Refine
Year of publication
Document Type
- Article (132)
- Preprint (37)
- Working Paper (2)
- Contribution to a Periodical (1)
Has Fulltext
- yes (172)
Is part of the Bibliography
- no (172)
Keywords
- LHC (7)
- breast cancer (6)
- Acetogenesis (4)
- Bioenergetics (4)
- Wood-Ljungdahl pathway (4)
- ALICE (3)
- ALICE experiment (3)
- Acetogen (3)
- Biohydrogen (3)
- Hadron-Hadron Scattering (3)
- Hydrogenase (3)
- Thermoanaerobacter kivui (3)
- Thermophiles (3)
- Wood–Ljungdahl pathway (3)
- brain metastases (3)
- compatible solutes (3)
- hydrogen storage (3)
- pp collisions (3)
- Acetobacterium woodii (2)
- Acinetobacter baumannii (2)
- Anaerobes (2)
- Bacterial physiology (2)
- Beauty production (2)
- Biocatalysis (2)
- Breast cancer (2)
- Diagnostik (2)
- Electron Bifurcation (2)
- Electron Transfer (2)
- Electron Transport (2)
- Energy Conservation (2)
- Energy Metabolism (2)
- Energy conservation (2)
- Enzyme Mechanisms (2)
- Formate dehydrogenase (2)
- Früherkennung (2)
- Hydrogen production (2)
- Hydrogen-dependent CO2 reductase (2)
- Iron-Sulfur Protein (2)
- Mammakarzinom (2)
- Metabolism (2)
- Metalloenzymes (2)
- Nachsorge (2)
- Oxidoreductases (2)
- Richtlinie (2)
- Single electrons (2)
- Synthesis gas (2)
- acetogen (2)
- acetogenic bacteria (2)
- acetogens (2)
- carbon capture (2)
- desiccation (2)
- diagnosis (2)
- extremophile (2)
- fermentation (2)
- follow‑up (2)
- gene expression (2)
- guideline (2)
- hydrogen-dependent CO2 reductase (2)
- mannitol (2)
- screening (2)
- 900 GeV (1)
- ATP synthase (1)
- ATP synthesis (1)
- Acetobacterium (1)
- Acetogenic bacteria (1)
- Acetogenic metabolism (1)
- Anaerobic bacteria (1)
- Artland (1)
- BCOR (1)
- BCORL1 (1)
- Bacterial Metabolism (1)
- Bacterial genetics (1)
- Bacteriology (1)
- Bioenergetics/Electron Transfer Complex (1)
- Bioengineering (1)
- Biofuels (1)
- Bioreactor (1)
- Bone metastases (1)
- Brain metastasis (1)
- Butyrate (1)
- CO2 capture (1)
- CO2 reduction (1)
- Caffeate Respiration (1)
- Cancer treatment (1)
- Carbon capture (1)
- Carbon cycling (1)
- Cellular microbiology (1)
- Charm physics (1)
- Clinical genetics (1)
- Comparison with QCD (1)
- Cryoelectron microscopy (1)
- Cryoelectron tomography (1)
- DNA damage (1)
- Dark fermentation (1)
- Depression (1)
- Drought (1)
- E. coli (1)
- Ech (1)
- Electron transport chain (1)
- Electron-bifurcating hydrogenase (1)
- Enzyme Kinetics (1)
- Enzyme mechanisms (1)
- Eubacterium (1)
- Extended donor criteria (1)
- Extremophile (1)
- Extremophiles (1)
- Femtoscopy (1)
- Fiscal Policy (1)
- Flora (1)
- Frankfurt <Main> / Universität (1)
- Genome sequence (1)
- H+ transport (1)
- HBT (1)
- HER (1)
- Hadron production (1)
- Halobacillus halophilus (1)
- Heavy Ions (1)
- Heavy flavor production (1)
- Heavy flavour production (1)
- Heavy ions (1)
- Heavy-flavour production (1)
- Heavy-ion collisions (1)
- Heterologous enzyme production (1)
- Human behaviour (1)
- Hydrogen storage (1)
- Hydrogen-dependent CO2 reductase (HDCR) (1)
- Immunophenotyping (1)
- Inclusive spectra (1)
- Intensity interferometry (1)
- Jets (1)
- Lesions (1)
- Lower Saxony (1)
- Lymphocyte subpopulations (1)
- Macroeconomic Models (1)
- Mechanisms of disease (1)
- Membrane Energetics (1)
- Membrane Enzymes (1)
