Novel strategies to improve co-fermentation of pentoses with D-glucose by recombinant yeast strains in lignocellulosic hydrolysates
- Economically feasible production of second-generation biofuels requires efficient co-fermentation of pentose and hexose sugars in lignocellulosic hydrolysates under very harsh conditions. Baker’s yeast is an excellent, traditionally used ethanol producer but is naturally not able to utilize pentoses. This is due to the lack of pentose-specific transporter proteins and enzymatic reactions. Thus, natural yeast strains must be modified by genetic engineering. Although the construction of various recombinant yeast strains able to ferment pentose sugars has been described during the last two decades, their rates of pentose utilization is still significantly lower than D-glucose fermentation. Moreover, pentoses are only fermented after D-glucose is exhausted, resulting in an uneconomical increase in the fermentation time. In this addendum, we discuss novel approaches to improve utilization of pentoses by development of specific transporters and substrate channeling in enzyme cascades. Addendum to: T Subtil, E Boles. Competition between pentoses and glucose during uptake and catabolism in recombinant Saccharomyces cerevisiae. Biotechnol Biofuels 2012; 5: 14 PMID: 22424089 DOI: 10.1186/1754-6834-5-14
Author: | Igor-Mislav OrebORCiDGND, Heiko Dietz, Alexander Farwick, Eckhard BolesORCiD |
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URN: | urn:nbn:de:hebis:30:3-288612 |
DOI: | https://doi.org/10.4161/bioe.21444 |
Pubmed Id: | https://pubmed.ncbi.nlm.nih.gov/22892590 |
Parent Title (English): | Bioengineered |
Publisher: | Landes Bioscience |
Place of publication: | Austin, Tex. |
Document Type: | Article |
Language: | English |
Year of Completion: | 2012 |
Year of first Publication: | 2012 |
Publishing Institution: | Universitätsbibliothek Johann Christian Senckenberg |
Release Date: | 2013/02/14 |
Tag: | bioethanol; co-fermentation of pentoses with glucose; substrate channeling; sugar transport |
Volume: | 3 |
Issue: | 6 |
Page Number: | 5 |
First Page: | 347 |
Last Page: | 351 |
Note: | Copyright © 2012 Landes Bioscience. This is an open-access article licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. The article may be redistributed, reproduced, and reused for non-commercial purposes, provided the original source is properly cited. |
HeBIS-PPN: | 331489473 |
Institutes: | Biowissenschaften / Biowissenschaften |
Dewey Decimal Classification: | 5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie |
Sammlungen: | Universitätspublikationen |
Sammlung Biologie / Sondersammelgebiets-Volltexte | |
Licence (German): | Creative Commons - Namensnennung-Nicht kommerziell 3.0 |