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A novel osteotomy preparation technique to preserve implant site viability and enhance osteogenesis
(2019)
Chen, Chih-Hao ; Coyac, Benjamin R. ; Arioka, Masaki ; Leahy, Brian ; Tulu, U. Serdar ; Aghvami, Maziar ; Holst, Stefan ; Hoffmann, Waldemar ; Quarry, Antony ; Bahat, Oded ; Salmon, Benjamin ; Brunski, John ; Helms, Jill A.
The preservation of bone viability at an osteotomy site is a critical variable for subsequent implant osseointegration. Recent biomechanical studies evaluating the consequences of site preparation led us to rethink the design of bone-cutting drills, especially those intended for implant site preparation. We present here a novel drill design that is designed to efficiently cut bone at a very low rotational velocity, obviating the need for irrigation as a coolant. The low-speed cutting produces little heat and, consequently, osteocyte viability is maintained. The lack of irrigation, coupled with the unique design of the cutting flutes, channels into the osteotomy autologous bone chips and osseous coagulum that have inherent osteogenic potential. Collectively, these features result in robust, new bone formation at rates significantly faster than those observed with conventional drilling protocols. These preclinical data have practical implications for the clinical preparation of osteotomies and alveolar bone reconstructive surgeries.
Saintas, Emily ; Abrahams, Liam ; Ahmad, Gulshan T. ; Ajakaiye, Anu-Oluwa M. ; AlHumaid, Abdulaziz S. H. A. M. ; Ashmore-Harris, Candice ; Clark, Iain ; Dura, Usha K. ; Fixmer, Carine N. ; Ike-Morris, Chinedu ; Prado, Mireia Mato ; Mccullough, Danielle ; Mishra, Shishir ; Schöler, Katia M. U. ; Timur, Husne ; Williamson, Maxwell D. C. ; Alatsatianos, Markella ; Bahsoun, Basma ; Blackburn, Edith ; Hogwood, Catherine E. ; Lithgow, Pamela E. ; Rowe, Michelle ; Yiangou, Lyto ; Rothweiler, Florian ; Cinatl, Jindrich ; Zehner, Richard ; Baines, Anthony J. ; Garrett, Michelle ; Gourlay, Campbell W. ; Griffin, Darren K. ; Gullick, William J. ; Hargreaves, Emma ; Howard, Mark ; Lloyd, Daniel ; Rossman, Jeremy S. ; Smales, C. Mark ; Tsaousis, Anastasios D. ; Haar, Tobias von der ; Wass, Mark N. ; Michaelis, Martin
The formation of acquired drug resistance is a major reason for the failure of anti-cancer therapies after initial response. Here, we introduce a novel model of acquired oxaliplatin resistance, a sub-line of the non-MYCN-amplified neuroblastoma cell line SK-N-AS that was adapted to growth in the presence of 4000 ng/mL oxaliplatin (SK-N-ASrOXALI4000). SK-N-ASrOXALI4000 cells displayed enhanced chromosomal aberrations compared to SK-N-AS, as indicated by 24-chromosome fluorescence in situ hybridisation. Moreover, SK-N-ASrOXALI4000 cells were resistant not only to oxaliplatin but also to the two other commonly used anti-cancer platinum agents cisplatin and carboplatin. SK-N-ASrOXALI4000 cells exhibited a stable resistance phenotype that was not affected by culturing the cells for 10 weeks in the absence of oxaliplatin. Interestingly, SK-N-ASrOXALI4000 cells showed no cross resistance to gemcitabine and increased sensitivity to doxorubicin and UVC radiation, alternative treatments that like platinum drugs target DNA integrity. Notably, UVC-induced DNA damage is thought to be predominantly repaired by nucleotide excision repair and nucleotide excision repair has been described as the main oxaliplatin-induced DNA damage repair system. SK-N-ASrOXALI4000 cells were also more sensitive to lysis by influenza A virus, a candidate for oncolytic therapy, than SK-N-AS cells. In conclusion, we introduce a novel oxaliplatin resistance model. The oxaliplatin resistance mechanisms in SK-N-ASrOXALI4000 cells appear to be complex and not to directly depend on enhanced DNA repair capacity. Models of oxaliplatin resistance are of particular relevance since research on platinum drugs has so far predominantly focused on cisplatin and carboplatin.
