Cristae architecture is determined by an interplay of the MICOS complex and the F1FO ATP synthase via Mic27 and Mic10

  • The inner boundary and the cristae membrane are connected by pore-like structures termed crista junctions (CJs). The MICOS complex is required for CJ formation and enriched at CJs. Here, we address the roles of the MICOS subunits Mic27 and Mic10. We observe a positive genetic interaction between Mic27 and Mic60 and deletion of Mic27 results in impaired formation of CJs and altered cristae membrane curvature. Mic27 acts in an antagonistic manner to Mic60 as it promotes oligomerization of the F1FO-ATP synthase and partially restores CJ formation in cells lacking Mic60. Mic10 impairs oligomerization of the F1FO-ATP synthase similar to Mic60. Applying complexome profiling, we observed that deletion of Mic27 destabilizes the MICOS complex but does not impair formation of a high molecular weight Mic10 subcomplex. Moreover, this Mic10 subcomplex comigrates with the dimeric F1FO-ATP synthase in a Mic27-independent manner. Further, we observed a chemical crosslink of Mic10 to Mic27 and of Mic10 to the F1FO-ATP synthase subunit e. We corroborate the physical interaction of the MICOS complex and the F1FO-ATP synthase. We propose a model in which part of the F1FO-ATP synthase is linked to the MICOS complex via Mic10 and Mic27 and by that is regulating CJ formation.
Metadaten
Author:Katharina Eydt, Karen M. Davies, Christina Behrendt, Ilka WittigORCiD, Andreas S. Reichert
URN:urn:nbn:de:hebis:30:3-445550
DOI:https://doi.org/10.15698/mic2017.08.585
ISSN:2311-2638
Pubmed Id:https://pubmed.ncbi.nlm.nih.gov/28845423
Parent Title (English):Microbial cell
Publisher:Shared Science Publ.
Place of publication:Graz
Document Type:Article
Language:English
Year of Completion:2017
Date of first Publication:2017/07/20
Publishing Institution:Universit├Ątsbibliothek Johann Christian Senckenberg
Release Date:2017/12/12
Tag:bioenergetics; crista junction; cristae; membrane protein complex; membrane structure; mitochondria
Volume:4
Issue:8
Page Number:14
First Page:259
Last Page:272
Note:
ristae architecture is determined by an interplay of the MICOS complex and the F1FO ATP synthase via Mic27 and Mic10 by Eydt et al. is licensed under a Creative Commons Attribution 4.0 International License.
HeBIS-PPN:428648517
Institutes:Medizin / Medizin
Exzellenzcluster / Exzellenzcluster Makromolekulare Komplexe
Angeschlossene und kooperierende Institutionen / MPI f├╝r Biophysik
Dewey Decimal Classification:5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie
Sammlungen:Universit├Ątspublikationen
Licence (German):License LogoCreative Commons - Namensnennung 4.0