Arnau Casanas, Rangana Warshamanage, Aaron D. Finke, Ezequiel Panepucci, Vincent Olieric, Anne Nöll, Robert Tampé, Stefan Brandstetter, Andreas Förster, Marcus Mueller, Clemens Schulze-Briese, Oliver Bunk, Meitian Wang
- The development of single-photon-counting detectors, such as the PILATUS, has been a major recent breakthrough in macromolecular crystallography, enabling noise-free detection and novel data-acquisition modes. The new EIGER detector features a pixel size of 75 × 75 µm, frame rates of up to 3000 Hz and a dead time as low as 3.8 µs. An EIGER 1M and EIGER 16M were tested on Swiss Light Source beamlines X10SA and X06SA for their application in macromolecular crystallography. The combination of fast frame rates and a very short dead time allows high-quality data acquisition in a shorter time. The ultrafine φ-slicing data-collection method is introduced and validated and its application in finding the optimal rotation angle, a suitable rotation speed and a sufficient X-ray dose are presented. An improvement of the data quality up to slicing at one tenth of the mosaicity has been observed, which is much finer than expected based on previous findings. The influence of key data-collection parameters on data quality is discussed.
MetadatenAuthor: | Arnau Casanas, Rangana WarshamanageORCiD, Aaron D. Finke, Ezequiel PanepucciORCiD, Vincent OliericORCiDGND, Anne Nöll, Robert TampéORCiDGND, Stefan BrandstetterORCiDGND, Andreas Förster, Marcus Mueller, Clemens Schulze-BrieseORCiD, Oliver BunkORCiD, Meitian WangORCiD |
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URN: | urn:nbn:de:hebis:30:3-428850 |
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URL: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5013597 |
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DOI: | https://doi.org/10.1107/S2059798316012304 |
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ISSN: | 2059-7983 |
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ISSN: | 1399-0047 |
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ISSN: | 0907-4449 |
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Pubmed Id: | https://pubmed.ncbi.nlm.nih.gov/27599736 |
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Parent Title (German): | Acta crystallographica. Section D, Biological crystallography online |
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Publisher: | Wiley-Blackwell |
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Place of publication: | Oxford |
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Contributor(s): | Kay Diederichs |
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Document Type: | Article |
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Language: | English |
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Date of Publication (online): | 2017/02/23 |
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Date of first Publication: | 2016/06/29 |
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Publishing Institution: | Universitätsbibliothek Johann Christian Senckenberg |
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Release Date: | 2017/02/23 |
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Tag: | EIGER detector; X-ray detectors; data-collection strategy; macromolecular crystallography |
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Volume: | 72 |
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Issue: | Pt 9 |
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Page Number: | 13 |
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First Page: | 1036 |
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Last Page: | 1048 |
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Note: | This is an open-access article distributed under the terms of the Creative Commons Attribution (CC-BY) Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original authors and source are cited. |
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HeBIS-PPN: | 451938313 |
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Institutes: | Biochemie, Chemie und Pharmazie / Biochemie und Chemie |
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| Wissenschaftliche Zentren und koordinierte Programme / Center for Membrane Proteomics (CMP) |
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| Wissenschaftliche Zentren und koordinierte Programme / Sonderforschungsbereiche / Forschungskollegs |
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Dewey Decimal Classification: | 5 Naturwissenschaften und Mathematik / 54 Chemie / 540 Chemie und zugeordnete Wissenschaften |
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Sammlungen: | Universitätspublikationen |
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Licence (German): | Creative Commons - Namensnennung 2.0 |
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