Şebnem Hazal Gülşen, Evren Tileklioglu, Edna Bode, Harun Cimen, Hatice Ertabaklar, Derya Uluğ, Sema Ertuğ, Sebastian Leonhard Wenski, Mustapha Touray, Canan Hazir, Duygu Kaya Bilecenoglu, Ibrahim Yildiz, Helge Björn Bode, Selcuk Hazir
- Natural products have been proven to be important starting points for the development of new drugs. Bacteria in the genera Photorhabdus and Xenorhabdus produce antimicrobial compounds as secondary metabolites to compete with other organisms. Our study is the first comprehensive study screening the anti-protozoal activity of supernatants containing secondary metabolites produced by 5 Photorhabdus and 22 Xenorhabdus species against human parasitic protozoa, Acanthamoeba castellanii, Entamoeba histolytica, Trichomonas vaginalis, Leishmania tropica and Trypanosoma cruzi, and the identification of novel bioactive antiprotozoal compounds using the easyPACId approach (easy Promoter Activated Compound Identification) method. Though not in all species, both bacterial genera produce antiprotozoal compounds effective on human pathogenic protozoa. The promoter exchange mutants revealed that antiprotozoal bioactive compounds produced by Xenorhabdus bacteria were fabclavines, xenocoumacins, xenorhabdins and PAX peptides. Among the bacteria assessed, only P. namnaoensis appears to have acquired amoebicidal property which is effective on E. histolytica trophozoites. These discovered antiprotozoal compounds might serve as starting points for the development of alternative and novel pharmaceutical agents against human parasitic protozoa in the future.
MetadatenAuthor: | Şebnem Hazal GülşenORCiD, Evren TilekliogluORCiD, Edna BodeORCiDGND, Harun CimenORCiD, Hatice ErtabaklarORCiD, Derya UluğORCiD, Sema ErtuğORCiD, Sebastian Leonhard WenskiGND, Mustapha TourayORCiD, Canan HazirORCiD, Duygu Kaya BilecenogluORCiD, Ibrahim YildizORCiD, Helge Björn BodeORCiDGND, Selcuk HazirORCiD |
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URN: | urn:nbn:de:hebis:30:3-695612 |
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DOI: | https://doi.org/10.1038/s41598-022-13722-z |
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ISSN: | 2045-2322 |
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Parent Title (English): | Scientific reports |
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Publisher: | Macmillan Publishers Limited, part of Springer Nature |
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Place of publication: | [London] |
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Document Type: | Article |
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Language: | English |
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Date of Publication (online): | 2022/06/24 |
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Date of first Publication: | 2022/06/24 |
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Publishing Institution: | Universitätsbibliothek Johann Christian Senckenberg |
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Release Date: | 2023/08/11 |
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Tag: | Biotechnology; Drug discovery; Microbiology; Molecular biology; Pathogenesis |
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Volume: | 12 |
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Issue: | art. 10779 |
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Article Number: | 10779 |
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Page Number: | 13 |
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First Page: | 1 |
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Last Page: | 13 |
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Note: | Open Access funding enabled and organized by Projekt DEAL. This study was supported by the Scientific and Technical Research Council of Turkey (TUBITAK-Project Number: 116S387) and Aydin Adnan Menderes University, Project Number: 20001). |
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HeBIS-PPN: | 512760586 |
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Institutes: | Biowissenschaften |
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| Angeschlossene und kooperierende Institutionen / Senckenbergische Naturforschende Gesellschaft |
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Dewey Decimal Classification: | 5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie |
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Sammlungen: | Universitätspublikationen |
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Licence (German): | Creative Commons - CC BY - Namensnennung 4.0 International |
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