Characterization of structure and dynamics of the guanidine-II riboswitch from Escherichia coli by NMR spectroscopy and small-angle X-ray scattering (SAXS)
- Riboswitches are regulatory RNA elements that undergo functionally important allosteric conformational switching upon binding of specific ligands. The here investigated guanidine-II riboswitch binds the small cation, guanidinium, and forms a kissing loop-loop interaction between its P1 and P2 hairpins. We investigated the structural changes to support previous studies regarding the binding mechanism. Using NMR spectroscopy, we confirmed the structure as observed in crystal structures and we characterized the kissing loop interaction upon addition of Mg2+ and ligand for the riboswitch aptamer from Escherichia coli. We further investigated closely related mutant constructs providing further insight into functional differences between the two (different) hairpins P1 and P2. Formation of intermolecular interactions were probed by small-angle X-ray scattering (SAXS) and NMR DOSY data. All data are consistent and show the formation of oligomeric states of the riboswitch induced by Mg2+ and ligand binding.
Download full text files
Additional Services
| Author: | Tatjana SchamberORCiD, Oliver BinasORCiDGND, Andreas SchlundtORCiDGND, Anna WackerORCiDGND, Harald SchwalbeORCiDGND |
|---|---|
| URN: | urn:nbn:de:hebis:30:3-752274 |
| DOI: | https://doi.org/10.1002/cbic.202100564 |
| ISSN: | 1439-7633 |
| Parent Title (English): | ChemBioChem |
| Publisher: | Wiley-VCH |
| Place of publication: | Weinheim |
| Document Type: | Article |
| Language: | English |
| Date of Publication (online): | 2021/11/30 |
| Date of first Publication: | 2021/11/30 |
| Publishing Institution: | Universitätsbibliothek Johann Christian Senckenberg |
| Release Date: | 2023/08/28 |
| Tag: | NMR spectroscopy; RNA; aptamers; guanidine riboswitch; structural biology |
| Volume: | 23 |
| Issue: | 3, art. e202100564 |
| Article Number: | e202100564 |
| Page Number: | 9 |
| First Page: | 1 |
| Last Page: | 9 |
| Note: | Supported by the DFG in the Collaborative Research Center 902; Work at BMRZ is supported by the state of Hesse. A.S. is supported through DFG grant number SCHL2062/2-1 and by the Johanna Quandt Young Academy at Goethe (stipend number 2019/AS01). Open Access funding enabled and organized by Projekt DEAL. |
| HeBIS-PPN: | 512156050 |
| Institutes: | Biochemie, Chemie und Pharmazie |
| Biowissenschaften | |
| Wissenschaftliche Zentren und koordinierte Programme / Zentrum für Biomolekulare Magnetische Resonanz (BMRZ) | |
| Sammlungen: | Universitätspublikationen |
| Licence (German): |  Creative Commons - CC BY - Namensnennung 4.0 International |