Rajan, Roopa ; Divya, K. P. ; Kandadai, Rukmini Mridula ; Yadav, Ravi ; Satagopam, Venkata P. ; Satagopam, Venkata P. ; Madhusoodanan, U. K. ; Agarwal, Pankaj ; Kumar, Niraj ; Ferreira, Teresa ; Kumar, Hrishikesh ; Prasad, A. V. Sreeram ; Shetty, Kuldeep ; Mehta, Sahil ; Desai, Soaham ; Kumar, Suresh ; Prashant, L. K. ; Bhatt, Mohit ; Wadia, Pettarusp ; Ramalingam, Sudha ; Wali, G. M. ; Pandey, Sanjay ; Bartusch, Felix ; Hannussek, Maximilian ; Krüger, Jens ; Kumar-Sreelatha, Ashwin ; Grover, Sandeep ; Lichtner, Peter ; Sturm, Marc ; Roeper, Jochen ; Busskamp, Volker ; Chandak, Giriraj R. ; Schwamborn, Jens ; Seth, Pankaj ; Gasser, Thomas ; Rieß, Olaf ; Goyal, Vinay ; Goyal, Vinay ; Pal, Pramod Kumar ; Borgohain, Rupam ; Krüger, Rejko ; Kishore, Asha ; Sharma, Manu
Over the past two decades, our understanding of Parkinson's disease (PD) has been gleaned from the discoveries made in familial and/or sporadic forms of PD in the Caucasian population. The transferability and the clinical utility of genetic discoveries to other ethnically diverse populations are unknown. The Indian population has been under-represented in PD research. The Genetic Architecture of PD in India (GAP-India) project aims to develop one of the largest clinical/genomic bio-bank for PD in India. Specifically, GAP-India project aims to: (1) develop a pan-Indian deeply phenotyped clinical repository of Indian PD patients; (2) perform whole-genome sequencing in 500 PD samples to catalog Indian genetic variability and to develop an Indian PD map for the scientific community; (3) perform a genome-wide association study to identify novel loci for PD and (4) develop a user-friendly web-portal to disseminate results for the scientific community. Our “hub-spoke” model follows an integrative approach to develop a pan-Indian outreach to develop a comprehensive cohort for PD research in India. The alignment of standard operating procedures for recruiting patients and collecting biospecimens with international standards ensures harmonization of data/bio-specimen collection at the beginning and also ensures stringent quality control parameters for sample processing. Data sharing and protection policies follow the guidelines established by local and national authorities.We are currently in the recruitment phase targeting recruitment of 10,200 PD patients and 10,200 healthy volunteers by the end of 2020. GAP-India project after its completion will fill a critical gap that exists in PD research and will contribute a comprehensive genetic catalog of the Indian PD population to identify novel targets for PD.
Gorelik, Tatiana ; Habermehl, Stefan ; Shubin, Aleksandr A. ; Gruene, Tim ; Yoshida, Kaname ; Oleynikov, Peter ; Kaiser, Ute ; Schmidt, Martin U.
Copper perchlorophthalocyanine (CuPcCl16, CuC32N8Cl16, Pigment Green 7) is one of the commercially most important green pigments. The compound is a nanocrystalline fully insoluble powder. Its crystal structure was first addressed by electron diffraction in 1972 [Uyeda et al. (1972). J. Appl. Phys. 43, 5181–5189]. Despite the commercial importance of the compound, the crystal structure remained undetermined until now. Using a special vacuum sublimation technique, micron-sized crystals could be obtained. Three-dimensional electron diffraction (3D ED) data were collected in two ways: (i) in static geometry using a combined stage-tilt/beam-tilt collection scheme and (ii) in continuous rotation mode. Both types of data allowed the crystal structure to be solved by direct methods. The structure was refined kinematically with anisotropic displacement parameters for all atoms. Due to the pronounced crystal mosaicity, a dynamic refinement was not feasible. The unit-cell parameters were verified by Rietveld refinement from powder X-ray diffraction data. The crystal structure was validated by many-body dispersion density functional theory (DFT) calculations. CuPcCl16 crystallizes in the space group C2/m (Z = 2), with the molecules arranged in layers. The structure agrees with that proposed in 1972.
