Bleaching-independent, whole-cell, 3D and multi-color STED imaging with exchangeable fluorophores
- We demonstrate bleaching-independent STED microscopy using fluorogenic labels that reversibly bind to their target structure. A constant exchange of labels guarantees the removal of photobleached fluorophores and their replacement by intact fluorophores, thereby circumventing bleaching-related limitations of STED super-resolution imaging in fixed and living cells. Foremost, we achieve a constant labeling density and demonstrate a fluorescence signal for long and theoretically unlimited acquisition times. Using this concept, we demonstrate whole-cell, 3D, multi-color and live cell STED microscopy with up to 100 min acquisition time.
Author: | Christoph Klaus SpahnORCiDGND, Jonathan B. GrimmORCiD, Luke D. LavisORCiD, Marko LampeORCiDGND, Mike HeilemannORCiDGND |
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URN: | urn:nbn:de:hebis:30:3-725009 |
DOI: | https://doi.org/10.1101/420638 |
Parent Title (English): | bioRxiv |
Document Type: | Preprint |
Language: | English |
Date of Publication (online): | 2018/09/18 |
Date of first Publication: | 2018/09/18 |
Publishing Institution: | Universitätsbibliothek Johann Christian Senckenberg |
Release Date: | 2023/07/26 |
Issue: | 420638 |
Page Number: | 7 |
HeBIS-PPN: | 510563694 |
Institutes: | Biochemie, Chemie und Pharmazie |
Dewey Decimal Classification: | 5 Naturwissenschaften und Mathematik / 54 Chemie / 540 Chemie und zugeordnete Wissenschaften |
5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie | |
Sammlungen: | Universitätspublikationen |
Licence (German): | Creative Commons - CC BY-NC-ND - Namensnennung - Nicht kommerziell - Keine Bearbeitungen 4.0 International |