Cytochrome c oxidase biogenesis and metallochaperone interactions : steps in the assembly pathway of a bacterial complex
- Biogenesis of mitochondrial cytochrome c oxidase (COX) is a complex process involving the coordinate expression and assembly of numerous subunits (SU) of dual genetic origin. Moreover, several auxiliary factors are required to recruit and insert the redox-active metal compounds, which in most cases are buried in their protein scaffold deep inside the membrane. Here we used a combination of gel electrophoresis and pull-down assay techniques in conjunction with immunostaining as well as complexome profiling to identify and analyze the composition of assembly intermediates in solubilized membranes of the bacterium Paracoccus denitrificans. Our results show that the central SUI passes through at least three intermediate complexes with distinct subunit and cofactor composition before formation of the holoenzyme and its subsequent integration into supercomplexes. We propose a model for COX biogenesis in which maturation of newly translated COX SUI is initially assisted by CtaG, a chaperone implicated in CuB site metallation, followed by the interaction with the heme chaperone Surf1c to populate the redox-active metal-heme centers in SUI. Only then the remaining smaller subunits are recruited to form the mature enzyme which ultimately associates with respiratory complexes I and III into supercomplexes.
Author: | Sonja SchimoGND, Ilka WittigORCiD, Klaas Martinus PosORCiD, Bernd LudwigGND |
---|---|
URN: | urn:nbn:de:hebis:30:3-428863 |
URL: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5249081 |
DOI: | https://doi.org/10.1371/journal.pone.0170037 |
ISSN: | 1932-6203 |
Pubmed Id: | https://pubmed.ncbi.nlm.nih.gov/28107462 |
Parent Title (English): | PLoS one |
Publisher: | PLoS |
Place of publication: | Lawrence, Kan. |
Contributor(s): | Alessandro Giuffrè |
Document Type: | Article |
Language: | English |
Date of Publication (online): | 2017/02/23 |
Date of first Publication: | 2017/01/20 |
Publishing Institution: | Universitätsbibliothek Johann Christian Senckenberg |
Release Date: | 2017/02/23 |
Volume: | 12 |
Issue: | (1): e0170037 |
Page Number: | 19 |
First Page: | 1 |
Last Page: | 19 |
Note: | Copyright: © 2017 Schimo et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
HeBIS-PPN: | 448152371 |
Institutes: | Biochemie, Chemie und Pharmazie / Biochemie und Chemie |
Medizin / Medizin | |
Exzellenzcluster / Exzellenzcluster Makromolekulare Komplexe | |
Wissenschaftliche Zentren und koordinierte Programme / Sonderforschungsbereiche / Forschungskollegs | |
Dewey Decimal Classification: | 5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie |
Sammlungen: | Universitätspublikationen |
Licence (German): | Creative Commons - Namensnennung 4.0 |