- Membrane Transport (1)
- MetVF (1)
- Methylene-THF reductase (1)
- Methylene-tetrahydrofolate reductase (1)
- Microbial ecology (1)
- Microbiology (1)
- Mid-rapidity (1)
- Mikrobiologie (1)
- Mikrobiologisches Institut (1)
- Model Comparison (1)
- Model Uncertainty (1)
- Monetary Policy (1)
- Multi-strange baryons (1)
- Multienzyme complexes (1)
- NK cell subsets (1)
- Na Gradient (1)
- Na+ transport (1)
- Necrosis (1)
- Non-small cell lung cancer (1)
- Nuclear modification factor (1)
- Optimization (1)
- Organ allocation (1)
- Osmostress (1)
- Oxidation-Reduction (1)
- PYTHIA (1)
- Pancreas transplantation (1)
- Pathogen (1)
- Pb–Pb (1)
- Persistence (1)
- Physiology (1)
- Policy Rules (1)
- Prediction of locoregional recurrence (1)
- Proton–proton (1)
- Pyrophosphatase (1)
- Radiochemotherapy (1)
- Radiotherapy (1)
- Red Data Book (1)
- Rejection (1)
- Relativistic heavy ion physics (1)
- Respiration (1)
- Rnf (1)
- Rnf complex (1)
- Robustness (1)
- SCCHN (1)
- Scale-up (1)
- Single muons (1)
- Sodium Transport (1)
- Sodium transport (1)
- Stereotactic radiosurgery (1)
- TGFB-induced factor homeobox 1 (1)
- TGIF (1)
- Thermophilic acetogenic bacteria (1)
- Toxicity (1)
- Transverse momentum (1)
- Virulence (1)
- X-ray crystallography (1)
- acetogenesis (1)
- acetogenic metabolism (1)
- acute myeloid leukemia (1)
- anaerobic metabolism (1)
- archaea (1)
- asymptomatic (1)
- bacteria (1)
- bacterial microcompartment (1)
- biocommodities (1)
- bioenergetics (1)
- bioengineering (1)
- biofuel (1)
- biofuels (1)
- biohydrogen (1)
- biophysics (1)
- biopsy (1)
- bioreactor (1)
- biotechnology (1)
- carbon dioxide reduction (1)
- carbon monoxide (1)
- cell biology (1)
- chemiosmosis (1)
- chloride accumulation (1)
- clostridia (1)
- cobaltocene (1)
- electron bifurcation (1)
- electron transport (1)
- energy conservation (1)
- energy-converting hydrogenase (Ech) (1)
- enzymatic electrosynthesis (1)
- enzyme activity (1)
- fiscal policy (1)
- formate dehydrogenase (1)
- formate oxidation (1)
- gene signature (1)
- genetic engineering (1)
- glutamine synthetase (1)
- halophile (1)
- head and neck squamous cell carcinoma (1)
- homologous gene expression (1)
- hybrid strategy (1)
- hydrogen evolution (1)
- hydrogen oxidation (1)
- hydrogen production (1)
- hydrogen-dependent carbon dioxide reductase (1)
- hydrogenase (1)
- hydrogenation of CO2 (1)
- imaging (1)
- kinetically trapped state (1)
- loss-of-function (1)
- macroeconomic models (1)
- mapping (1)
- membrane potential (1)
- methyltransferase (1)
- model comparison (1)
- model uncertainty (1)
- monetary policy (1)
- mouse (1)
- oocytes (1)
- osmoadaptation (1)
- osmoregulation (1)
- osmotic stress (1)
- p63 (1)
- papilloma (1)
- pattern (1)
- policy rules (1)
- postoperative radiochemotherapy (1)
- postoperative radiotherapy (1)
- prognostic scores (1)
- propanediol utilization (1)
- propensity score matching (1)
- protein production (1)
- proteoliposomes, (1)
- proton translocation (1)
- qH2 (1)
- quality control (1)
- radiological (1)
- redox polymer (1)
- regulation (1)
- respiratory chain (1)
- risk stratification (1)
- robustness (1)
- siphonograms (1)
- spectra (1)
- spring-loaded activation (1)
- structural biolog (1)
- survival (1)
- thermophiles (1)
- trehalose (1)
