Georg Tascher, Audrey Burban, Sandrine Camus, Marine Plumel, Stéphanie Chanon, Rémy Le Guével, Valery Shevchenko, Alain Van Dorsselaer, Etienne Lefai, Christiane Guguen-Guillouzo, Fabrice Bertile
- Of the hepatic cell lines developed for in vitro studies of hepatic functions as alternatives to primary human hepatocytes, many have lost major liver-like functions, but not HepaRG cells. The increasing use of the latter worldwide raises the need for establishing the reference functional status of early biobanked HepaRG cells. Using deep proteome and secretome analyses, the levels of master regulators of the hepatic phenotype and of the structural elements ensuring biliary polarity were found to be close to those in primary hepatocytes. HepaRG cells proved to be highly differentiated, with functional mitochondria, hepatokine secretion abilities, and an adequate response to insulin. Among differences between primary human hepatocytes and HepaRG cells, the factors that possibly support HepaRG transdifferentiation properties are discussed. The HepaRG cell system thus appears as a robust surrogate for primary hepatocytes, which is versatile enough to study not only xenobiotic detoxification, but also the control of hepatic energy metabolism, secretory function and disease-related mechanisms.
MetadatenAuthor: | Georg TascherORCiDGND, Audrey Burban, Sandrine Camus, Marine Plumel, Stéphanie Chanon, Rémy Le Guével, Valery Shevchenko, Alain Van Dorsselaer, Etienne Lefai, Christiane Guguen-Guillouzo, Fabrice Bertile |
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URN: | urn:nbn:de:hebis:30:3-514682 |
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DOI: | https://doi.org/10.3390/cells8020192 |
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ISSN: | 2073-4409 |
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Pubmed Id: | https://pubmed.ncbi.nlm.nih.gov/30795634 |
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Parent Title (English): | Cells |
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Publisher: | MDPI |
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Place of publication: | Basel |
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Document Type: | Article |
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Language: | English |
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Year of Completion: | 2019 |
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Date of first Publication: | 2019/02/21 |
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Publishing Institution: | Universitätsbibliothek Johann Christian Senckenberg |
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Release Date: | 2019/10/28 |
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Tag: | HepaRG cells; Hepatocytes; detoxification; hepatic phenotype; liver cell lines; liver diseases; liver metabolism; proteome; secretome; transdifferentiation |
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Volume: | 8 |
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Issue: | 2, Art. 192 |
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Page Number: | 25 |
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First Page: | 1 |
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Last Page: | 25 |
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Note: | This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited |
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HeBIS-PPN: | 455331669 |
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Institutes: | Medizin / Medizin |
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Dewey Decimal Classification: | 5 Naturwissenschaften und Mathematik / 54 Chemie / 540 Chemie und zugeordnete Wissenschaften |
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| 5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie |
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Sammlungen: | Universitätspublikationen |
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Licence (German): | Creative Commons - Namensnennung 4.0 |
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