Multi-endpoint toxicological assessment of polystyrene nano- and microparticles in different biological models in vitro

  • Nanoplastics (NP) and microplastics (MP) accumulate in our environment as a consequence of the massive consumption of plastics. Huge knowledge-gaps exist regarding uptake and fate of plastic particles in micro- and nano-dimensions in humans as well as on their impact on human health. This study investigated the transport and effects of 50 nm and 0.5 μm COOH-modified polystyrene (PS) particles, as representatives for NP and MP, in different biological models in vitro. Acute toxicity and potential translocation of the particles were studied at the human intestinal and placental barrier using advanced in vitro co-culture models. Furthermore, embryotoxicity and genotoxicity were investigated as highly sensitive endpoints. Polystyrene was not acutely toxic in both sizes (nano- and microparticles). No transport across the intestinal and placental barrier but a cellular uptake and intracellular accumulation of PS nano- and microparticles were determined. The particles were identified as weak embryotoxic and non-genotoxic. In contrast to single-organ studies, this multi-endpoint study is providing a data-set with the exact same type of particles to compare organ-specific outcomes. Our study clearly shows the need to investigate other types of plastics as well as towards long-term or chronic effects of plastic particles in different biological models in vitro.
Author:Michelle Hesler, Leonie Aengenheister, Bernhard Ellinger, Roland Drexel, Susanne Straskraba, Carsten Jost, Sylvia Wagner, Florian Meier, Hagen von Briesen, Claudia BüchelORCiD, Peter Wick, Tina Bürki-Thurnherr, Yvonne Kohl
Pubmed Id:
Parent Title (English):Toxicology in vitro
Publisher:Elsevier Science
Place of publication:Amsterdam [u. a.]
Document Type:Article
Year of Completion:2019
Date of first Publication:2019/07/27
Publishing Institution:Universitätsbibliothek Johann Christian Senckenberg
Release Date:2019/09/05
Tag:Embryotoxicity; Intestinal barrier; Multi-endpoint toxicity study; Nano- and microplastics; Placental barrier; Polystyrene
Issue:Art. 104610
Page Number:15
First Page:1
Last Page:15
© 2019 The Authors. Published by Elsevier Ltd. This is an open access article under the CC BY-NC-ND license (
Institutes:Biowissenschaften / Biowissenschaften
Dewey Decimal Classification:5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie
Licence (German):License LogoCreative Commons - Namensnennung-Nicht kommerziell - Keine Bearbeitung 4.0