Neuroligin-3 regulates excitatory synaptic transmission and EPSP-spike coupling in the dentate gyrus in vivo
- Neuroligin-3 (Nlgn3), a neuronal adhesion protein implicated in autism spectrum disorder (ASD), is expressed at excitatory and inhibitory postsynapses and hence may regulate neuronal excitation/inhibition balance. To test this hypothesis, we recorded field excitatory postsynaptic potentials (fEPSPs) in the dentate gyrus of Nlgn3 knockout (KO) and wild-type mice. Synaptic transmission evoked by perforant path stimulation was reduced in KO mice, but coupling of the fEPSP to the population spike was increased, suggesting a compensatory change in granule cell excitability. These findings closely resemble those in neuroligin-1 (Nlgn1) KO mice and could be partially explained by the reduction in Nlgn1 levels we observed in hippocampal synaptosomes from Nlgn3 KO mice. However, unlike Nlgn1, Nlgn3 is not necessary for long-term potentiation. We conclude that while Nlgn1 and Nlgn3 have distinct functions, both are required for intact synaptic transmission in the mouse dentate gyrus. Our results indicate that interactions between neuroligins may play an important role in regulating synaptic transmission and that ASD-related neuroligin mutations may also affect the synaptic availability of other neuroligins.
Author: | Julia MuellerleileORCiDGND, Matej VnenčákGND, Angelo IppolitoORCiDGND, Dilja Krueger-BurgORCiD, Tassilo JungenitzORCiDGND, Stephan SchwarzacherORCiDGND, Peter JedličkaORCiDGND |
---|---|
URN: | urn:nbn:de:hebis:30:3-695784 |
DOI: | https://doi.org/10.1007/s12035-021-02663-9 |
ISSN: | 1559-1182 |
Parent Title (English): | Molecular neurobiology |
Publisher: | Humana Press |
Place of publication: | Totowa, NJ |
Document Type: | Article |
Language: | English |
Date of Publication (online): | 2021/11/29 |
Date of first Publication: | 2021/11/29 |
Publishing Institution: | Universitätsbibliothek Johann Christian Senckenberg |
Release Date: | 2023/09/22 |
Tag: | Autism Spectrum disorder; In vivo electrophysiology; Neuroligins; Synaptic transmission; Synaptosomal preparation |
Volume: | 59 |
Issue: | 2 |
Page Number: | 14 |
First Page: | 1098 |
Last Page: | 1111 |
Note: | Open Access funding enabled and organized by Projekt DEAL. |
HeBIS-PPN: | 513145915 |
Institutes: | Biowissenschaften |
Medizin | |
Dewey Decimal Classification: | 5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie |
6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit | |
Sammlungen: | Universitätspublikationen |
Licence (German): | Creative Commons - CC BY - Namensnennung 4.0 International |