Calaminus, Gabriele ; Schneider, Dominik T. ; Schweinitz, Dietrich von ; Jürgens, Heribert ; Infed, Nacera ; Schönberger, Stefan ; Olson, Thomas A. ; Albers, Peter ; Vokuhl, Christian ; Stein, Raimund ; Looijenga, Leendert ; Sehouli, Jalid ; Metzelder, Martin ; Claviez, Alexander ; Dworzak, Michael ; Eggert, Angelika ; Fröhlich, Birgit ; Gerber, Nicolas U. ; Kratz, Christian P. ; Faber, Jörg ; Klingebiel, Thomas ; Harms, Dieter ; Göbel, Ulrich
Objective: To evaluate prognostic factors in pediatric patients with gonadal germ cell tumors (GCT). Methods: Patients <18 years with ovarian and testicular GCT (respectively OGCT and TGCT) were prospectively registered according to the guidelines of MAKEI 96. After resection of the primary tumor, patients staged ≥II received risk-stratified cisplatin-based combination chemotherapy. Patients were analyzed in respect to age (six age groups divided into 3-year intervals), histology, stage, and therapy. The primary end point was overall survival. Results: Between January 1996 and March 2016, the following patients were registered: 1047 OGCT, of those, 630 had ovarian teratoma (OTER) and 417 had malignant OGCT (MOGCT); and 418 TGCT, of those, 106 had testicular teratoma (TTER) and 312 had malignant TGCT (MTGCT). Only in MTGCT, older age correlated with a higher proportion of advanced tumors. All 736 teratomas and 240/415 stage I malignant gonadal GCT underwent surgery and close observation alone. In case of watchful waiting, the progression rate of OGCT was higher than that of TGCT. However, death from disease was reported in 8/417 (1.9%) MOGCT and 8/312 (2.6%) MTGCT irrespective of adjuvant chemotherapy and repeated surgery. Conclusions: The different pathogenesis and histogenesis of gonadal GCT reflects sex- and age-specific patterns that define clinically relevant risk groups. Therefore, gender and age should be considered in further research on the biology and clinical practice of pediatric gonadal GCT.
Kocsis, Vilmos ; Kaib, David A. S. ; Riedl, Kira Marina ; Gass, Sebastian ; Lampen-Kelley, Paula ; Mandrus, David G. ; Nagler, Stephen E. ; Pérez Rodriguez, Nicolás ; Nielsch, Kornelius ; Büchner, Bernd ; Wolter, Anja U. B. ; Valentí, Roser
The Kitaev material α-RuCl3 is among the most prominent candidates to host a quantum spin-liquid state endowed with fractionalized excitations. Recent experimental and theoretical investigations have separately revealed the importance of both the magnetoelastic coupling and the magnetic anisotropy, in dependence of the applied magnetic field direction. In this combined theoretical and experimental research, we investigate the anisotropic magnetic and magnetoelastic properties for magnetic fields applied along the main crystallographic axes as well as for fields canted out of the honeycomb plane. We found that the magnetostriction anisotropy is unusually large compared to the anisotropy of the magnetization, which is related to the strong magnetoelastic Γ′˜-type coupling in our \textit{ab-initio} derived model. We observed large, non-symmetric magnetic anisotropy for magnetic fields canted out of the honeycomb ab-plane in opposite directions, namely towards the +c∗ or −c∗ axes, respectively. The observed directional anisotropy is explained by considering the relative orientation of the magnetic field with respect to the co-aligned RuCl6 octahedra. Magnetostriction measurements in canted fields support this non-symmetric magnetic anisotropy, however these experiments are affected by magnetic torque effects. Comparison of theoretical predictions with experimental findings allow us to recognize the significant contribution of torque effects in experimental setups where α-RuCl3 is placed in canted magnetic fields.