- value-added chemicals (1)
- whole-cell catalysis (1)
- √sN N = 2.76 TeV (1)
Institute
- Physik (81)
- Frankfurt Institute for Advanced Studies (FIAS) (69)
- Informatik (68)
- Biowissenschaften (63)
- Medizin (17)
- Biochemie und Chemie (6)
- Biochemie, Chemie und Pharmazie (3)
- Zentrum für Biomolekulare Magnetische Resonanz (BMRZ) (3)
- Exzellenzcluster Makromolekulare Komplexe (2)
- Georg-Speyer-Haus (2)
The ALICE Collaboration at the LHC reports measurement of the inclusive production cross section of electrons from semi-leptonic decays of beauty hadrons with rapidity |y| < 0.8 and transverse momentum 1 < pT < 10 GeV/c, in pp collisions at √s = 2.76 TeV. Electrons not originating from semi-electronic decay of beauty hadrons are suppressed using the impact parameter of the corresponding tracks. The production cross section of beauty decay electrons is compared to the result obtained with an alternative method which uses the distribution of the azimuthal angle between heavy-flavour decay electrons and charged hadrons. Perturbative QCD predictions agree with the measured cross section within the experimental and theoretical uncertainties. The integrated visible cross section, σb→e = 3.47 ± 0.40(stat) +1.12 −1.33(sys) ± 0.07(norm) μb, was extrapolated to full phase space using Fixed Order plus Next-to-Leading Log (FONLL) calculations to obtain the total bb production ¯ cross section, σbb¯ = 130 ± 15.1(stat) +42.1 −49.8(sys) +3.4 −3.1(extr) ± 2.5(norm) ± 4.4(BR) μb.
Synthesis of acetate from carbon dioxide and molecular hydrogen is considered to be the first carbon assimilation pathway on earth. It combines carbon dioxide fixation into acetyl-CoA with the production of ATP via an energized cell membrane. How the pathway is coupled with the net synthesis of ATP has been an enigma. The anaerobic, acetogenic bacterium Acetobacterium woodii uses an ancient version of this pathway without cytochromes and quinones. It generates a sodium ion potential across the cell membrane by the sodium-motive ferredoxin:NAD oxidoreductase (Rnf). The genome sequence of A. woodii solves the enigma: it uncovers Rnf as the only ion-motive enzyme coupled to the pathway and unravels a metabolism designed to produce reduced ferredoxin and overcome energetic barriers by virtue of electron-bifurcating, soluble enzymes.
Transverse momentum spectra of π±, K± and p(p¯) up to pT = 20 GeV/c at mid-rapidity in pp, peripheral (60–80%) and central (0–5%) Pb–Pb collisions at √sNN = 2.76 TeV have been measured using the ALICE detector at the Large Hadron Collider. The proton-to-pion and the kaon-to-pion ratios both show a distinct peak at pT ≈ 3 GeV/c in central Pb–Pb collisions. Below the peak, pT < 3 GeV/c, both ratios are in good agreement with hydrodynamical calculations, suggesting that the peak itself is dominantly the result of radial flow rather than anomalous hadronization processes. For pT > 10 GeV/c particle ratios in pp and Pb–Pb collisions are in agreement and the nuclear modification factors for π±, K± and p(p¯) indicate that, within the systematic and statistical uncertainties, the suppression is the same. This suggests that the chemical composition of leading particles from jets in the medium is similar to that of vacuum jets.
