ADAM10 is expressed in human podocytes and found in urinary vesicles of patients with glomerular kidney diseases

  • Background: The importance of the Notch signaling in the development of glomerular diseases has been recently described. Therefore we analyzed in podocytes the expression and activity of ADAM10, one important component of the Notch signaling complex. Methods: By Western blot, immunofluorescence and immunohistochemistry analysis we characterized the expression of ADAM10 in human podocytes, human urine and human renal tissue. Results: We present evidence, that differentiated human podocytes possessed increased amounts of mature ADAM10 and released elevated levels of L1 adhesion molecule, one well known substrate of ADAM10. By using specific siRNA and metalloproteinase inhibitors we demonstrate that ADAM10 is involved in the cleavage of L1 in human podocytes. Injury of podocytes enhanced the ADAM10 mediated cleavage of L1. In addition, we detected ADAM10 in urinary podocytes from patients with kidney diseases and in tissue sections of normal human kidney. Finally, we found elevated levels of ADAM10 in urinary vesicles of patients with glomerular kidney diseases. Conclusions: The activity of ADAM10 in human podocytes may play an important role in the development of glomerular kidney diseases.

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Author:Paul GutweinGND, Anja Schramme, Mohamed Sadek Abdel-Bakky, Kai Doberstein, Ingeborg A. HauserGND, Andreas Ludwig, Peter Altevogt, Stefan GauerGND, Anja Hillmann, Thomas Weide, Christine Jespersen, Wolfgang EberhardtGND, Josef PfeilschifterGND
Pubmed Id:
Parent Title (English):Journal of biomedical science
Publisher:BioMed Central
Place of publication:London
Document Type:Article
Date of Publication (online):2010/03/10
Date of first Publication:2010/01/13
Publishing Institution:Universitätsbibliothek Johann Christian Senckenberg
Release Date:2010/03/10
Issue:1, Art. 3
Page Number:9
First Page:1
Last Page:9
© 2010 Gutwein et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Source:Journal of Biomedical Science 2010, 17:3 ; doi:10.1186/1423-0127-17-3 ;
Institutes:Medizin / Medizin
Dewey Decimal Classification:6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit
Licence (German):License LogoCreative Commons - Namensnennung 2.0