Podoliak, Elizaveta ; Lamm, Gerrit H. U. ; Alekseev, Alexey ; Marin, Egor ; Schellbach, Anna V. ; Fedotov, Daniil A. ; Stetsenko, Artem ; Maliar, Nina ; Bourenkov, Gleb ; Balandin, Taras ; Baeken, Christian ; Astashkin, Roman ; Schneider, Thomas R. ; Bateman, Alex ; Wachtveitl, Josef ; Schapiro, Igor ; Busskamp, Volker ; Guskov, Albert ; Gordeliy, Valentin ; Kovalev, Kirill
Light-driven sodium pumps (NaRs) are unique ion-transporting microbial rhodopsins. The major group of NaRs is characterized by an NDQ motif and has two aspartic acid residues in the central region essential for sodium transport. Here we identified a new subgroup of the NDQ rhodopsins bearing an additional glutamic acid residue in the close vicinity to the retinal Schiff base. We thoroughly characterized a member of this subgroup, namely the protein ErNaR from Erythrobacter sp. HL-111 and showed that the additional glutamic acid results in almost complete loss of pH sensitivity for sodium-pumping activity, which is in contrast to previously studied NaRs. ErNaR is capable of transporting sodium efficiently even at acidic pH levels. X-ray crystallography and single particle cryo-electron microscopy reveal that the additional glutamic acid residue mediates the connection between the other two Schiff base counterions and strongly interacts with the aspartic acid of the characteristic NDQ motif. Hence, it reduces its pKa. Our findings shed light on a new subgroup of NaRs and might serve as a basis for their rational optimization for optogenetics.
CD4+ T cell lymphopenia predicts mortality from Pneumocystis pneumonia in kidney transplant patients
(2020)
Freiwald, Tilo ; Büttner, Stefan ; Cheru, Nardos Tesfaye ; Avaniadi, Despina ; Martin, Simon S. ; Stephan, Christoph ; Pliquett, Rainer U. ; Asbe-Vollkopf, Aida ; Schüttfort, Gundolf ; Jacobi, Volkmar ; Herrmann, Eva ; Geiger, Helmut ; Hauser, Ingeborg A.
Background: Pneumocystis jirovecii pneumonia (PcP) remains a life-threatening opportunistic infection after solid organ transplantation, even in the era of Pneumocystis prophylaxis. The association between risk of developing PcP and low CD4+ T cell counts has been well established. However, it is unknown whether lymphopenia in the context of post-renal transplant PcP increases the risk of mortality. Methods: We carried out a retrospective analysis of a cohort of kidney transplant patients with PcP (n = 49) to determine the risk factors for mortality associated with PcP. We correlated clinical and demographic data with the outcome of the disease. For CD4+ T cell counts, we used the Wilcoxon rank sum test for in-hospital mortality and a Cox proportional-hazards regression model for 60-day mortality. Results: In univariate analyses, high CRP, high neutrophils, CD4+ T cell lymphopenia, mechanical ventilation, and high acute kidney injury network stage were associated with in-hospital mortality following presentation with PcP. In a receiver-operator characteristic (ROC) analysis, an optimum cutoff of ≤200 CD4+ T cells/µL predicted in-hospital mortality, CD4+ T cell lymphopenia remained a risk factor in a Cox regression model. Conclusions: Low CD4+ T cell count in kidney transplant recipients is a biomarker for disease severity and a risk factor for in-hospital mortality following presentation with PcP.
Pliquett, Rainer U. ; Asbe-Vollkopf, Aida ; Scheuermann, Ernst-Heinrich ; Gröne, Elisabeth ; Probst, Michael ; Geiger, Helmut ; Hauser, Ingeborg A.
Introduction Impaired renal function and/or pre-existing atherosclerosis in the deceased donor increase the risk of delayed graft function and impaired long-term renal function in kidney transplant recipients. Case presentation We report delayed graft function occurring simultaneously in two kidney transplant recipients, aged 57-years-old and 39-years-old, who received renal allografts from the same deceased donor. The 62-year-old donor died of cardiac arrest during an asthmatic state. Renal-allograft biopsies performed in both kidney recipients because of delayed graft function revealed cholesterol-crystal embolism. An empiric statin therapy in addition to low-dose acetylsalicylic acid was initiated. After 10 and 6 hemodialysis sessions every 48 hours, respectively, both renal allografts started to function. Glomerular filtration rates at discharge were 26 ml/min/1.73 m2 and 23.9 ml/min/1.73 m2, and remained stable in follow-up examinations. Possible donor and surgical procedure-dependent causes for cholesterol-crystal embolism are discussed. Conclusion Cholesterol-crystal embolism should be considered as a cause for delayed graft function and long-term impaired renal allograft function, especially in the older donor population.