Acetobacterium woodii utilizes the Wood‐Ljungdahl pathway for reductive synthesis of acetate from carbon dioxide. However, A. woodii can also perform non‐acetogenic growth on 1,2‐propanediol (1,2‐PD) where instead of acetate, equal amounts of propionate and propanol are produced as metabolic end products. Metabolism of 1,2‐PD occurs via encapsulated metabolic enzymes within large proteinaceous bodies called bacterial microcompartments. While the genome of A. woodii harbours 11 genes encoding putative alcohol dehydrogenases, the BMC‐encapsulated propanol‐generating alcohol dehydrogenase remains unidentified. Here, we show that Adh4 of A. woodii is the alcohol dehydrogenase required for propanol/ethanol formation within these microcompartments. It catalyses the NADH‐dependent reduction of propionaldehyde or acetaldehyde to propanol or ethanol and primarily functions to recycle NADH within the BMC. Removal of adh4 gene from the A. woodii genome resulted in slow growth on 1,2‐PD and the mutant displayed reduced propanol and enhanced propionate formation as a metabolic end product. In sum, the data suggest that Adh4 is responsible for propanol formation within the BMC and is involved in redox balancing in the acetogen, A. woodii.
The acetogenic model bacterium Acetobacterium woodii is well-known to produce acetate by homoacetogenesis from sugars, but under certain conditions minor amounts of ethanol are produced in addition. Here, we have aimed to identify physiological conditions that increase electron and carbon flow towards ethanol production. Ethanol was only produced from fructose but not from H2 + CO2, formate, pyruvate, lactate or alanine. In the absence of Na+, the Wood–Ljungdahl pathway (WLP) of acetate formation is not functional. Therefore, the ethanol yield increased to 0.42 mol/mol (ethanol/fructose) with an ethanol/acetate ratio of 0.28 mol/mol. The presence of bicarbonate/CO2 stimulated electron and carbon flow through the WLP and led to less ethanol produced. Of the 11 potential alcohol dehydrogenase genes, the most upregulated during ethanologenesis was adh4. A deletion of adh4 led to an increase in ethanol production by 100% to a yield of 0.79 mol/mol (ethanol/fructose); this correlated with an increase in transcript abundance of adh6. In sum, our studies revealed low Na+ and bicarbonate/CO2 as factors that trigger ethanol formation and that a deletion of adh4 drastically increased ethanol formation in A. woodii.
More than 2 million tons of glycerol are produced during industrial processes each year and, therefore, glycerol is an inexpensive feedstock to produce biocommodities by bacterial fermentation. Acetogenic bacteria are interesting production platforms and there have been few reports in the literature on glycerol utilization by this ecophysiologically important group of strictly anaerobic bacteria. Here, we show that the model acetogen Acetobacterium woodii DSM1030 is able to grow on glycerol, but contrary to expectations, only for 2–3 transfers. Transcriptome analysis revealed the expression of the pdu operon encoding a propanediol dehydratase along with genes encoding bacterial microcompartments. Deletion of pduAB led to a stable growth of A. woodii on glycerol, consistent with the hypothesis that the propanediol dehydratase also acts on glycerol leading to a toxic end-product. Glycerol is oxidized to acetate and the reducing equivalents are reoxidized by reducing CO2 in the Wood–Ljungdahl pathway, leading to an additional acetate. The possible oxidation product of glycerol, dihydroxyacetone (DHA), also served as carbon and energy source for A. woodii and growth was stably maintained on that compound. DHA oxidation was also coupled to CO2 reduction. Based on transcriptome data and enzymatic analysis we present the first metabolic and bioenergetic schemes for glycerol and DHA utilization in A. woodii.
