Parkinson phenotype in aged PINK1-deficient mice is accompanied by progressive mitochondrial dysfunction in absence of neurodegeneration

  • Background Parkinson's disease (PD) is an adult-onset movement disorder of largely unknown etiology. We have previously shown that loss-of-function mutations of the mitochondrial protein kinase PINK1 (PTEN induced putative kinase 1) cause the recessive PARK6 variant of PD. Methodology/Principal Findings Now we generated a PINK1 deficient mouse and observed several novel phenotypes: A progressive reduction of weight and of locomotor activity selectively for spontaneous movements occurred at old age. As in PD, abnormal dopamine levels in the aged nigrostriatal projection accompanied the reduced movements. Possibly in line with the PARK6 syndrome but in contrast to sporadic PD, a reduced lifespan, dysfunction of brainstem and sympathetic nerves, visible aggregates of alpha-synuclein within Lewy bodies or nigrostriatal neurodegeneration were not present in aged PINK1-deficient mice. However, we demonstrate PINK1 mutant mice to exhibit a progressive reduction in mitochondrial preprotein import correlating with defects of core mitochondrial functions like ATP-generation and respiration. In contrast to the strong effect of PINK1 on mitochondrial dynamics in Drosophila melanogaster and in spite of reduced expression of fission factor Mtp18, we show reduced fission and increased aggregation of mitochondria only under stress in PINK1-deficient mouse neurons. Conclusion Thus, aging Pink1 -/- mice show increasing mitochondrial dysfunction resulting in impaired neural activity similar to PD, in absence of overt neuronal death.

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Author:Suzana Gispert, Filomena Ricciardi, Alexander Kurz, Mekhman Azizov, Hans-Hermann Hoepken, Dorothea Becker, Wolfgang Voos, Kristina Leuner, Walter E. MüllerGND, Alexei P. Kudin, Wolfram S. Kunz, Annabelle Zimmermann, Jochen RoeperORCiD, Dirk Wenzel, Marina Jendrach, Moisés García-Arencíbia, Javier Fernández-Ruiz, Leslie Huber, Hermann RohrerORCiD, Miguel Barrera, Andreas ReichertORCiDGND, Udo Rüb, Amy Chen, Robert L. Nussbaum, Georg AuburgerORCiDGND
Parent Title (English):PLoS One
Document Type:Article
Date of Publication (online):2009/06/03
Date of first Publication:2009/06/03
Publishing Institution:Universitätsbibliothek Johann Christian Senckenberg
Release Date:2009/08/11
Issue:(6): e5777
This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.
Source:PLoS ONE 4(6): e5777. ; doi:10.1371/journal.pone.0005777
Institutes:Medizin / Medizin
Dewey Decimal Classification:6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit
Licence (German):License LogoDeutsches Urheberrecht