Adler, Clemens ; Ahammed, Zubayer ; Allgower, C. ; Amonett, J. ; Anderson, B.D. ; Anderson, M. ; Averichev, Georgy S. ; Balewski, J. ; Barannikova, O. ; Barnby, L.S. ; Baudot, J. ; Bekele, S. ; Belaga, V.V. ; Bellwied, Rene ; Berger, J. ; Bichsel, H. ; Billmeier, A. ; Bland, L.C. ; Blyth, C.O. ; Bonner, B.E. ; Boucham, A. ; Brandin, A. ; Bravar, A. ; Cadman, R.V. ; Caines, Helen Louise ; Calderón de la Barca Sánchez, Manuel ; Cardenas, A. ; Carroll, J. ; Castillo Castellanos, Javier Ernesto ; Castro, M. ; Cebra, D. ; Chaloupka, Petr ; Chattopadhyay, S. ; Chen, Y. ; Chernenko, S.P. ; Cherney, M. ; Chikanian, A. ; Choi, B. ; Christie, William ; Coffin, J.P. ; Cormier, T.M. ; Cramer, J.G. ; Crawford, H.J. ; Deng, W.S. ; Derevschikov, A.A. ; Didenko, L. ; Dietel, Thomas ; Draper, J.E. ; Dunin, V.B. ; Dunlop, J.C. ; Eckardt, V. ; Efimov, L.G. ; Emelianov, V. ; Engelage, J. ; Eppley, G. ; Erazmus, B. ; Fachini, P. ; Faine, V. ; Filimonov, K. ; Finch, E. ; Fisyak, Y. ; Flierl, D. ; Foley, K.J. ; Fu, J. ; Gagliardi, Carl A. ; Gagunashvili, N. ; Gans, J. ; Gaudichet, L. ; Germain, M. ; Geurts, F. ; Ghazikhanian, V. ; Grachov, O. ; Grigoriev, Vladislav A. ; Guedon, M. ; Gushin, E. ; Hallman, T.J. ; Hardtke, D. ; Harris, J.W. ; Henry, T.W. ; Heppelmann, S. ; Herston, T. ; Hippolyte, Boris ; Hirsch, A. ; Hjort, E. ; Hoffmann, Gerald W. ; Horsley, M. ; Huang, H.Z. ; Humanic, Thomas J. ; Igo, G. ; Ishihara, A. ; Ivanshin, Yu.I. ; Jacobs, P. ; Jacobs, W.W. ; Janik, M. ; Johnson, I. ; Jones, Peter Graham ; Judd, Eleanor G. ; Kaneta, M. ; Kaplan, M. ; Keane, Declan ; Kiryluk, J. ; Kisiel, A. ; Klay, J. ; Klein, S.R. ; Klyachko, A. ; Konstantinov, A.S. ; Kopytine, M. ; Kotchenda, L. ; Kovalenko, A.D. ; Kramer, M. ; Kravtsov, P. ; Krueger, Keith William ; Kuhn, C. ; Kulikov, A.I. ; Kunde, G.J. ; Kunz, C.L. ; Kutuev, R.Kh. ; Kuznetsov, A.A. ; Lakehal-Ayat, L. ; Lamont, Matthew A. C. ; Landgraf, J.M. ; Lange, S. ; Lansdell, C.P. ; Lasiuk, B. ; Laue, F. ; Lebedev, A. ; Lednický, R. ; Leontiev, V.M. ; LeVine, M.J. ; Li, Q. ; Lindenbaum, S.J. ; Lisa, Michael A. ; Liu, F. ; Liu, L. ; Liu, Z. ; Liu, Q.J. ; Ljubicic, T. ; Llope, W.J. ; LoCurto, G. ; Long, H. ; Longacre, R.S. ; Lopez-Noriega, M. ; Love, W.A. ; Ludlam, T. ; Lynn, D. ; Ma, J. ; Majka, R. ; Margetis, Spyridon ; Markert, Christina ; Martin, L. ; Marx, J. ; Matis, Howard S. ; Matulenko, Yu.A. ; McShane, T.S. ; Meissner, F. ; Melnick, Yu. ; Meschanin, A. ; Messer, M. ; Miller, M.L. ; Milosevich, Z. ; Minaev, N.G. ; Mitchell, J. ; Moiseenko, V.A. ; Moore, C.F. ; Morozov, V. ; de Moura, M.M. ; Munhoz, M.G. ; Nelson, J.M. ; Nevski, P. ; Nikitin, V.A. ; Nogach, L.V. ; Norman, B. ; Nurushev, S.B. ; Odyniec, G. ; Ogawa, A. ; Okorokov, Vitaly