Yeast Rrp8p, a novel methyltransferase responsible for m1A 645 base modification of 25S rRNA
- Ribosomal RNA undergoes various modifications to optimize ribosomal structure and expand the topological potential of RNA. The most common nucleotide modifications in ribosomal RNA (rRNA) are pseudouridylations and 2'-O methylations (Nm), performed by H/ACA box snoRNAs and C/D box snoRNAs, respectively. Furthermore, rRNAs of both ribosomal subunits also contain various base modifications, which are catalysed by specific enzymes. These modifications cluster in highly conserved areas of the ribosome. Although most enzymes catalysing 18S rRNA base modifications have been identified, little is known about the 25S rRNA base modifications. The m(1)A modification at position 645 in Helix 25.1 is highly conserved in eukaryotes. Helix formation in this region of the 25S rRNA might be a prerequisite for a correct topological framework for 5.8S rRNA to interact with 25S rRNA. Surprisingly, we have identified ribosomal RNA processing protein 8 (Rrp8), a nucleolar Rossman-fold like methyltransferase, to carry out the m(1)A base modification at position 645, although Rrp8 was previously shown to be involved in A2 cleavage and 40S biogenesis. In addition, we were able to identify specific point mutations in Rrp8, which show that a reduced S-adenosyl-methionine binding influences the quality of the 60S subunit. This highlights the dual functionality of Rrp8 in the biogenesis of both subunits.
Author: | Christian PeiferORCiDGND, Sunny SharmaORCiDGND, Peter Watzinger, Stefanie Lamberth, Peter KötterORCiD, Karl-Dieter Entian |
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URN: | urn:nbn:de:hebis:30:3-270783 |
URL: | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3553958/ |
DOI: | https://doi.org/10.1093/nar/gks1102 |
ISSN: | 0301-5610 |
ISSN: | 0305-1048 |
ISSN: | 1362-4962 |
Pubmed Id: | https://pubmed.ncbi.nlm.nih.gov/23180764 |
Parent Title (English): | Nucleic acids research |
Publisher: | Oxford Univ. Press |
Place of publication: | Oxford |
Document Type: | Article |
Language: | English |
Date of Publication (online): | 2012/11/29 |
Date of first Publication: | 2012/11/23 |
Publishing Institution: | Universitätsbibliothek Johann Christian Senckenberg |
Release Date: | 2012/11/29 |
Volume: | 40 |
Issue: | 2 |
Page Number: | 13 |
First Page: | 1151 |
Last Page: | 1163 |
Note: | © The Author(s) 2012. Published by Oxford University Press. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/3.0/), which permits non-commercial reuse, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com. |
HeBIS-PPN: | 314221727 |
Institutes: | Biowissenschaften / Biowissenschaften |
Exzellenzcluster / Exzellenzcluster Makromolekulare Komplexe | |
Dewey Decimal Classification: | 5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie |
Sammlungen: | Universitätspublikationen |
Sammlung Biologie / Sondersammelgebiets-Volltexte | |
Licence (German): | Creative Commons - Namensnennung-Nicht kommerziell 3.0 |