusion: Daptomycin proved safe and effective in major infection in high-risk cardiac surgical patients.
The family of scaffold attachment factor B (SAFB) proteins comprises three members and was first identified as binders of the nuclear matrix/scaffold. Over the past two decades, SAFBs were shown to act in DNA repair, mRNA/(l)ncRNA processing, and as part of protein complexes with chromatin-modifying enzymes. SAFB proteins are approximately-100-kDa-sized dual nucleic acid-binding proteins with dedicated domains in an otherwise largely unstructured context, but whether and how they discriminate DNA- and RNA-binding has remained enigmatic. We here provide the SAFB2 DNA- and RNA-binding SAP and RRM domains in their functional boundaries and use solution NMR spectroscopy to ascribe DNA- and RNA-binding functions. We give insight into their target nucleic acid preferences and map the interfaces with respective nucleic acids on sparse data-derived SAP and RRM domain structures. Further, we provide evidence that the SAP domain exhibits intra-domain dynamics and a potential tendency to dimerise, which may expand its specifically targeted DNA sequence range. Our data provide a first molecular basis of and a starting point towards deciphering DNA- and RNA-binding functions of SAFB2 on the molecular level and serve a basis for understanding its localization to specific regions of chromatin and its involvement in the processing of specific RNA species.
The family of scaffold attachment factor B (SAFB) proteins comprises three members and was first identified as binders of the nuclear matrix/scaffold. Over the past two decades, SAFBs were shown to act in DNA repair, mRNA/(l)ncRNA processing and as part of protein complexes with chromatin-modifying enzymes. SAFB proteins are approximately 100 kDa-sized dual nucleic acid-binding proteins with dedicated domains in an otherwise largely unstructured context, but whether and how they discriminate DNA and RNA binding has remained enigmatic. We here provide the SAFB2 DNA- and RNA-binding SAP and RRM domains in their functional boundaries and use solution NMR spectroscopy to ascribe DNA- and RNA-binding functions. We give insight into their target nucleic acid preferences and map the interfaces with respective nucleic acids on sparse data-derived SAP and RRM domain structures. Further, we provide evidence that the SAP domain exhibits intra-domain dynamics and a potential tendency to dimerize, which may expand its specifically targeted DNA sequence range. Our data provide a first molecular basis of and a starting point towards deciphering DNA- and RNA-binding functions of SAFB2 on the molecular level and serve a basis for understanding its localization to specific regions of chromatin and its involvement in the processing of specific RNA species.
The current outbreak of the highly infectious COVID-19 respiratory disease is caused by the novel coronavirus SARS-CoV-2 (Severe Acute Respiratory Syndrome Coronavirus 2). To fight the pandemic, the search for promising viral drug targets has become a cross-border common goal of the international biomedical research community. Within the international Covid19-NMR consortium, scientists support drug development against SARS-CoV-2 by providing publicly available NMR data on viral proteins and RNAs. The coronavirus nucleocapsid protein (N protein) is an RNA-binding protein involved in viral transcription and replication. Its primary function is the packaging of the viral RNA genome. The highly conserved architecture of the coronavirus N protein consists of an N-terminal RNA-binding domain (NTD), followed by an intrinsically disordered Serine/Arginine (SR)-rich linker and a C-terminal dimerization domain (CTD). Besides its involvement in oligomerization, the CTD of the N protein (N-CTD) is also able to bind to nucleic acids by itself, independent of the NTD. Here, we report the near-complete NMR backbone chemical shift assignments of the SARS-CoV-2 N-CTD to provide the basis for downstream applications, in particular site-resolved drug binding studies.
The ongoing pandemic caused by the Betacoronavirus SARS-CoV-2 (Severe Acute Respiratory Syndrome Coronavirus-2) demonstrates the urgent need of coordinated and rapid research towards inhibitors of the COVID-19 lung disease. The covid19-nmr consortium seeks to support drug development by providing publicly accessible NMR data on the viral RNA elements and proteins. The SARS-CoV-2 genome encodes for approximately 30 proteins, among them are the 16 so-called non-structural proteins (Nsps) of the replication/transcription complex. The 217-kDa large Nsp3 spans one polypeptide chain, but comprises multiple independent, yet functionally related domains including the viral papain-like protease. The Nsp3e sub-moiety contains a putative nucleic acid-binding domain (NAB) with so far unknown function and consensus target sequences, which are conceived to be both viral and host RNAs and DNAs, as well as protein-protein interactions. Its NMR-suitable size renders it an attractive object to study, both for understanding the SARS-CoV-2 architecture and drugability besides the classical virus’ proteases. We here report the near-complete NMR backbone chemical shifts of the putative Nsp3e NAB that reveal the secondary structure and compactness of the domain, and provide a basis for NMR-based investigations towards understanding and interfering with RNA- and small-molecule-binding by Nsp3e.
Wie wir unseren Tod verloren : Biopolitik, Raum und Unheimlichkeit zwischen Neuzeit und Moderne
(2011)
Matthias Korn befasst sich mit einem Phänomen, das für gewöhnlich unter dem Signum der 'Verdrängung des Todes' gefasst wird. Er argumentiert jedoch, dass in Anbetracht der historischen Dokumente über den Umgang mit dem Tod und den Toten seit dem Mittelalter präziser von einem Ausschleichprozess gesprochen werden muss. Am Beispiel des neuzeitlichen Verhältnisses zum eigenen Tod als einem Gegenüber (Descartes) zeichnet Korn die biopolitischen Maßnahmen nach, die zu seiner allmählichen gesellschaftlichen Marginalisierung geführt haben und in die Errichtung von Friedhöfen außerhalb der Städte mündeten. Dies wird anschließend mit der Methode des Physiologen Xavier Bichat verglichen, wobei der Nachweis erbracht wird, dass die 'medizinisch-experimentelle' Dezentralisierung des Todes als analoger Versuch zu verstehen ist, dessen Unheimlichkeit zu bannen.
