Resonance Raman and FTIR spectroscopic characterization of the closed and open states of channelrhodopsin-1
- Channelrhodopsin-1 from Chlamydomonas augustae (CaChR1) is a light-activated cation channel, which is a promising optogenetic tool. We show by resonance Raman spectroscopy and retinal extraction followed by high pressure liquid chromatography (HPLC) that the isomeric ratio of all-trans to 13-cis of solubilized channelrhodopsin-1 is with 70:30 identical to channelrhodopsin-2 from Chlamydomonas reinhardtii (CrChR2). Critical frequency shifts in the retinal vibrations are identified in the Raman spectrum upon transition to the open (conductive P2(380)) state. Fourier transform infrared spectroscopy (FTIR) spectra indicate different structures of the open states in the two channelrhodopsins as reflected by the amide I bands and the protonation pattern of acidic amino acids.
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| Author: | Vera Muders, Silke Kerruth, Víctor A. Lórenz-Fonfría, Christian BamannORCiDGND, Joachim Heberle, Ramona Schlesinger |
|---|---|
| URN: | urn:nbn:de:hebis:30:3-349784 |
| DOI: | https://doi.org/10.1016/j.febslet.2014.05.019 |
| ISSN: | 1873-3468 |
| ISSN: | 0014-5793 |
| Pubmed Id: | https://pubmed.ncbi.nlm.nih.gov/24859039 |
| Parent Title (English): | FEBS Letters |
| Publisher: | Elsevier |
| Place of publication: | Amsterdam [u.a.] |
| Document Type: | Article |
| Language: | English |
| Date of Publication (online): | 2014/05/21 |
| Date of first Publication: | 2014/05/21 |
| Publishing Institution: | Universitätsbibliothek Johann Christian Senckenberg |
| Release Date: | 2014/09/17 |
| Tag: | CaChR1; Channelrhodopsin; Optogenetics; Photoreceptor; Resonance Raman spectroscopy; Retinal |
| Volume: | 588 |
| Issue: | 14 |
| Page Number: | 6 |
| First Page: | 2301 |
| Last Page: | 2306 |
| Note: | (c) 2014 The Authors. Published by Elsevier B.V. on behalf of the Federation of European Biochemical Societies. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/3.0/). |
| HeBIS-PPN: | 366224328 |
| Institutes: | Biochemie, Chemie und Pharmazie / Biochemie und Chemie |
| Dewey Decimal Classification: | 5 Naturwissenschaften und Mathematik / 54 Chemie / 540 Chemie und zugeordnete Wissenschaften |
| 5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie | |
| Sammlungen: | Universitätspublikationen |
| Licence (German): |  Creative Commons - Namensnennung-Nicht kommerziell-Keine Bearbeitung 3.0 |