A. ; Oldenburg, M. ; Olson, D. ; Paic, Guy ; Pandey, S.U. ; Panebratsev, Y. ; Panitkin, S.Y. ; Pavlinov, A.I. ; Pawlak, T. ; Perevoztchikov, V. ; Peryt, W. ; Petrov, V.A. ; Planinic, M. ; Pluta, J. ; Porile, N. ; Porter, J. ; Poskanzer, A.M. ; Potrebenikova, E. ; Prindle, D. ; Pruneau, C. ; Putschke, Jörn H. ; Rai, G. ; Rakness, G. ; Ravel, O. ; Ray, R.L. ; Razin, S.V. ; Reichhold, D. ; Reid, Jeffrey G. ; Retiere, F. ; Ridiger, A. ; Ritter, H.G. ; Roberts, J.B. ; Rogachevski, O.V. ; Romero, J.L. ; Rose, A. ; Roy, C. ; Rykov, V. ; Sakrejda, I. ; Salur, Sevil ; Sandweiss, J. ; Saulys, A.C. ; Savin, I. ; Schambach, Joachim ; Scharenberg, R.P. ; Schmitz, N. ; Schroeder, L.S. ; Schüttauf, A. ; Schweda, Kai ; Seger, J. ; Seliverstov, D. ; Seyboth, P. ; Shahaliev, E. ; Shestermanov, K.E. ; Shimanskii, S.S. ; Shvetcov, V.S. ; Skoro, G. ; Smirnov, Nikolai ; Snellings, Raimond ; Sorensen, P. ; Sowinski, J. ; Spinka, Harold. M. ; Srivastava, B. ; Stephenson, E.J. ; Stock, Reinhard ; Stolpovsky, A. ; Strikhanov, M. ; Stringfellow, B. ; Struck, C. ; Suaide, Alexandre Alarcon do Passo ; Sugarbaker, E. ; Suire, Christophe Pierre ; Šumbera, Michal ; Surrow, Bernd ; Symons, T.J.M. ; Szanto de Toledo, A. ; Szarwas, P. ; Tai, A. ; Takahashi, Jun ; Tang, A.H. ; Thomas, James H. ; Thompson, M. ; Tikhomirov, V. ; Tokarev, M. ; Tonjes, M.B. ; Trainor, T.A. ; Trentalange, Stephen ; Tribble, Robert E. ; Trofimov, V. ; Tsai, Oleg D. ; Ullrich, Thomas ; Underwood, D.G. ; Van Buren, G. ; VanderMolen, A.M. ; Vasilevski, I.M. ; Vasiliev, A.N. ; Vigdor, S.E. ; Voloshin, S.A. ; Wang, Fuqiang ; Ward, H. ; Watson, J.W. ; Wells, R. ; Westfall, Gary D. ; Whitten, C. ; Wieman, H. ; Willson, R. ; Wissink, S.W. ; Witt, Richard ; Wood, J. ; Xu, N. ; Xu, Z. ; Yakutin, A.E. ; Yamamoto, E. ; Yang, J. ; Yepes, P. ; Yurevich, V.I. ; Zanevski, Y.V. ; Zborovský, I. ; Zhang, H. ; Zhang, W.M. ; Zoulkarneev, R. ; Zubarev, A.N.
Mid-rapidity transverse mass spectra and multiplicity densities of charged and neutral kaons are reported for Au + Au collisions at √sNN = 130 GeV at RHIC. The spectra are exponential in transverse mass, with an inverse slope of about 280 MeV in central collisions. The multiplicity densities for these particles scale with the negative hadron pseudo-rapidity density. The charged kaon to pion ratios are K+/π− = 0.161± 0.002(stat) ± 0.024(syst) and K−/π− = 0.146± 0.002(stat) ± 0.022(syst) for the most central collisions. The K+/π− ratio is lower than the same ratio observed at the SPS while the K−/π− is higher than the SPS result. The ratios are enhanced by about 50% relative to p + p and p¯ + p collision data at similar energies.