The early Tournaisian (Early Carboniferous; Mississippian) ammonoids from the classical abandoned limestone quarry of Gattendorf (Upper Franconia) are revised, using the historical collections as well as so far undescribed material. The ammonoid assemblage is composed of prionoceratid ammonoids of the six genera Mimimitoceras, Paragattendorfia, Stockumites, Acutimitoceras, Gattendorfia and Gattenpleura, which indicate a stratigraphic position near the Devonian–Carboniferous boundary in the earliest Carboniferous. The new species Stockumites hofensis sp. nov. and S. nonaginta sp. nov. are described.
The railway cutting near Oberrödinghausen at the northern margin of the Rhenish Mountains is the cardinal section for the investigation of Early Tournaisian (Early Carboniferous; Mississippian) ammonoids. The ammonoids from the Hangenberg Limestone (= Gattendorfia Limestone) of this and neighbouring outcrops are revised here, using the historical collections as well as undescribed new material. The ammonoid assemblages are composed of a total of 67 species, which occur in four successive ammonoid zones. The assemblages are composed of predominant prionoceratids (Order Goniatitina) with the twenty genera Mimimitoceras (two species), Globimitoceras (one species), Paragattendorfia (two species), Kornia (three species), Stockumites (eleven species), Acutimitoceras (two species), Costimitoceras (one species), Nicimitoceras (four species), Imitoceras (one species), Voehringerites (one species), Gattendorfia (eight species), Zadelsdorfia (two species), Kazakhstania (one species), Gattenpleura (one species), Weyerella (three species), Hasselbachia (three species), Paprothites (five species), Pseudarietites (three species), Rodingites (two species), Paralytoceras (one species) as well as subordinate eocanitids (Order Prolecanitida) with the genera Eocanites (eight species) and Nomismocanites (one species). The new genera Rodingites gen. nov. and Nomismocanites gen. nov. as well as the new species Mimimitoceras perditum sp. nov., Kornia fibula sp. nov., Kornia acia sp. nov., Stockumites parallelus sp. nov., Stockumites voehringeri sp. nov., Acutimitoceras ucatum sp. nov., Acutimitoceras paracutum sp. nov., Imitoceras initium sp. nov., Gattendorfia rhenana sp. nov., Gattendorfia bella sp. nov., Gattendorfia valdevoluta sp. nov., Gattendorfia schmidti sp. nov., Gattendorfia corpulenta sp. nov., Gattendorfia immodica sp. nov., Zadelsdorfia oblita sp. nov., Weyerella lenis sp. nov., Hasselbachia erronea sp. nov., Paprothites beckeri sp. nov., Paprothites kullmanni sp. nov., Eocanites delicatus sp. nov. and Nomismocanites raritas gen. et sp. nov. are described from Oberrödinghausen. Mimimitoceras mina sp. nov., Stockumites marocensis sp. nov., Zadelsdorfia zana sp. nov. and Kazakhstania kana sp. nov. are newly named for material from the Anti-Atlas of Morocco.
Early Carboniferous coiled nautiloids from North Africa are virtually unknown. An assemblage of nine species, all from the family Trigonoceratidae, from the Dalle à Merocanites (Tournaisian-Viséan boundary interval) of Timimoun in western Algeria is described, being the most diverse Carboniferous nautiloid assemblage known from North Africa but much less diverse than the time-equivalent assemblages from Belgium and Ireland. The assemblage consists of the species Maccoyoceras pentagonum sp. nov., Lispoceras orbis sp. nov., Thrincoceras devolvere sp. nov., Rineceras multituberculatum sp. nov., Rineceras rectangulatum sp. nov., Vestinautilus padus sp. nov., Vestinautilus concinnus sp. nov., Planetoceras destrictum sp. nov. and Planetoceras transforme sp. nov. A morphometric analysis of Maccoyoceras pentagonum sp. nov. and Lispoceras orbis sp. nov. shows that the intraspecific variation in these species is within rather narrow limits.
Viséan coiled nautiloids from North Africa are only poorly known. From the Mougoui Ayoun, Zrigat and Hamou-Rhanem formations of the eastern Anti-Atlas, we describe coiled nautiloids, which belong to the genera Rineceras, Stroboceras, Temnocheilus, Vestinautilus, Maccoyoceras, Endolobus, Epidomatoceras, Liroceras, Ephippioceras, and Solenochilus. The new species Temnocheilus imazighenorum sp. nov., Temnocheilus aubrechtovae sp. nov., Vestinautilus kesslerae sp. nov., Endolobus rota sp. nov., Epidomatoceras ebbighausenorum sp. nov., Liroceras vermis sp. nov., Liroceras karaouii sp. nov., Ephippioceras pygops sp. nov., Solenochilus lucynae sp. nov. and Solenochilus pohlei sp. nov. are described; six taxa are kept in open nomenclature. The assemblage is composed of the three superfamilies Trigonoceratoidea, Clydonautiloidea and Aipoceratoidea and shows a wide spectrum of conch morphologies, ranging from widely umbilicate compressed forms to involute compact forms, reflecting a broad ecological variation.