Refine
Document Type
- Preprint (238)
- Article (128)
- Working Paper (1)
Has Fulltext
- yes (367)
Is part of the Bibliography
- no (367)
Keywords
Institute
- Physik (363)
- Frankfurt Institute for Advanced Studies (FIAS) (330)
- Informatik (314)
- Medizin (2)
- Biowissenschaften (1)
Multiplicity dependence of inclusive J/ψ production at midrapidity in pp collisions at √s = 13 TeV
(2020)
Measurements of the inclusive J/ψ yield as a function of charged-particle pseudorapidity density dNch/dη in pp collisions at √s = 13 TeV with ALICE at the LHC are reported. The J/ψ meson yield is measured at midrapidity (|y| < 0.9) in the dielectron channel, for events selected based on the charged-particle multiplicity at midrapidity (|η| < 1) and at forward rapidity (−3.7 < η < −1.7 and 2.8 < η < 5.1); both observables are normalized to their corresponding averages in minimum bias events. The increase of the normalized J/ψ yield with normalized dNch/dη is significantly stronger than linear and dependent on the transverse momentum. The data are compared to theoretical predictions, which describe the observed trends well, albeit not always quantitatively.
Systematic studies of charge-dependent two- and three-particle correlations in Pb-Pb collisions at sNN−−−√ = 2.76 and 5.02 TeV used to probe the Chiral Magnetic Effect (CME) are presented. These measurements are performed for charged particles in the pseudorapidity (η) and transverse momentum (pT) ranges |η| < 0.8 and 0.2 < pT < 5 GeV/c. A significant charge-dependent signal that becomes more pronounced for peripheral collisions is reported for the CME-sensitive correlators γ1, 1 = 〈cos(φα + φβ − 2Ψ2)〉 and γ1, − 3 = 〈cos(φα − 3φβ + 2Ψ2)〉. The results are used to estimate the contribution of background effects, associated with local charge conservation coupled to anisotropic flow modulations, to measurements of the CME. A blast-wave parametrisation that incorporates local charge conservation tuned to reproduce the centrality dependent background effects is not able to fully describe the measured γ1,1. Finally, the charge and centrality dependence of mixed-harmonics three-particle correlations, of the form γ1, 2 = 〈cos(φα + 2φβ − 3Ψ3)〉, which are insensitive to the CME signal, verify again that background contributions dominate the measurement of γ1,1.
The production of π±, K±, K0S, K∗(892)0, p, ϕ(1020), Λ, Ξ−, Ω−, and their antiparticles was measured in inelastic proton–proton (pp) collisions at a center-of-mass energy of s√ = 13 TeV at midrapidity (|y|<0.5) as a function of transverse momentum (pT) using the ALICE detector at the CERN LHC. Furthermore, the single-particle pT distributions of K0S, Λ, and Λ¯¯¯¯ in inelastic pp collisions at s√=7 TeV are reported here for the first time. The pT distributions are studied at midrapidity within the transverse momentum range 0≤pT≤20 GeV/c, depending on the particle species. The pT spectra, integrated yields, and particle yield ratios are discussed as a function of collision energy and compared with measurements at lower s√ and with results from various general-purpose QCD-inspired Monte Carlo models. A hardening of the spectra at high pT with increasing collision energy is observed, which is similar for all particle species under study. The transverse mass and xT≡2pT/s√ scaling properties of hadron production are also studied. As the collision energy increases from s√ = 7–13 TeV, the yields of non- and single-strange hadrons normalized to the pion yields remain approximately constant as a function of s√, while ratios for multi-strange hadrons indicate enhancements. The pT-differential cross sections of π±, K± and p (p¯¯¯) are compared with next-to-leading order perturbative QCD calculations, which are found to overestimate the cross sections for π± and p (p¯¯¯) at high pT.
J/ψ production as a function of charged-particle multiplicity in p–Pb collisions at √sNN = 8.16 TeV
(2020)
Inclusive J/ψ yields and average transverse momenta in p-Pb collisions at a center-of-mass energy per nucleon pair sNN−−−√ = 8.16 TeV are measured as a function of the charged-particle pseudorapidity density with ALICE. The J/ψ mesons are reconstructed at forward (2.03<ycms<3.53) and backward (−4.46<ycms<−2.96) center-of-mass rapidity in their dimuon decay channel while the charged-particle pseudorapidity density is measured around midrapidity. The J/ψ yields at forward and backward rapidity normalized to their respective average values increase with the normalized charged-particle pseudorapidity density, the former showing a weaker increase than the latter. The normalized average transverse momenta at forward and backward rapidity manifest a steady increase from low to high charged-particle pseudorapidity density with a saturation beyond the average value.
Rationale: RBPs (RNA binding proteins) play critical roles in the cell by regulating mRNA transport, splicing, editing, and stability. The RBP SRSF3 (serine/arginine-rich splicing factor 3) is essential for blastocyst formation and for proper liver development and function. However, its role in the heart has not been explored.
Objective:To investigate the role of SRSF3 in cardiac function.
