Bestimmung des Molekulargewichtes der 20-β-Hydroxy-steroid-dehydrogenase
- The enzyme 20-β-Hydroxy-steroid-dehydrogenase obtained from the culture of Streptomyces hydrogenans and dissolved in 0.05 M Tris puffer, pH 7.3, has been investigated by means of a ultracentrifuge at 20 °C. The sedimentation- as well as the diffusion-coefficients obtained from various solutions at different concentrations were extrapolated to the concentration c = 0. The resulting zero-value for the sedimentation coefficient is s0 = 6.64 s and for the diffusion coefficient is D0 = 5.51 × 10-7 cm2/sec. Supposing the partial specific volume of the enzyme under consideration analogously to other similar proteins is V+=0.749 ml/g, the molecular weight has been estimated as M = 118 400.
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| Author: | Matatiahu Gehatia |
|---|---|
| URN: | urn:nbn:de:hebis:30:3-698903 |
| DOI: | https://doi.org/10.1515/znb-1962-0704 |
| ISSN: | 1865-7117 |
| ISSN: | 0932-0776 |
| Parent Title (German): | Zeitschrift für Naturforschung, B |
| Publisher: | Verlag der Zeitschrift für Naturforschung |
| Place of publication: | Tübingen |
| Document Type: | Article |
| Language: | German |
| Date of Publication (online): | 2014/06/02 |
| Year of first Publication: | 1962 |
| Publishing Institution: | Universitätsbibliothek Johann Christian Senckenberg |
| Release Date: | 2023/07/07 |
| Volume: | 17 |
| Issue: | 7 |
| Page Number: | 5 |
| First Page: | 432 |
| Last Page: | 436 |
| HeBIS-PPN: | 512276153 |
| Institutes: | Biochemie, Chemie und Pharmazie / Biochemie und Chemie |
| Dewey Decimal Classification: | 5 Naturwissenschaften und Mathematik / 54 Chemie / 540 Chemie und zugeordnete Wissenschaften |
| Sammlungen: | Universitätspublikationen |
| Licence (German): |  Creative Commons - Namensnennung-Nicht kommerziell-Keine Bearbeitung 3.0 |
