Carolin Hacker, Nina A. Christ, Elke Duchardt-Ferner, Sophie Korn, Christoph Göbl, Lucija Berninger, Stefanie Düsterhus, Ute Hellmich, Tobias Madl, Peter Kötter, Karl-Dieter Entian, Jens Wöhnert
- Many Gram-positive bacteria produce lantibiotics, genetically encoded and posttranslationally modified peptide antibiotics, which inhibit the growth of other Gram-positive bacteria. To protect themselves against their own lantibiotics these bacteria express a variety of immunity proteins including the LanI lipoproteins. The structural and mechanistic basis for LanI-mediated lantibiotic immunity is not yet understood. Lactococcus lactis produces the lantibiotic nisin, which is widely used as a food preservative. Its LanI protein NisI provides immunity against nisin but not against structurally very similar lantibiotics from other species such as subtilin from Bacillus subtilis. To understand the structural basis for LanI-mediated immunity and their specificity we investigated the structure of NisI. We found that NisI is a two-domain protein. Surprisingly, each of the two NisI domains has the same structure as the LanI protein from B. subtilis, SpaI, despite the lack of significant sequence homology. The two NisI domains and SpaI differ strongly in their surface properties and function. Additionally, SpaI-mediated lantibiotic immunity depends on the presence of a basic unstructured N-terminal region that tethers SpaI to the membrane. Such a region is absent from NisI. Instead, the N-terminal domain of NisI interacts with membranes but not with nisin. In contrast, the C-terminal domain specifically binds nisin and modulates the membrane affinity of the N-terminal domain. Thus, our results reveal an unexpected structural relationship between NisI and SpaI and shed light on the structural basis for LanI mediated lantibiotic immunity.
MetadatenAuthor: | Carolin HackerORCiDGND, Nina A. ChristGND, Elke Duchardt-FernerORCiD, Sophie KornORCiDGND, Christoph GöblORCiD, Lucija BerningerGND, Stefanie Düsterhus, Ute HellmichORCiDGND, Tobias MadlORCiD, Peter KötterORCiD, Karl-Dieter Entian, Jens WöhnertORCiDGND |
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URN: | urn:nbn:de:hebis:30:3-772074 |
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DOI: | https://doi.org/10.1074/jbc.M115.679969 |
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ISSN: | 0021-9258 |
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Pubmed Id: | https://pubmed.ncbi.nlm.nih.gov/26459561 |
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Parent Title (English): | Journal of biological chemistry |
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Publisher: | American Society for Biochemistry and Molecular Biology Publications |
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Place of publication: | Bethesda, Md |
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Document Type: | Article |
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Language: | English |
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Date of Publication (online): | 2021/01/04 |
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Year of first Publication: | 2015 |
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Publishing Institution: | Universitätsbibliothek Johann Christian Senckenberg |
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Release Date: | 2024/03/14 |
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Tag: | antibiotic resistance; antibiotics; lantibiotic; lipoprotein; nisin binding; nuclear magnetic resonance (NMR); protein structure; small angle x-ray scattering |
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Volume: | 290.2015 |
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Issue: | 48 |
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Page Number: | 18 |
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First Page: | 28869 |
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Last Page: | 28886 |
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Institutes: | Biowissenschaften |
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| Biochemie, Chemie und Pharmazie / Biochemie und Chemie |
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Dewey Decimal Classification: | 5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie |
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Sammlungen: | Universitätspublikationen |
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Licence (German): | Creative Commons - CC BY - Namensnennung 4.0 International |
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