Methods and Results: Cardiac SRSF3 expression was high at mid gestation and decreased during late embryonic development. Mice lacking SRSF3 in the embryonic heart showed impaired cardiomyocyte proliferation and died in utero. In the adult heart, SRSF3 expression was reduced after myocardial infarction, suggesting a possible role in cardiac homeostasis. To determine the role of this RBP in the adult heart, we used an inducible, cardiomyocyte-specific SRSF3 knockout mouse model. After SRSF3 depletion in cardiomyocytes, mice developed severe systolic dysfunction that resulted in death within 8 days. RNA-Seq analysis revealed downregulation of mRNAs encoding sarcomeric and calcium handling proteins. Cardiomyocyte-specific SRSF3 knockout mice also showed evidence of alternative splicing of mTOR (mammalian target of rapamycin) mRNA, generating a shorter protein isoform lacking catalytic activity. This was associated with decreased phosphorylation of 4E-BP1 (eIF4E-binding protein 1), a protein that binds to eIF4E (eukaryotic translation initiation factor 4E) and prevents mRNA decapping. Consequently, we found increased decapping of mRNAs encoding proteins involved in cardiac contraction. Decapping was partially reversed by mTOR activation.
Conclusions: We show that cardiomyocyte-specific loss of SRSF3 expression results in decapping of critical mRNAs involved in cardiac contraction. The molecular mechanism underlying this effect likely involves the generation of a short mTOR isoform by alternative splicing, resulting in reduced 4E-BP1 phosphorylation. The identification of mRNA decapping as a mechanism of systolic heart failure may open the way to the development of urgently needed therapeutic tools.
RATIONALE: RBPs (RNA-binding proteins) play critical roles in human biology and disease. Aberrant RBP expression affects various steps in RNA processing, altering the function of the target RNAs. The RBP SRSF4 (serine/arginine-rich splicing factor 4) has been linked to neuropathies and cancer. However, its role in the heart is completely unknown. OBJECTIVE: To investigate the role of SRSF4 in the heart. METHODS AND RESULTS: Echocardiography of mice specifically lacking SRSF4 in the heart (SRSF4 KO) revealed left ventricular hypertrophy and increased cardiomyocyte area, which led to progressive diastolic dysfunction with age. SRSF4 KO mice showed altered electrophysiological activity under isoproterenol-induced cardiac stress, with a post-QRS depression and a longer QT interval, indicating an elevated risk of sudden cardiac death. RNA-Seq analysis revealed expression changes in several long noncoding RNAs, including GAS5 (growth arrest-specific 5), which we identified as a direct SRSF4 target in cardiomyocytes by individual-nucleotide- resolution cross-linking and immuno-precipitation. GAS5 is a repressor of the GR (glucocorticoid receptor) and was downregulated in SRSF4 KO hearts. This corresponded with elevated GR transcriptional activity in cardiomyocytes, leading to increases in hypertrophy markers and cell size. Furthermore, hypertrophy in SRSF4 KO cardiomyocytes was reduced by overexpressing GAS5. CONCLUSIONS: Loss of SRSF4 expression results in cardiac hypertrophy, diastolic dysfunction, and abnormal repolarization. The molecular mechanism underlying this effect involves GAS5 downregulation and consequent elevation of GR transcriptional activity. Our findings may help to develop new therapeutic tools for the treatment of cardiac hypertrophy and myocardial pathology in patients with Cushing syndrome.
Kant, Piaget et Unityp
(1988)
Le livre de H. Seiler, "Apprehension. Language, Object and Order", présente un grand intérêt même pour und épistémologue ne disposant pas d'une formation de linguíste. A cela il y a au moins deux raísons: en premier lieu "Apprehension. Language, Object and Order" étudie la notion d'objet introduisant la DIMENSION de l'APPREHENSION et, en deuxième lieu, à travers l'étude des langues elle vise une universalité fonctionelle de l'activité cognitive. La notion d'objet est traditionellement importante pour toute recherche épistémologique et ces dernières années elle a été définitivement liée aux recherches sémantiques (Tugendhat 1976: 48). "Apprehension. Language, Object, and order" englobe cet aspect; en effet, le terme de APPREHENSION indique l'activité de saisie notionelle de l'objet telle qu'elle apparaît dans les langues. La structure des langues, mise en évidence dans cette DIMENSION de l'APPREHENSION, est considerée comme la manifestation (REPRAESENTATIO) d'un concept, le REPRAESENTANDUM. Dans notre cas, il s'agit du concept d'objet, dont la richesse esst détectable par la complexité de la REPRAESENTATIO línguistique, qui en met en évidence la nature fonctionelle. Mais sa nature polymorphe, apparaissant dans les TECHNIQUES de la DIMENSION, fait que la saisie due réel mise en oeuvre par ce concept ne pourra pas se reduire à une simple perception de l'objet. En developpant les recherches de "Apprehension. Language, Object and Order", on purra dépasser non seulement les conceptions de la sémantique fondées sur la notion d'adéquation (ou de satisfaction), mais aussi celle qui se réclament d'un 'jeu de vérification' (Tugendhat 1976: 265). Ces conceptions, loin de se vider de leur sens, seront intégrées dans un cadre plus général. En effet, la nature même de l'objet dépend, dans sa définition et dans sa saisie, de cette activité. Le dépassement de la notion d'adéquation amène à une reformulation de l'ontologie, que l'ensemble de "Apprehension. Language, Object and Order" suggère. Il faudra introduire, à mon avis, une conception constructiviste.