Interspecies hydrogen transfer in anoxic ecosystems is essential for the complete microbial breakdown of organic matter to methane. Acetogenic bacteria are key players in anaerobic food webs and have been considered as prime candidates for hydrogen cycling. We have tested this hypothesis by mutational analysis of the hydrogenase in the model acetogen Acetobacterium woodii. Hydrogenase-deletion mutants no longer grew on H2 + CO2 or organic substrates such as fructose, lactate, or ethanol. Heterotrophic growth could be restored by addition of molecular hydrogen to the culture, indicating that hydrogen is an intermediate in heterotrophic growth. Indeed, hydrogen production from fructose was detected in a stirred-tank reactor. The mutant grew well on organic substrates plus caffeate, an alternative electron acceptor that does not require molecular hydrogen but NADH as reductant. These data are consistent with the notion that molecular hydrogen is produced from organic substrates and then used as reductant for CO2 reduction. Surprisingly, hydrogen cycling in A. woodii is different from the known modes of interspecies or intraspecies hydrogen cycling. Our data are consistent with a novel type of hydrogen cycling that connects an oxidative and reductive metabolic module in one bacterial cell, "intracellular syntrophy."
We report on the production of inclusive Υ (1S) and Υ (2S) in p–Pb collisions at √sNN = 5.02 TeV at the LHC. The measurement is performed with the ALICE detector at backward (−4.46 < ycms < −2.96) and forward (2.03 .< ycms < 3.53) rapidity down to zero transverse momentum. The production cross sections of the Υ (1S) and Υ (2S) are presented, as well as the nuclear modification factor and the ratio of the forward to backward yields of Υ (1S). A suppression of the inclusive Υ (1S) yield in p–Pb collisions with respect to the yield from pp collisions scaled by the number of binary nucleon–nucleon collisions is observed at forward rapidity but not at backward rapidity. The results are compared to theoretical model calculations including nuclear shadowing or partonic energy loss effect.
Energy-conserving dimethyl sulfoxide reduction in the acetogenic bacterium Moorella thermoacetica
(2022)
Moorella thermoacetica is one of the well-studied thermophilic acetogenic bacteria. It grows by oxidation of organic substrates, CO or H2 coupled to CO2 reduction to acetate. Here, we describe that M. thermoacetica can also use dimethyl sulfoxide as terminal electron acceptor. Growth of M. thermoacetica on glucose or H2 + CO2 was stimulated by dimethyl sulfoxide (DMSO). Membranes showed a DMSO reductase activity, that was induced by growing cells in presence of DMSO. The enzyme used reduced anthraquinone-2,6-disulfonate, benzyl- and methyl viologen as electron donor, but not NAD(P)H. Activity was highest at pH 5 and 60°C, the Km for DMSO was 2.4 mM. Potential DMSO reductase subunits were identified by peptide mass fingerprinting; they are encoded in a genomic region that contains three potential dmsA genes, three dmsB genes and one dmsC gene. Transcriptome analysis revealed that two different dmsAB gene clusters were induced in the presence of DMSO. The function of these two and their predicted biochemical features are discussed. In addition, the data are in line with the hypothesis that M. thermoacetica can use DMSO alongside CO2 as electron acceptor and DMSO reduction is catalysed by an energy-conserving, membrane-bound electron transport chain with DMSO as final electron acceptor.
Acetogenic bacteria are already established as biocatalysts for production of high-value compounds from C1 substrates such as H2 + CO2 or CO. However, little is known about the physiology, biochemistry and bioenergetics of acetogenesis from formate, an interesting feedstock for biorefineries. Here, we analysed formate metabolism in the model acetogen Acetobacterium woodii. Cells grew optimally on 200 mM formate to an optical density of 0.6. Formate was exclusively converted to acetate (and CO2) with a ratio of 4.4:1. Transcriptome analyses revealed genes/enzymes involved in formate metabolism. Strikingly, A. woodii has two genes potentially encoding a formyl-THF synthetase, fhs1 and fhs2. fhs2 forms an operon with a gene encoding a potential formate transporter, fdhC. Deletion of fhs2/fdhC led to a reduced growth rate, formate consumption and optical densities. Acetogenesis from H2 + CO2 was accompanied by transient formate production; strikingly, formate reutilization was completely abolished in the Δfhs2/fdhC mutant. Take together, our studies gave the first detailed insights into the formatotrophic lifestyle of A. woodii.