Cryo-EM structure of Neurospora crassa respiratory complex IV
- In fungi, the mitochondrial respiratory chain complexes (complexes I–IV) are responsible for oxidative phosphorylation, as in higher eukaryotes. Cryo-EM was used to identify a 200 kDa membrane protein from Neurospora crassa in lipid nanodiscs as cytochrome c oxidase (complex IV) and its structure was determined at 5.5 Å resolution. The map closely resembles the cryo-EM structure of complex IV from Saccharomyces cerevisiae. Its ten subunits are conserved in S. cerevisiae and Bos taurus, but other transmembrane subunits are missing. The different structure of the Cox5a subunit is typical for fungal complex IV and may affect the interaction with complex III in a respiratory supercomplex. Additional density was found between the matrix domains of the Cox4 and Cox5a subunits that appears to be specific to N. crassa.
| Author: | Thomas BauseweinGND, Stephan NußbergerORCiDGND, Werner KühlbrandtORCiDGND |
|---|---|
| URN: | urn:nbn:de:hebis:30:3-758018 |
| DOI: | https://doi.org/10.1107/S2052252519007486 |
| ISSN: | 2052-2525 |
| Parent Title (English): | IUCrJ |
| Publisher: | International Union of Crystallography |
| Place of publication: | Chester |
| Document Type: | Article |
| Language: | English |
| Date of Publication (online): | 2019/09/26 |
| Date of first Publication: | 2019/09/26 |
| Publishing Institution: | Universitätsbibliothek Johann Christian Senckenberg |
| Release Date: | 2024/05/02 |
| Tag: | 3D reconstruction and image processing; Neurospora crassa; automation; cryo-electron microscopy; cytochrome c oxidase; respiratory complex IV; single-particle cryo-EM; structure determination |
| Volume: | 6 |
| Issue: | 4 |
| Page Number: | 8 |
| First Page: | 773 |
| Last Page: | 780 |
| Institutes: | Biochemie, Chemie und Pharmazie / Biochemie und Chemie |
| Angeschlossene und kooperierende Institutionen / MPI für Biophysik | |
| Dewey Decimal Classification: | 5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie |
| Sammlungen: | Universitätspublikationen |
| Licence (German): |  Creative Commons - Namensnennung 4.0 |
