Halbseiten-Reaktivität der Glycerinaldehyd-3-phosphat Dehydrogenase aus Kaninchen-Skelettmuskel mit Strukturanalogen von NAD
Half-of-the-sites reactivity of glycerraldehyde-3 phosphate dehydrogenase from rabbit muscle with structural analogs of NAD
- Alkylating NAD-Analogs, Glyceraldehyde-3 Phosphate Dehydrogenase, Half-of-the-Sites Reactivity co-(3-Bromoacetylpyridinio)alkyldiphosphoadenosines with alkyl chain lengths of 2 -6 me thylene groups inactivate glyceraldehyde-3 phosphate dehydrogenase from rabbit muscle. Half-of-the-Sites reactivity is observed in each case: The analogs are covalently bound to highly reactive cysteine residues in two of the four subunits. The remaining two subunits still bind N AD and the reactive SH-groups, although modified by SH-reagents of low molecular weight are not labeled by any of the brominated coenzyme models. This behaviour may be explained by the assumption, that the modification of 2 subunits induces structural changes in the neighboured unoccupied subunits which prevent any attack on reactive cysteine residues caused by fixation and orientation of the bromoketo-coenzyme analog when bound to the active center. Structural similarities of the covalently bound coenzyme analogs in the active center and the native ternary GAPDH-NAD-substrate complex suggest that half-of-the-sites reactivity is a natural characteristic of the enzymes catalytic mechanism.
Author: | Michael EhrenfeldGND, Reinhard JeckGND, Walter Klatte, Norbert KühnGND, Christoph Woenckhaus |
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URN: | urn:nbn:de:hebis:30:3-713872 |
DOI: | https://doi.org/10.1515/znc-1981-7-806 |
ISSN: | 0939-5075 |
ISSN: | 1865-7125 |
Parent Title (German): | Zeitschrift für Naturforschung, C |
Publisher: | Verlag der Zeitschrift für Naturforschung |
Place of publication: | Tübingen |
Document Type: | Article |
Language: | German |
Date of Publication (online): | 2014/06/02 |
Year of first Publication: | 1981 |
Publishing Institution: | Universitätsbibliothek Johann Christian Senckenberg |
Release Date: | 2024/02/05 |
Tag: | Alkylating NAD-Analogs; Glyceraldehyde-3 Phosphate Dehydrogenase; Half-of-the-Sites Reactivity |
Volume: | 36.1981 |
Issue: | 7-8 |
Page Number: | 7 |
First Page: | 545 |
Last Page: | 551 |
Institutes: | Medizin |
Biochemie, Chemie und Pharmazie / Biochemie und Chemie | |
Dewey Decimal Classification: | 5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie |
Sammlungen: | Universitätspublikationen |
Licence (German): | Creative Commons - Namensnennung-Nicht kommerziell-Keine Bearbeitung 3.0 |